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Phospho-SMAD2 (Ser465, Ser467) Polyclonal Antibody

发表于2017年5月6日由上海金畔生物科技有限公司

Phospho-SMAD2 (Ser465, Ser467) Polyclonal Antibody for Western Blot, IF, ICC, IHC (P), Flow and ChIP

Phospho-SMAD2 (Ser465, Ser467) Polyclonal Antibody Phospho-SMAD2 (Ser465, Ser467) Polyclonal Antibody Phospho-SMAD2 (Ser465, Ser467) Polyclonal Antibody Phospho-SMAD2 (Ser465, Ser467) Polyclonal Antibody Phospho-SMAD2 (Ser465, Ser467) Polyclonal Antibody Phospho-SMAD2 (Ser465, Ser467) Polyclonal Antibody Phospho-SMAD2 (Ser465, Ser467) Polyclonal Antibody

Phospho-SMAD2 (Ser465, Ser467) Polyclonal Antibody

Phospho-SMAD2 (Ser465, Ser467) Antibody (44-244G) in IF

Immunofluorescence analysis of Phospho-SMAD2 pSer465 / pSer467 Antibody was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were treated with TGF-beta1(20ng/mL) for 15 minutes and labelled with Phospho-SMAD2 pSer465 / pSer467 Antibody(44244G) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa flour 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing Nuclear localization. Panel e showing merged image of untreated cells with less nuclear signals Panel f is a no primary antibody control. The images were captured at 40X magnification.

Performance guaranteed

Purchase with confidence, knowing that we stand behind the performance of our antibodies with the InvitrogenTM antibody performance guarantee. If an InvitrogenTM antibody doesn’t perform in your experiment as described on our website or datasheet, we’ll replace the product at no cost to you, or if you prefer, we’ll provide you with a credit for a future purchase.* Catalog#: 44-244G Invitrogen Phospho-SMAD2 (Ser465, Ser467) Polyclonal Antibody

Details
Tested species reactivity Human, Mouse
Published species reactivity Human, Mouse, Not Applicable
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human Smad2 that contains serines 465 and 467. The sequence is conserved in mouse and rat.
Conjugate Unconjugated
Form Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco’s PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol
Contains 0.05% sodium azide
Storage Conditions -20°C

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原文地址:http://www.thermofisher.com/antibody/product/Phospho-SMAD2-Ser465-Ser467-Antibody-Polyclonal/44-244G

此条目是由上海金畔生物科技有限公司发表在未分类分类目录的。将固定链接加入收藏夹。
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