细胞增殖检测试剂盒CFSE Cell Division Tracker Kit
货 号：423801
产品规格：1 kit (500 ug CFSE, 500 ul DMSO)
原 产 地：biolegend
参考价格：1470 （参考价格，以实际价格为准）
优惠价格：
细胞增殖检测试剂盒CFSE Cell Division Tracker Kit 423801 1 kit (500 ug CFSE, 500 ul DMSO)
Preparation: The CFSE Cell Division Tracker Kit is composed of lyophilized CFSE and anhydrous DMSO. For reconstitution, bring the kit to room temperature; add 36 µl of DMSO to one vial of CFSE dye until fully dissolved.
Storage & Handling: Store CFSE Cell Division Tracker Kit at -20°C upon receipt. Do not open vials until needed. Once the DMSO is added to the CFSE, use immediately, or store at -20°C in a dry place and protected from light, preferably in a dessicator or in a container with desiccant for no more than one month. The unopened kit is guaranteed for six months.
This product has a shelf-life of 12 months or less. Please use our Expiration Lookup Tool to verify the expiration date of your lot of product.
Application:
ICFC – Quality tested
In vivo cell tracking1 – Reported in the literature
Recommended Usage:
This lot has been tested by flow cytometric analysis of in vitro cell proliferation assay. It can be used at concentrations ranging from 0.5 – 10 µM for cell labeling. It is recommended that the reagent be titrated for optimal performance for each cell type, culturing condition, or application.
Application
Notes:
The molecular weight of CFSE is 557.47. The excitation and emission wavelengths of CFSE-labeled cells are 492 nm and 517 nm, respectively. Each 100 µg vial of CFSE may be reconstituted with 36 µl of anhydrous DMSO to yield a stock concentration of 5 mM.
Materials Provided:
5 vials x 100 µg CFSE
500 µl anhydrous DMSO
CFSE Labeling Procedure:
1. Prepare a single-cell suspension using desired cells.
2. Resuspend the single-cell suspension at 10-100×106/ml of PBS (pre-warmed to room temperature).
3. Add an equal volume of 2X working solution of CFSE to the desired final concentration (e.g., for a final concentration of 5 µM, prepare a 2X working solution by adding 2 µl of a 5 mM stock in 1.0 ml PBS).
4. Immediately mix and incubate in the dark for 10 minutes at 37°C.
5. To quench the staining add 44 ml of ice cold complete RPMI1640 media (containing 10% FCS).
6. Pellet the cells by centrifugation.
7. Wash once with fresh complete media.
8. At this point, CFSE labeled cells are ready for in vitro assay set up or adoptive transfer experiment.
Application
References:
1. Parish CR, et al. 2009. Curr. Protoc. Immunol. Unit4.9.
2. Miller MJ, et al. 2002. Science 296:1869.
3. Tai LH, 2013. Clin Cancer Res. 19:5104. PubMed
4. Svajger U, et al. 2014. J Lukoc Biol. 95:33. PubMed
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细胞增殖分析对于 研究的生长和化非常重要，检测通基细胞增殖分析对于 研究的生长和化非常重要，检测通基细胞增殖分析对于 研究的生长和化非常重要，检测通基细胞增殖分析对于 研究的生长和化非常重要，检测通基细胞增殖分析对于 研究的生长和化非常重要，检测通基细胞增殖分析对于 研究的生长和化非常重要，检测通基细胞增殖分析对于 研究的生长和化非常重要，检测通基细胞增殖分析对于 研究的生长和化非常重要，检测通基细胞增殖分析对于 研究的生长和化非常重要，检测通基DNA DNA的含量或者细胞代谢参数 的含量或者细胞代谢参数 的含量或者细胞代谢参数 的含量或者细胞代谢参数 。 CFSECFSECFSECFSE方法是一种通过染料稀释检测细胞增殖的， 方法是一种通过染料稀释检测细胞增殖的， 方法是一种通过染料稀释检测细胞增殖的， 方法是一种通过染料稀释检测细胞增殖的， Brdu Brdu 方法是一种基于 方法是一种基于 Brdu Brdu 能够嵌入到新合成的 能够嵌入到新合成的 能够嵌入到新合成的 DNA DNA检测细胞 增殖的方法。 增殖的方法。
货号 产品名称 规格 促销价
422701 CFSE 500 ug 1095
423801
CFSE Cell Division Tracker Kit 1 kit 1470
423401 Bromodeoxyuridine (BrdU) 25 mg 823
339808 APC anti-BrdU 100 tests 2853
339811/339812 PE anti-BrdU 25 tests/100 tests 1283/2788
317902 Purified anti-BrdU 100 ug 1250
产品详细信息
细胞增殖检测试剂盒CFSE Cell Division Tracker Kit 423801 1 kit (500 ug CFSE, 500 ul DMSO)
Preparation: The CFSE Cell Division Tracker Kit is composed of lyophilized CFSE and anhydrous DMSO. For reconstitution, bring the kit to room temperature; add 36 µl of DMSO to one vial of CFSE dye until fully dissolved.
