强力EB去毒剂, ,EB Eraser,

强力EB去毒剂

有货

强力EB去毒剂, ,EB Eraser,

品牌:Jinpan
EB Eraser

MSDS

质检证书(CoA)

相似产品

货号 (SKU) 包装规格 是否现货 价格 数量
E273086-100T 100T 现货 强力EB去毒剂, ,EB Eraser,  

基本信息

产品名称 强力EB去毒剂
英文名称 EB Eraser
运输条件 冰袋运输

一般描述

产品介绍

组成

保存条件

50次

100次

溶液A

室温

100mL

200mL

去毒剂B

室温

25g

50g

本产品收到后按照上面指示温度存放各成分,12个月内有效。

 

溴化乙啶(EB)Ames 测试结果显示EB容易引起有机体突变。强力EB去毒剂是专用于清除溴化乙锭(EB)污染的产品。它能有效破坏EB的结构,清除EB的致癌性,从而实现清洁EB污染的目的。适用于清除电泳缓冲液、生化溶液和固体表面的EB污染(如实验台、离心机、玻璃器皿、不锈钢制品等)。使用强力EB去毒剂将EB污染物处理后,再丢弃可以保护环境不受EB污染物影响。


产品性能指标

能破坏EB的结构,消除EB的荧光,并使其致突变性降低99.5%以上。


注意事项

1.  溶液A有腐蚀性,并且操作EB过程中为保护您的安全,请戴手套和眼罩操作。

2. 化学试剂配制和处理EB过程中可能有微量刺激有害气体产生,请在通风橱中操作。

3. 没有一种方法可以100%消除EB,因此即使处理后,应该戴手套小心操作,而不应该视为100%安全。有条件者,最好定期检测致突变性,确保处理过程的正确。


操作步骤:(实验前请先阅读注意事项)

1.各种污染溶液处理(100mL EB污染溶液)

1) 确保各种污染溶液中EB浓度不超过0.5mg/mL,如果浓度过高,先用水稀释到符合要求的浓度。

2)  工作液准备:在通风橱,用去离子水将2mL溶液A稀释到终体积20mL备用,将0.42g去毒剂B溶于水并定容到12mL备用。

3) 将上述20mL溶液A工作液和12mL去毒剂B工作液加入到100mL EB污染溶液中,仔细搅拌混匀(确保pH≤3)。

4)室温放置反应24小时,用碳酸氢钠调节pH到5-9。

5)用大量水将反应物冲入水槽废弃。

2.各种固体表面污染处理

1)  工作液准备:在通风橱,在300mL去离子水中加入4.2g去毒剂B,充分溶解后加入20mL溶液A,仔细搅拌混匀(pH大约为1.8)。

2) 确保电器都处于断电状态后,用纸巾浸泡刚准备好的工作液,仔细将污染表面擦拭干净,重复6次,每次换用新的浸泡了工作液的纸巾,最后用浸泡了干净去离子水的纸巾擦拭干净工作液,收集纸巾到一个指定处理用容器中。工作液pH值为1.8,有轻微腐蚀性,不宜用来擦拭耐受力弱的物品,可改用去离子水浸泡的纸巾擦拭。擦拭前可用紫外灯帮助发现污染区,擦拭后帮助确认已经擦拭干净。

3) 将这些污染纸巾浸泡在工作液中至少室温放置一个小时,用碳酸氢钠调节pH到5-9后,液体用大量水冲入水槽,纸巾入垃圾堆。

Product description

Composition

Storage Conditions

50T

100T

Solution A

Room temperature

100mL

200mL

Detox B

Room temperature

25g

50g

After the product is received, store the ingredients at the temperature indicated above, and it will be valid for 12 months.

 

Ethidium bromide (EB) Ames test results show that EB can easily cause mutations in organisms. Powerful EB Detoxifier is a product specially designed to remove ethidium bromide (EB) pollution. It can effectively destroy the structure of EB and remove the carcinogenicity of EB, thereby achieving the purpose of cleaning EB pollution. It is suitable for removing EB contamination of electrophoresis buffer, biochemical solution and solid surface (such as laboratory bench, centrifuge, glassware, stainless steel products, etc.). After using a powerful EB detoxifier to treat EB pollutants, then discarding it can protect the environment from EB pollutants.

Product performance index

It can destroy the structure of EB, eliminate the fluorescence of EB, and reduce its mutagenicity by more than 99.5%.

Precautions

1. Solution A is corrosive, and to protect your safety during the operation of EB, please wear gloves and goggles.

2. There may be a small amount of irritating and harmful gas generated during the chemical reagent preparation and processing of EB, please operate in a fume hood.

3. There is no method that can eliminate EB 100%, so even after handling, you should wear gloves and handle it carefully, not as 100% safe. If conditions permit, it is best to regularly test for mutagenicity to ensure the correctness of the treatment process.

Operation steps: (Please read the notes before experiment)

1. Treatment of various pollution solutions (100mL EB pollution solution)

1) Ensure that the concentration of EB in various polluted solutions does not exceed 0.5mg/mL. If the concentration is too high, first dilute with water to meet the required concentration.

2) Preparation of working solution: In a fume hood, dilute 2 mL of solution A with deionized water to a final volume of 20 mL for use. Dissolve 0.42 g of detoxifier B in water and dilute to 12 mL for use.

3) Add the above 20mL solution A working solution and 12mL detoxifier B working solution to 100mL EB contaminated solution, stir and mix carefully (make sure pH≤3).

4) Allow to react at room temperature for 24 hours, adjust the pH to 5-9 with sodium bicarbonate.

5) Wash the reactants into the water tank with a lot of water for disposal.

2. Various solid surface pollution treatment

1) Preparation of working solution: In a fume hood, add 4.2g of detoxifier B to 300mL of deionized water, add 20mL of solution A after fully dissolving, stir and mix carefully (pH is about 1.8).

2) After ensuring that the electrical appliances are in a power-off state, soak the freshly prepared working fluid with a paper towel, carefully wipe clean the contaminated surface, repeat 6 times, each time change to a new paper towel soaked in the working fluid, and finally soak it clean Wipe clean the working fluid with paper towels of deionized water, and collect the paper towels into a designated processing container. The pH value of the working fluid is 1.8, which is slightly corrosive. It is not suitable for wiping items with weak tolerance. You can use paper towels soaked in deionized water to wipe. Before wiping, a UV lamp can be used to help find the contaminated area. After wiping, it can help confirm that it has been wiped clean.

3) Soak these contaminated paper towels in the working fluid at room temperature for at least one hour. After adjusting the pH to 5-9 with sodium bicarbonate, the liquid is flushed into the sink with plenty of water, and the paper towels are put into the garbage dump.

相关属性

敏感性 对光和湿度敏感
储存温度 2-8°C储存,避光,干燥
品牌 Jinpan