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Calreticulin Polyclonal Antibody

发表于2017年5月3日由上海金畔生物科技有限公司

Calreticulin Polyclonal Antibody for Western Blot, IF, ICC, Flow and IP

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Calreticulin Polyclonal Antibody

Calreticulin Antibody (PA3-900) in IF

Immunofluorescent analysis of Calreticulin (green) U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS 0.1% triton-X (Product #37525) for 30 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing Calreticulin (Product # PA3-900) at a dilution of 1:50 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody (Product #35552) at a dilution of 1:250 for 30 minutes at room temperature. Actin filaments (red) were stained with DyLight 554-Phalloidin (Product# 21834) at a dilution of 1:300 (i.e. 1unit/mL final concentration) in PBS and incubated for 30 min including. Nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249) at a dilution of 1µg/mL. Images were taken on a Thermo Scientific ArrayScan VTI at 20X magnification.

Performance guaranteed

Purchase with confidence, knowing that we stand behind the performance of our antibodies with the InvitrogenTM antibody performance guarantee. If an InvitrogenTM antibody doesn’t perform in your experiment as described on our website or datasheet, we’ll replace the product at no cost to you, or if you prefer, we’ll provide you with a credit for a future purchase.* Catalog#: PA3-900 Invitrogen Calreticulin Polyclonal Antibody

Details
Tested species reactivity Dog, Human, Mouse, Non-human primate, Rabbit, Rat
Published species reactivity Dog, Rat, Non-human primate, Virus, Amphibian, Bovine, Hamster, Mouse, Human, Echinoderm, Not Applicable
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant, human calreticulin protein.
Conjugate Unconjugated
Form Liquid
Storage buffer whole serum
Contains 0.05% sodium azide
Storage Conditions -20° C, Avoid Freeze/Thaw Cycles

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原文地址:http://www.thermofisher.com/antibody/product/Calreticulin-Antibody-Polyclonal/PA3-900

此条目是由上海金畔生物科技有限公司发表在未分类分类目录的。将固定链接加入收藏夹。
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