异丙基-β-D-硫代半乳糖苷（IPTG）
基本信息
| 产品名称 |
异丙基-β-D-硫代半乳糖苷（IPTG） |
| 英文名称 |
Isopropyl beta-D-thiogalactoside |
| 别名 |
IPTG；异丙基1-硫代-β-D-半乳糖吡喃糖苷 |
| 英文别名 |
IPTG; Isopropyl 1-Thio-β-D-galactopyranoside; Isopropyl-β-D-thiogalactopyranoside |
| 应用 |
IPTG 常用于需要诱导 β-半乳糖苷酶活性的克隆实验。它常与 X-Gal 或 Bluo-Gal 结合使用，用于重组细菌菌落的蓝白筛选，这些菌落可以诱导 lac 操纵子在大肠杆菌中的表达。IPTG 与 lacI 阻遏蛋白结合并改变其构象而发挥作用，防止 β-半乳糖苷酶编码基因 lacZ 的抑制。 |
| 规格或纯度 |
>98.0% |
| 运输条件 |
冰袋运输 |
| 生化机理 |
与X-GAL结合使用，用于需要表达β-半乳糖苷酶活性的克隆程序中。半乳糖的不可代谢类似物。 |
一般描述
IPTG, Dioxane-Free (isopropyl-β-thiogalactoside) is an analogue of allolactose that binds specifically to the repressor protein of the lac operon and induces expression of β-galactosidase in Escherichia coli. The compound is widely used with X-Gal plates to screen for blue and white colonies. The lac operon system and IPTG have been used in multiple cloning experiments and cellular systems to study gene activity.IPTG 常用于需要诱导 β-半乳糖苷酶活性的克隆实验。它常与 X-Gal 或 Bluo-Gal 结合使用，用于重组细菌菌落的蓝白筛选，这些菌落可以诱导 lac 操纵子在大肠杆菌中的表达。IPTG 与 lacI 阻遏蛋白结合并改变其构象而发挥作用，防止 β-半乳糖苷酶编码基因 lacZ 的抑制。
IPTG, Dioxane-Free (isopropyl-β-thiogalactoside) is an analogue of allolactose that binds specifically to the repressor protein of the lac operon and induces expression of β-galactosidase in Escherichia coli. The compound is widely used with X-Gal plates to screen for blue and white colonies. The lac operon system and IPTG have been used in multiple cloning experiments and cellular systems to study gene activity.
IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.
相关属性
| CAS编号 |
367-93-1 |
| 敏感性 |
对湿度和热敏感 |
| 比旋光度 |
-32° (C=1,H2O) |
| 熔点 |
116-126°C |
| 溶解性 |
溶于water, 最高浓度 (mg/mL): 23.83, 最高浓度(mM): 100 |
| 储存温度 |
2-8°C储存,充氩 |
| Reaxys-RN |
4631 |
| MDL号 |
MFCD00063273 |
| 密度 |
1.37 |
| 分子量 |
238.3 |
| 分子式 |
C9H18O5S |
| EC号 |
206-703-0 |
| 品牌 |
Jinpan |
| PubChem CID |
656894 |
