beta Actin Loading Control Monoclonal Antibody for Western Blot, IHC (P), Flow and ELISA
beta Actin Loading Control Antibody (MA5-15739) in IHC (P)
Immunohistochemistry analysis of Beta Actin showing staining in the cytoskeleton of paraffin-embedded human skeletal muscle tissue (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Beta Actin loading control antibody (Product # MA5-15739) diluted in 3% BSA-PBS at a dilution of 1:200 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.