Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor® 594 conjugate for IF, ICC and Flow
Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A-11032) in IF
Immunofluorescence was performed on methanol fixed HeLa cells for detection of BNIP3 using Anti-BNIP3 (8HCLC) Recombinant Rabbit Oligoclonal Antibody (Product# 710728, 2ug/ml), alpha-Tubulin Monoclonal Antibody (Product# 322500, 1ug/ml) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000), Goat anti-Mouse IgG Secondary Antibody, Alexa Fluor® 594 conjugate (Product # A11032, 1:400) respectively. Panel a) shows representative cells that were stained for detection and localization of BNIP3 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938,). Panel c) represents cytoskeletal alpha-tubulin staining (red). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of BNIP3. Panel e) represents merged image of untreated cells with no signal Panel f) represents control cells with no primary Antibody to assess background.