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Phospho-FAK2 (Tyr402) Polyclonal Antibody

发表于2017年5月3日由上海金畔生物科技有限公司

Phospho-FAK2 (Tyr402) Polyclonal Antibody for Western Blot, IF, ICC, IHC (P) and Flow

Phospho-FAK2 (Tyr402) Polyclonal Antibody Phospho-FAK2 (Tyr402) Polyclonal Antibody Phospho-FAK2 (Tyr402) Polyclonal Antibody Phospho-FAK2 (Tyr402) Polyclonal Antibody Phospho-FAK2 (Tyr402) Polyclonal Antibody Phospho-FAK2 (Tyr402) Polyclonal Antibody Phospho-FAK2 (Tyr402) Polyclonal Antibody Phospho-FAK2 (Tyr402) Polyclonal Antibody

Phospho-FAK2 (Tyr402) Polyclonal Antibody

Phospho-FAK2 (Tyr402) Antibody (44-618G) in IF

Immunofluorescence analysis of PYK2 [pY402] was done on 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with PYK2 [pY402] Rabbit Polyclonal Antibody (44618G) at 1ug/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

Performance guaranteed

Purchase with confidence, knowing that we stand behind the performance of our antibodies with the InvitrogenTM antibody performance guarantee. If an InvitrogenTM antibody doesn’t perform in your experiment as described on our website or datasheet, we’ll replace the product at no cost to you, or if you prefer, we’ll provide you with a credit for a future purchase.* Catalog#: 44-618G Invitrogen Phospho-FAK2 (Tyr402) Polyclonal Antibody

Details
Tested species reactivity Chicken, Human, Mouse, Rat
Published species reactivity Tag, Rat, Human, Mouse, Not Applicable
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Pyk2 that contains tyrosine 402. The sequence is conserved in rat.
Conjugate Unconjugated
Form Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco’s PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA
Contains 0.05% sodium azide
Storage Conditions -20°C

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原文地址:http://www.thermofisher.com/antibody/product/Phospho-FAK2-Tyr402-Antibody-Polyclonal/44-618G

此条目是由上海金畔生物科技有限公司发表在未分类分类目录的。将固定链接加入收藏夹。
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