Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor® 555 conjugate for IF, ICC and Flow
Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A-21424) in IF
Four-color fluorescence in situ hybridization on a Drosophila embryo. A late blastoderm stage (nuclear cycle 14) embryo was probed with four different RNA probes. Blue: sog labeled with DNP, followed by a rabbit anti-dinitrophenyl-KLH IgG antibody (Prod # A6430) detected with an Alexa Fluor® 647 chicken anti-rabbit IgG antibody (Prod # A21443). Green: ind labeled with biotin, followed by streptavidin HRP and Alexa Fluor® 350 tyramide (TSA Kit #27, Prod # T20937). Red: msh labeled with digoxigenin followed by sheep anti-digoxigenin antibody detected with an Alexa Fluor® 488 donkey anti-sheep IgG antibody (Prod # A11015). Yellow: sna labeled with fluorescein followed by mouse anti-fluorescein antibody detected with an Alexa Fluor® 555 goat anti-mouse IgG antibody (Prod # A21424). Image contributed by Dave Kosman and Ethan Bier, University of California, San Diego.