Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor® Plus 647 conjugate for Western Blot, IF and ICC
Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A32733) in IF
Immunofluorescent analysis of postsynaptic density protein (PSD-95) in E18 Sparague Dawley primary cortical neuronal cells. The cells were fixed with 4% formaldehyde for 15 mins, permeabilized with 0.25% Triton X-100 in PBS for 10 mins, and blocked with 3% BSA in PBS for 30 mins at RT. Cells were stained with a PSD-95 rabbit polyclonal antibody (Product # 51-6900) at a dilution of 1:200 in 3% BSA in PBS for 1 hr at RT, and then incubated with Invitrogen Alexa Fluor Plus 647 goat anti-rabbit IgG secondary antibody (Product # A32733) at a dilution of 1:1000 for 1 hr at RT. Nuclei were stained with Hoechst 33342 (product # H3570). The image contains overlay of neuronal PSD-95 (far red) and nuclei (blue). Images were taken on a Zeiss LSM 710 confocal microscope at 40X magnification.