Nestin Monoclonal Antibody for Western Blot, IF, ICC, IHC (P) and Flow
Nestin Antibody (MA1-110) in IF
Immunofluorescent analysis of Nestin (green) in human neural stem cells derived from PD-3 iPSCs using Gibco® PSC Neural Induction Medium (Product # A1647801). The cells were fixed and permeabilized using Image-IT® Fixation/Permeabilization kit (Product # R37602), and blocked with the including blocking buffer for one hour at room temperature. Cells were stained with a Nestin monoclonal antibody (Product # MA1-110) at a dilution of 1:100 in blocking buffer for 3 hours at room temperature, and then incubated with a DyLight488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 1 hour at room temperature. Nucleus DNA (blue) was stained with DAPI (Product # D1306). Images were taken on an EVOS® FLoid® Cell Imaging Station at 10X magnification.