Storage & Handling: Store CFSE Cell Division Tracker Kit at -20°C upon receipt. Do not open vials until needed. Once the DMSO is added to the CFSE, use immediately, or store at -20°C in a dry place and protected from light, preferably in a dessicator or in a container with desiccant for no more than one month. The unopened kit is guaranteed for six months.
This product has a shelf-life of 12 months or less. Please use our Expiration Lookup Tool to verify the expiration date of your lot of product.
Application:
ICFC – Quality tested
In vivo cell tracking1 – Reported in the literature
Recommended Usage:
This lot has been tested by flow cytometric analysis of in vitro cell proliferation assay. It can be used at concentrations ranging from 0.5 – 10 µM for cell labeling. It is recommended that the reagent be titrated for optimal performance for each cell type, culturing condition, or application.
Application
Notes:
The molecular weight of CFSE is 557.47. The excitation and emission wavelengths of CFSE-labeled cells are 492 nm and 517 nm, respectively. Each 100 µg vial of CFSE may be reconstituted with 36 µl of anhydrous DMSO to yield a stock concentration of 5 mM.
Materials Provided:
5 vials x 100 µg CFSE
500 µl anhydrous DMSO
CFSE Labeling Procedure:
1. Prepare a single-cell suspension using desired cells.
2. Resuspend the single-cell suspension at 10-100×106/ml of PBS (pre-warmed to room temperature).
3. Add an equal volume of 2X working solution of CFSE to the desired final concentration (e.g., for a final concentration of 5 µM, prepare a 2X working solution by adding 2 µl of a 5 mM stock in 1.0 ml PBS).
4. Immediately mix and incubate in the dark for 10 minutes at 37°C.
5. To quench the staining add 44 ml of ice cold complete RPMI1640 media (containing 10% FCS).
6. Pellet the cells by centrifugation.
7. Wash once with fresh complete media.
8. At this point, CFSE labeled cells are ready for in vitro assay set up or adoptive transfer experiment.
Application
References:
1. Parish CR, et al. 2009. Curr. Protoc. Immunol. Unit4.9.
2. Miller MJ, et al. 2002. Science 296:1869.
3. Tai LH, 2013. Clin Cancer Res. 19:5104. PubMed
4. Svajger U, et al. 2014. J Lukoc Biol. 95:33. PubMed
细胞增殖检测 细胞增殖检测 细胞增殖检测 细胞增殖检测
细胞增殖分析对于 研究的生长和化非常重要，检测通基细胞增殖分析对于 研究的生长和化非常重要，检测通基细胞增殖分析对于 研究的生长和化非常重要，检测通基细胞增殖分析对于 研究的生长和化非常重要，检测通基细胞增殖分析对于 研究的生长和化非常重要，检测通基细胞增殖分析对于 研究的生长和化非常重要，检测通基细胞增殖分析对于 研究的生长和化非常重要，检测通基细胞增殖分析对于 研究的生长和化非常重要，检测通基细胞增殖分析对于 研究的生长和化非常重要，检测通基DNA DNA的含量或者细胞代谢参数 的含量或者细胞代谢参数 的含量或者细胞代谢参数 的含量或者细胞代谢参数 。 CFSECFSECFSECFSE方法是一种通过染料稀释检测细胞增殖的， 方法是一种通过染料稀释检测细胞增殖的， 方法是一种通过染料稀释检测细胞增殖的， 方法是一种通过染料稀释检测细胞增殖的， Brdu Brdu 方法是一种基于 方法是一种基于 Brdu Brdu 能够嵌入到新合成的 能够嵌入到新合成的 能够嵌入到新合成的 DNA DNA检测细胞 增殖的方法。 增殖的方法。
货号 产品名称 规格 促销价
422701 CFSE 500 ug 1095
423801
CFSE Cell Division Tracker Kit 1 kit 1470
423401 Bromodeoxyuridine (BrdU) 25 mg 823
339808 APC anti-BrdU 100 tests 2853
339811/339812 PE anti-BrdU 25 tests/100 tests 1283/2788
317902 Purified anti-BrdU 100 ug 1250