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Occludin Polyclonal Antibody

发表于2017年5月3日由上海金畔生物科技有限公司

Occludin Polyclonal Antibody for Western Blot, IF, ICC, IHC, IP and ELISA

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Occludin Polyclonal Antibody

Occludin Antibody (71-1500) in IF

Immunofluorescence analysis of Occludin was performed using 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Occludin Rabbit Polyclonal Antibody (711500) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cell junctional localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Performance guaranteed

Purchase with confidence, knowing that we stand behind the performance of our antibodies with the InvitrogenTM antibody performance guarantee. If an InvitrogenTM antibody doesn’t perform in your experiment as described on our website or datasheet, we’ll replace the product at no cost to you, or if you prefer, we’ll provide you with a credit for a future purchase.* Catalog#: 71-1500 Invitrogen Occludin Polyclonal Antibody

Details
Tested species reactivity Dog, Human, Rat
Published species reactivity Dog, Baboon, Rabbit, Rat, Bacteria, Mouse, Human, Chicken, Not Applicable
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen A fusion protein containing the C-terminal 150 amino acid region of human occludin.
Conjugate Unconjugated
Form Liquid
Concentration 0.25 mg/ml
Purification Antigen affinity chromatography
Storage buffer PBS, pH 7.4
Contains 0.1% sodium azide
Storage Conditions -20°C

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原文地址:http://www.thermofisher.com/antibody/product/Occludin-Antibody-Polyclonal/71-1500

此条目是由上海金畔生物科技有限公司发表在未分类分类目录的。将固定链接加入收藏夹。
沪ICP备14053477号-16 上海金畔生物科技有限公司