Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor® 488 conjugate for IF, ICC and Flow
Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A-11029) in IF
Simultaneous detection of expression of five genes in a whole-mount Drosophila embryo by fluorescence in situ hybridization (FISH) with five RNA probes. Red: sog labeled using aminoallyl UTP (Prod # A21663, A32765) and Alexa Fluor® 647 succinimidyl ester (Prod # A20006, A20106). Green: ind labeled with DNP, followed by rabbit anti-dinitrophenyl-KLH IgG antibody (Prod # A6430) prelabeled with the Zenon® Alexa Fluor® 555 Rabbit IgG Labeling Kit (Prod # Z25305). Blue: en labeled with biotin and detected with HRP-streptavidin and Alexa Fluor® 405 tyramide (TSA™ Kit #39, T30952). Yellow: wg labeled with digoxigenin and detected with sheep anti-digoxigenin IgG antibody and Alexa Fluor® 594 Donkey Anti-Sheep IgG antibody (Prod # A11016). Magenta: msh labeled with fluorescein and detected with mouse anti-fluorescein/Oregon Green® IgG<sub>2a</sub> antibody (Prod # A6421) and Alexa Fluor® 488 Goat Anti-Mouse IgG antibody (Prod # A11001, A11029). Image contributed by Dave Kosman and Ethan Bier, University of California, San Diego.