荧光素酪酰胺
基本信息
| 产品名称 | 荧光素酪酰胺 |
|---|---|
| 英文名称 | Fluorescein Tyramide |
| 别名 | 3'，6'-二羟基-N-(4-羟基苯基乙基)-3-氧代-3H-螺[异苯并呋喃-1,9'-吨] -5(6)-羧酰胺 |
| 英文别名 | 3′,6′-Dihydroxy-N-(4-hydroxyphenylethyl)-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5(6)-carboxamide |
| 规格或纯度 | ≥95%(HPLC) |
| 运输条件 | 超低温冰袋运输 |
| 生化机理 | 绿色荧光试剂广泛用于IHC，ICC，FISH和多色FISH中的酪氨酰胺信号放大（TSA）。HRP催化多个酪酰胺分子的局部沉积（催化的报告分子沉积，CARD），将荧光素酪酰胺与相邻的酪氨酸结合以增强荧光信号。 |
一般描述
波长 Excitation/Emission 492/514 nm (pH 9)
General description：
Green fluorescent fluorescein tyramide conjugates are used for tyramide signal amplification (TSA), a method for high-density labeling of a target protein or nucleic acid in situ.
High-density labeling of a target protein or nucleic acid for enhanced immunofluorescence sensitivity
Especially suited for the detection of low abundance targets
Detection sensitivity of over 100-fold compared to conventional procedures
Enables multiplex multicolor detection, not limited by antibodies from the same host species
application：
Fluorescein tyramide can be used for tyramide signal amplification (TSA) for increasing immunofluorescence sensitivity in multicolor immunocytochemistry (ICC), immunohistochemistry (IHC), or in situ hybridization (ISH).
相关属性
| CAS编号 | 210236-90-1 |
|---|---|
| 敏感性 | 对光线敏感 |
| 溶解性 | 溶于DMSO, 最高浓度 (mg/mL): 11.89, 最高浓度(mM): 24 |
| 储存温度 | 避光,-20°C储存 |
| 分子量 | 495.48 |
| 分子式 | C29H21NO7 |
| 品牌 | Jinpan |
| Smiles | OC1=CC2=C(C3(C4=CC=C(C=C4O2)O)C5=CC=C(C(NCCC6=CC=C(C=C6)O)=O)C=C5C(O3)=O)C=C1.OC7=CC8=C(C9(C%10=CC=C(C=C%10O8)O)C%11=CC(C(NCCC%12=CC=C(C=C%12)O)=O)=CC=C%11C(O9)=O)C=C7 |
| InChi Key | MJQMGAFVBOOBIB-UHFFFAOYSA-N |