Cy5

 
                                 结构                                                                  未结合的Cy5染料在磷酸盐缓冲盐水中的激发和发射光谱

产品属性

Excitation maximum (nm)：646

Emission maximum (nm) ：664

Extinction coefficient (M-1 cm-1)：250000

Quantum yield：0.27

Fluorescence lifetime (ns)：1.0

附着于生物分子（例如寡核苷酸）后，观察到激发和发射波长的变化可忽略不计。 此外，在10％DMSO存在下或在一定pH条件下，未观察到对这些光谱特征的影响。

  
                                Structure                                              Excitation and emission spectra of unconjugated Cy5 dye in phosphate buffered saline.

Properties

Excitation maximum (nm)：646

Emission maximum (nm) ：664

Extinction coefficient (M-1 cm-1)：250000

Quantum yield：0.27

Fluorescence lifetime (ns)：1.0

Upon attachment to biomolecules (for example oligonucleotides), negligible shifts in excitation and emission wavelength have been observed. In addition no effects on these spectral characteristics have been observed in the presence of 10% DMSO or under a range of pH conditions.