PSD95 Monoclonal Antibody for Western Blot, IF, ICC, Flow and IP
PSD95 Antibody (MA1-045) in IF
Immunofluorescent analysis of Post Synaptic Density 95kD protein (PSD95, green) in cultured primary cortical neurons. Primary cortical neurons are isolated and cultured using the Primary Neuron Isolation Kit (Product # 88280). At day 28, neurons were fixed with 4% paraformaldehyde, permeablilized with 0.1% triton X-100 in HBSS for 10 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were probed with a PSD95 monoclonal antibody (Product # MA1-045) at a dilution of 1:500 for 2 hours at room temperature or overnight at 4 °C, washed with HBSS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product# 35502) at dilution of 1:500 for 1 hour at room temperature. Images were taken at 20X magnification on a Carl Zeiss microscopy (AxioVision Rel. 4.7).