Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 594 conjugate for IF, ICC and Flow
Rabbit IgG (H+L) Secondary Antibody (R37117) in IF
CAKI cells were plated on coverslips overnight. The next day cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602) according to protocol. Following suppression of background binding using Image-iT® FX Signal enhancer (R37107), cells were incubated with 3 µg/mL anti-ATP synthase subunit IF1 (A21355) for labeling of mitochondria for 30 min at room temperature and washed three times with dPBS, followed by Alexa Fluor® 594 goat anti-rabbit IgG antibody ReadyProbes® reagent (R37117) for 30 min and washed three times in dPBS. Cells were labeled with NucBlue® Live (R37605) and ActinGreen™ 488 ReadyProbes® reagent (R37110) according to protocol. Coverslips were then mounted using ProLong® Gold antifade reagent (P36930). Individual images were acquired on the EVOS® FL Auto imaging system with a 10X objective (AMEP4681) and stitched to a panorama using the tile/stitch function.