链霉亲合素
基本信息
| 产品名称 | 链霉亲合素 |
|---|---|
| 英文名称 | Streptavidin and Conjugates |
| 别名 | 链亲合素 |
| 英文别名 | Streptavidin from Streptomyces avidinii;Streptavidin and Conjugates |
| 应用 | Applications for enzyme-labeled (HRP, AP) streptavidin: Enzyme immunoassays (EIA), including enzyme-linked immunsorbent assays (ELISA) with biotinylated antibodies Cell and tissue staining (for light microscopy) Western blotting (WB) and other membrane-based biotin detection Applications for fluorescent (FITC, TRITC or Texas Red* Fluor-labeled) streptavidin: Immunofluorescent assays (IFA) Tissue staining (for fluorescence microscopy) Blot immunostaining Cell staining (incl. fluorescent activated cell sorting) |
| 规格或纯度 | from Streptomyces avidinii,≥12 U/mg protein |
| 运输条件 | 超低温冰袋运输 |
| 生化机理 | reptavidin is biotin-binding protein that was originally isolated from Streptomyces avidinii. In contrast to avidin, streptavidin has no carbohydrate and has a mildly acidic pI of 5. OursStreptavidin products use a recombinant form of streptavidin having a mass of 53,000 daltons and a near-neutral pI. Streptavidin is a tetrameric protein, with each subunit binding one molecule of biotin with affinity similar to that of avidin. Guanidinium chloride will dissociate avidin and streptavidin into subunits, but streptavidin is more resistant to dissociation. |
一般描述
Streptavidin and Conjμgates include streptavidin in a purified form, conjμgated to enzymes or fluorophores or activated for crosslinking to sulfhydryl or carbonyl groups. These purified and conjμgated forms of recombinant streptavidin protein enable detection of biotinylated antibodies and other probes in a variety of standard assay methods, including Western blotting, ELISA, immunohistochemistry and fluorescence imaging. Purified streptavidin and the streptavidin-HRP conjμgates (peroxidase-conjμgated streptavidin) are supplied lyophilized in phosphate-based buffers for immediate reconstitution with water. Alkaline Phosphatase conjμgates are supplied as a liquid in a Tris-based buffer. Applications for enzyme-labeled (HRP, AP) streptavidin: Enzyme immunoassays (EIA), including enzyme-linked immunsorbent assays (ELISA) with biotinylated antibodies Cell and tissue staining (for light microscopy) Western blotting (WB) and other membrane-based biotin detection Applications for fluorescent (FITC, TRITC or Texas Red* Fluor-labeled) streptavidin: Immunofluorescent assays (IFA) Tissue staining (for fluorescence microscopy) Blot immunostaining Cell staining (incl. fluorescent activated cell sorting)
Streptavidin and Conjugates include streptavidin in a purified form, conjugated to enzymes or fluorophores or activated for crosslinking to sulfhydryl or carbonyl groups. These purified and conjugated forms of recombinant streptavidin protein enable detection of biotinylated antibodies and other probes in a variety of standard assay methods, including Western blotting, ELISA, immunohistochemistry and fluorescence imaging. Purified streptavidin and the streptavidin-HRP conjugates (peroxidase-conjugated streptavidin) are supplied lyophilized in phosphate-based buffers for immediate reconstitution with water. Alkaline Phosphatase conjugates are supplied as a liquid in a Tris-based buffer.
Applications for enzyme-labeled (HRP, AP) streptavidin: Enzyme immunoassays (EIA), including enzyme-linked immunsorbent assays (ELISA) with biotinylated antibodies Cell and tissue staining (for light microscopy) Western blotting (WB) and other membrane-based biotin detection Applications for fluorescent (FITC, TRITC or Texas Red* Fluor-labeled) streptavidin: Immunofluorescent assays (IFA) Tissue staining (for fluorescence microscopy) Blot immunostaining Cell staining (incl. fluorescent activated cell sorting)
相关属性
| CAS编号 | 9013-20-1 |
|---|---|
| 敏感性 | 对热敏感、易吸潮 |
| 储存温度 | -20°C储存,充氩 |
| MDL号 | MFCD00082035 |
| 品牌 | Jinpan |
| PubChem CID | 51062757 |