蛋白L,FITC-Labeled Recombinant Protein L 货号:RPL-PF141

蛋白L,FITC-Labeled Recombinant Protein L 货号:RPL-PF141

蛋白L,FITC-Labeled Recombinant Protein L

货  号:RPL-PF141

产品规格:200ug

原  产  地:

参考价格:1200 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

FITC-Labeled Recombinant Protein L

蛋白L,FITC-Labeled Recombinant Protein L

分子别名(Synonym)

RPL,Protein L

表达区间及表达系统(Source)

FITC-Labeled Recombinant Protein L (Cat. No. RPL-PF141) is expressed from human HEK293 cells. It is the FITC labeled form of Recombinant Protein L (Cat. No. RPL-P3141).

Request for sequence

蛋白结构(Molecular Characterization)

蛋白L,FITC-Labeled Recombinant Protein L 货号:RPL-PF141

FITC-Labeled Recombinant Protein L is fused with a polyhistidine tag at the N-terminus. The reducing (R) protein migrates as 40-45 kDa in SDS-PAGE.

Conjugate

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

标记(Labeling)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.

蛋白标记度(FITC:Protein Ratio)

The FITC to protein molar ratio is 5-7.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

活性(Bioactivity)-ELISA

蛋白L,FITC-Labeled Recombinant Protein L 货号:RPL-PF141

Immobilized Yervoy Ipilumimab at 10 μg/mL (100 μL/well) can bind FITC-Labeled Recombinant Protein L (Cat. No. RPL-PF141) with a linear range of 20-156 ng/mL (Ex.488 nm/Em.535 nm) (QC tested).

产品详细信息

FITC-Labeled Recombinant Protein L

蛋白L,FITC-Labeled Recombinant Protein L

分子别名(Synonym)

RPL,Protein L

表达区间及表达系统(Source)

FITC-Labeled Recombinant Protein L (Cat. No. RPL-PF141) is expressed from human HEK293 cells. It is the FITC labeled form of Recombinant Protein L (Cat. No. RPL-P3141).

Request for sequence

蛋白结构(Molecular Characterization)

蛋白L,FITC-Labeled Recombinant Protein L 货号:RPL-PF141

FITC-Labeled Recombinant Protein L is fused with a polyhistidine tag at the N-terminus. The reducing (R) protein migrates as 40-45 kDa in SDS-PAGE.

Conjugate

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

标记(Labeling)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.

蛋白标记度(FITC:Protein Ratio)

The FITC to protein molar ratio is 5-7.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

活性(Bioactivity)-ELISA

蛋白L,FITC-Labeled Recombinant Protein L 货号:RPL-PF141

Immobilized Yervoy Ipilumimab at 10 μg/mL (100 μL/well) can bind FITC-Labeled Recombinant Protein L (Cat. No. RPL-PF141) with a linear range of 20-156 ng/mL (Ex.488 nm/Em.535 nm) (QC tested).

FITC标记细胞凋亡检测试剂盒 货号:KT40003

FITC标记细胞凋亡检测试剂盒 货号:KT40003

FITC标记细胞凋亡检测试剂盒

货  号:KT40003

产品规格:100T

原  产  地:abgent

参考价格:1600 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

FITC标记细胞凋亡检测试剂盒  KT40003  100T

FITC Annexin V Apoptosis  Detection Kit
Catalog #KT40003

25tests¥ 670.00

100tests¥ 1,600.00

产品详细信息

FITC标记细胞凋亡检测试剂盒  KT40003  100T

FITC Annexin V Apoptosis  Detection Kit
Catalog #KT40003

25tests¥ 670.00

100tests¥ 1,600.00

FITC anti-mouse CD45 货号:103108

FITC anti-mouse CD45 货号:103108

FITC anti-mouse CD45

货  号:103108

产品规格:500ug

原  产  地:biolegend

参考价格:2470 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

FITC anti-mouse CD45  103108  500ug

Clone:     30-F11 (See other available formats)
Isotype:     Rat IgG2b, κ
Isotype Control:    FITC Rat IgG2b, κ Isotype Ctrl
Reactivity:     Mouse
Immunogen:     Mouse thymus or spleen
Formulation:     Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation:     The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration:     0.5 mg/ml
Storage & Handling:     The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:     

