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Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 594

发表于2017年5月2日由上海金畔生物科技有限公司

Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 594 conjugate for IF, ICC and Flow

Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 594 Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 594 Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 594 Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 594 Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 594

Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 594

Rabbit IgG (H+L) Secondary Antibody (R37117) in IF

CAKI cells were plated on coverslips overnight. The next day cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602) according to protocol. Following suppression of background binding using Image-iT® FX Signal enhancer (R37107), cells were incubated with 3 µg/mL anti-ATP synthase subunit IF1 (A21355) for labeling of mitochondria for 30 min at room temperature and washed three times with dPBS, followed by Alexa Fluor® 594 goat anti-rabbit IgG antibody ReadyProbes® reagent (R37117) for 30 min and washed three times in dPBS. Cells were labeled with NucBlue® Live (R37605) and ActinGreen™ 488 ReadyProbes® reagent (R37110) according to protocol. Coverslips were then mounted using ProLong® Gold antifade reagent (P36930). Individual images were acquired on the EVOS® FL Auto imaging system with a 10X objective (AMEP4681) and stitched to a panorama using the tile/stitch function.

Performance guaranteed

Purchase with confidence, knowing that we stand behind the performance of our antibodies with the InvitrogenTM antibody performance guarantee. If an InvitrogenTM antibody doesn’t perform in your experiment as described on our website or datasheet, we’ll replace the product at no cost to you, or if you prefer, we’ll provide you with a credit for a future purchase.* Catalog#: R37117 Invitrogen Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 594

Details
Tested species reactivity Rabbit
Host / Isotype Goat / IgG
Class Polyclonal
Type Secondary Antibody
Immunogen Gamma Immunoglobin
Conjugate Alexa Fluor® 594
Form Liquid
Purification Affinity chromatography
Storage buffer PBS with 0.1% BSA
Contains <0.1% sodium azide
Storage Conditions 4° C, store in dark
Target IgG
Cross Adsorption Against bovine IgG, goat IgG, mouse IgG, rat IgG and human IgG
Antibody Form Whole Antibody

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原文地址:http://www.thermofisher.com/antibody/product/Goat-anti-Rabbit-IgG-H-L-Secondary-Antibody-Polyclonal/R37117

此条目是由上海金畔生物科技有限公司发表在未分类分类目录的。将固定链接加入收藏夹。
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