HDAC1 Polyclonal Antibody for Western Blot, IF, ICC, IHC, IHC (F), IP and ChIP
HDAC1 Antibody (PA1-860) in IF
Immunofluorescent analysis of HDAC1 (green) in 3T3 cells. The cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 15 minutes at room temperature. Cells were stained with a HDAC1 rabbit polyclonal antibody (Product # PA1-860), at a concentration of 10ug/ml in blocking buffer for at least 1 hour at room temperature, and then incubated with a Goat anti-rabbit IgG Superclonal secondary antibody, Alexa Fluor 488 conjugate (Product # A27034) at a dilution of 1:1000 for 30 minutes at room temperature (green). Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.