FC – Quality tested
Application
References:     

1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
8. Liu F, et al. 2012. Blood. 119:3295. PubMed
9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
10. Ito K, et al. 2014. PNAS. 111:3080. PubMed
11. Abdala-Valencia H, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:482. PubMed
12. Zhang K, et al. 2014. PLoS Pathog. 10:1004385. PubMed

产品详细信息

FITC anti-mouse CD45  103108  500ug

Clone:     30-F11 (See other available formats)
Isotype:     Rat IgG2b, κ
Isotype Control:    FITC Rat IgG2b, κ Isotype Ctrl
Reactivity:     Mouse
Immunogen:     Mouse thymus or spleen
Formulation:     Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation:     The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration:     0.5 mg/ml
Storage & Handling:     The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:     

FC – Quality tested
Application
References:     

1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
8. Liu F, et al. 2012. Blood. 119:3295. PubMed
9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
10. Ito K, et al. 2014. PNAS. 111:3080. PubMed
11. Abdala-Valencia H, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:482. PubMed
12. Zhang K, et al. 2014. PLoS Pathog. 10:1004385. PubMed

FITC anti-mouse CD3e 货号:100306

FITC anti-mouse CD3e 货号:100306

FITC anti-mouse CD3e

货  号:100306

产品规格:500 ug

原  产  地:biolegend

参考价格:2470元 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

FITC anti-mouse CD3e  100306  biolegend  细胞分群流式抗体

Clone:     145-2C11 (See other available formats)
Isotype:     Armenian Hamster IgG
Isotype Control:    FITC Armenian Hamster IgG Isotype Ctrl
Reactivity:     Mouse
Immunogen:     H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Formulation:     Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation:     The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration:     0.5 mg/ml
Storage & Handling:     The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:     

FC – Quality tested
Application
Notes:     

The 145-2C11 antibody is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vivo T cell depletions. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6, in vitro and in vivo stimulation of T cells1,2,7,12,16, immunofluorescent staining5, and in vivo T cell depletions8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application
References:     

1. Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activ)
2. Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. 3.12.1. (Activ)
3. Duke RC, et al. 1995. Current Protocols in Immunology. 3.17.1.
4. Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB)
5. Payer E, et al. 1991. J. Immunol. 146:2536. (IF)
6. Jacobs H, et al. 1994. Eur. J. Immunol. 24:934. (CMCD)
7. Vossen ACTM, et al. 1995. Eur. J. Immunol. 25:1492. (Activ)
8. Henrickson M, et al. 1995. Transplantation 60:828. (Deplete)
9. Kinnaert P, et al. 1996. Transpl. Int. 9:386. (Deplete)
10. Han WR, et al. 1999. Transpl. Immunol. 7:207. (Deplete)
11. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block)
12. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Activ)

产品详细信息

FITC anti-mouse CD3e  100306  biolegend  细胞分群流式抗体

Clone:     145-2C11 (See other available formats)
Isotype:     Armenian Hamster IgG
Isotype Control:    FITC Armenian Hamster IgG Isotype Ctrl
Reactivity:     Mouse
Immunogen:     H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Formulation:     Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation:     The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration:     0.5 mg/ml
Storage & Handling:     The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:     

FC – Quality tested
Application
Notes:     

The 145-2C11 antibody is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vivo T cell depletions. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6, in vitro and in vivo stimulation of T cells1,2,7,12,16, immunofluorescent staining5, and in vivo T cell depletions8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application
References:     

1. Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activ)
2. Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. 3.12.1. (Activ)
3. Duke RC, et al. 1995. Current Protocols in Immunology. 3.17.1.
4. Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB)
5. Payer E, et al. 1991. J. Immunol. 146:2536. (IF)
6. Jacobs H, et al. 1994. Eur. J. Immunol. 24:934. (CMCD)
7. Vossen ACTM, et al. 1995. Eur. J. Immunol. 25:1492. (Activ)
8. Henrickson M, et al. 1995. Transplantation 60:828. (Deplete)
9. Kinnaert P, et al. 1996. Transpl. Int. 9:386. (Deplete)
10. Han WR, et al. 1999. Transpl. Immunol. 7:207. (Deplete)
11. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block)
12. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Activ)

FITC anti-human CD45 Antibody 货号:304006

FITC anti-human CD45 Antibody 货号:304006

FITC anti-human CD45 Antibody

货  号:304006

产品规格:100 tests

原  产  地:biolegend

参考价格:1690 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

FITC anti-human CD45 Antibody  304006  100 tests 细胞分群流式抗体

Clone:     HI30 (See other available formats)
Isotype:     Mouse IgG1, κ
Isotype Control:    FITC Mouse IgG1, κ Isotype Ctrl
Workshop
Number:     IV N816
Reactivity:     Human, Cross-Reactivity: Chimpanzee
Formulation:     Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation:     The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Storage & Handling:     The CD45 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
2. Kishihara K, et al. 1993. Cell 74:143.
3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
4. Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
5. Nagano M, et al. 2007. Blood 110:151.
6. Jiang Q, et al. 2008. Blood 112:2858. PubMed
7. Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
9. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
10. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
11. Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)

产品详细信息

FITC anti-human CD45 Antibody  304006  100 tests 细胞分群流式抗体

Clone:     HI30 (See other available formats)
Isotype:     Mouse IgG1, κ
Isotype Control:    FITC Mouse IgG1, κ Isotype Ctrl
Workshop
Number:     IV N816
Reactivity:     Human, Cross-Reactivity: Chimpanzee
Formulation:     Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation:     The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Storage & Handling:     The CD45 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
2. Kishihara K, et al. 1993. Cell 74:143.
3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
4. Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
5. Nagano M, et al. 2007. Blood 110:151.
6. Jiang Q, et al. 2008. Blood 112:2858. PubMed
7. Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
9. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
10. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
11. Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)

FITC anti-human CD3 货号:300406

FITC anti-human CD3 货号:300406

FITC anti-human CD3

货  号:300406

产品规格:100 tests

原  产  地:USA

参考价格:1235 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

300406 FITC anti-human CD3 100 tests

Clone UCHT1
Isotype Mouse IgG1, κ
Reactivity Human, Cross-Reactivity: Chimpanzee
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Applications

FC – Quality tested

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, and Western blotting9. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 300414). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300438) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC)
9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)

Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Storage The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

产品详细信息

300406 FITC anti-human CD3 100 tests

Clone UCHT1
Isotype Mouse IgG1, κ
Reactivity Human, Cross-Reactivity: Chimpanzee
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Applications

FC – Quality tested

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, and Western blotting9. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 300414). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300438) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC)
9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)

Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Storage The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

FITC Annexin V Apoptosis Detection Kit with 7-AAD 货号:640922

FITC Annexin V Apoptosis Detection Kit with 7-AAD 货号:640922

FITC Annexin V Apoptosis Detection Kit with 7-AAD

货  号:640922

产品规格:100T

原  产  地:USA

参考价格:2795 (参考价格,以实际价格为准)

优惠价格:2375

产品详细信息

FITC Annexin V Apoptosis Detection Kit with 7-AAD 640922  100T

Reactivity:     All mammalian species
Storage & Handling:     Store between 2°C and 8°C. Do not freeze.

Caution: 7-AAD is a potential carcinogen. It is recommended that the user wear protective clothing, gloves, and eye/face protection in order to avoid contact with skin and eyes.
Recommended Usage:     

Staining Procedure:
1. Wash cells twice with cold BioLegend’s Cell Staining Buffer, and then resuspend cells in Annexin V Binding Buffer at a concentration of 0.25-1.0 x 107 cells/ml.
2. Transfer 100 µl of cell suspension in a 5 ml test tube.
3. Add 5 µl of FITC Annexin V.
4. Add 5 µl of 7-AAD Viability Staining Solution.
5. Gently vortex the cells and incubate for 15 min at room temperature (25°C) in the dark.
6. Add 400 µl of Annexin V Binding Buffer to each tube. Analyze by flow cytometry with proper machine settings.

 

产品详细信息

FITC Annexin V Apoptosis Detection Kit with 7-AAD 640922  100T

Reactivity:     All mammalian species
Storage & Handling:     Store between 2°C and 8°C. Do not freeze.

Caution: 7-AAD is a potential carcinogen. It is recommended that the user wear protective clothing, gloves, and eye/face protection in order to avoid contact with skin and eyes.
Recommended Usage:     

Staining Procedure:
1. Wash cells twice with cold BioLegend’s Cell Staining Buffer, and then resuspend cells in Annexin V Binding Buffer at a concentration of 0.25-1.0 x 107 cells/ml.
2. Transfer 100 µl of cell suspension in a 5 ml test tube.
3. Add 5 µl of FITC Annexin V.
4. Add 5 µl of 7-AAD Viability Staining Solution.
5. Gently vortex the cells and incubate for 15 min at room temperature (25°C) in the dark.
6. Add 400 µl of Annexin V Binding Buffer to each tube. Analyze by flow cytometry with proper machine settings.

 

FITC Annexin V Apoptosis Detection Kit with PI 货号:640914

FITC Annexin V Apoptosis Detection Kit with PI 货号:640914

FITC Annexin V Apoptosis Detection Kit with PI

货  号:640914

产品规格:100T

原  产  地:USA

参考价格:2795 (参考价格,以实际价格为准)

优惠价格:2375

产品详细信息
FITC Annexin V Apoptosis Detection Kit with PI
Reactivity:All mammalian speciesStorage & Handling:Store between 2°C and 8°C.Do not freeze.

Caution:Propidium Iodide Solution is toxigenic and mutagenic; handle with care.Application:FC -Quality testedRecommended Usage:Staining Procedure:
1. Wash cells twice with cold BioLegend’s Cell Staining Buffer, and then resuspend cells in Annexin V Binding Buffer at a concentration of 0.25-1.0 x 107cells/ml.
2. Transfer 100 µl of cell suspension in a 5 ml test tube.
3. Add 5 µl of FITC Annexin V.
4. Add 10 µl of Propidium Iodide Solution.
5. Gently vortex the cells and incubate for 15 min at room temperature (25°C) in the dark.
6. Add 400 µl of Annexin V Binding Buffer to each tube. Analyze by flow cytometry with proper machine settings.COA:Enter Lot#:  FITC Annexin V Apoptosis Detection Kit with PI 货号:640914Application
Notes:Materials Provided:
0.5 ml of FITC Annexin V
1 ml of Propidium Iodide Solution
50 ml of Annexin V Binding Buffer

Materials Not Included:
Cell Staining Buffer (Cat. No. 420201)Application
References:1. Ranhanathan P,et al.2013.J AM Soc Nephrol.PubMed Publication Library
FITC Annexin V Apoptosis Detection Kit with PI 货号:640914

Human T-cell leukemia cell line, Jurkat, treated (top) or non-treated (bottom) with BioLegend’s LEAF™ purified anti-CD95 (clone EOS9.1) mAb (Cat. No. 305704) for 4 hours, then stained with FITC Annexin V Apoptosis Detection Kit with PI (Cat. No. 640914)
FITC Annexin V Apoptosis Detection Kit with PI 货号:640914

Compare all formats

Description:BioLegend’s FITC Annexin V Apoptosis Detection Kit with PI has been specifically designed for the identification of apoptotic and necrotic cells.

Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer is recommended for use with Annexin V staining. Annexin V binding alone cannot differentiate between apoptotic and necrotic cells. To help distinguish between the necrotic and apoptotic cells we recommend use of our Propidium Iodide Solution (PI). Early apoptotic cells will exclude PI, while late stage apoptotic cells and necrotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA.

Propidium iodide is a fluorescent dye that binds to DNA. When excited by 488 nm laser light, it can be detected with 562-588 nm band pass filter. It is commonly used in evaluation of cell viability or DNA content in cell cycle analysis by flow cytometry.

Other Names:Annexin A5 Apoptosis Detection Kit

产品详细信息

FITC Annexin V Apoptosis Detection Kit with PI
Reactivity:All mammalian speciesStorage & Handling:Store between 2°C and 8°C.Do not freeze.

Caution:Propidium Iodide Solution is toxigenic and mutagenic; handle with care.Application:FC -Quality testedRecommended Usage:Staining Procedure:
1. Wash cells twice with cold BioLegend’s Cell Staining Buffer, and then resuspend cells in Annexin V Binding Buffer at a concentration of 0.25-1.0 x 107cells/ml.
2. Transfer 100 µl of cell suspension in a 5 ml test tube.
3. Add 5 µl of FITC Annexin V.
4. Add 10 µl of Propidium Iodide Solution.
5. Gently vortex the cells and incubate for 15 min at room temperature (25°C) in the dark.
6. Add 400 µl of Annexin V Binding Buffer to each tube. Analyze by flow cytometry with proper machine settings.COA:Enter Lot#:  FITC Annexin V Apoptosis Detection Kit with PI 货号:640914Application
Notes:Materials Provided:
0.5 ml of FITC Annexin V
1 ml of Propidium Iodide Solution
50 ml of Annexin V Binding Buffer

Materials Not Included:
Cell Staining Buffer (Cat. No. 420201)Application
References:1. Ranhanathan P,et al.2013.J AM Soc Nephrol.PubMed Publication Library
FITC Annexin V Apoptosis Detection Kit with PI 货号:640914

Human T-cell leukemia cell line, Jurkat, treated (top) or non-treated (bottom) with BioLegend’s LEAF™ purified anti-CD95 (clone EOS9.1) mAb (Cat. No. 305704) for 4 hours, then stained with FITC Annexin V Apoptosis Detection Kit with PI (Cat. No. 640914)
FITC Annexin V Apoptosis Detection Kit with PI 货号:640914

Compare all formats

Description:BioLegend’s FITC Annexin V Apoptosis Detection Kit with PI has been specifically designed for the identification of apoptotic and necrotic cells.

Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer is recommended for use with Annexin V staining. Annexin V binding alone cannot differentiate between apoptotic and necrotic cells. To help distinguish between the necrotic and apoptotic cells we recommend use of our Propidium Iodide Solution (PI). Early apoptotic cells will exclude PI, while late stage apoptotic cells and necrotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA.

Propidium iodide is a fluorescent dye that binds to DNA. When excited by 488 nm laser light, it can be detected with 562-588 nm band pass filter. It is commonly used in evaluation of cell viability or DNA content in cell cycle analysis by flow cytometry.

Other Names:Annexin A5 Apoptosis Detection Kit

FITC Annexin V 凋亡试剂盒 货号:640905

FITC Annexin V 凋亡试剂盒 货号:640905

FITC Annexin V 凋亡试剂盒

货  号:640905

产品规格:25T

原  产  地:USA

参考价格:910 (参考价格,以实际价格为准)

优惠价格:773

产品详细信息

FITC Annexin V 凋亡试剂盒 640905  25T

Reactivity:     All mammalian species
Formulation:     Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation:     The purified protein was conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Storage & Handling:     The Annexin V solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:     

FC – Quality tested
Recommended Usage:     

Each lot of this product is quality control tested by staining of apoptotic cells and analyzing by flow cytometry. The suggested use of this reagent is 5 µl per 1X105 cells in a 100 µl volume of Annexin V Binding Buffer (cat. no. 422201). It is recommended that the reagent be titrated for optimal performance for each application.
Excitation
Laser:     Blue Laser (488 nm)
Application
Notes:     

Annexin V Staining
1. Wash cells twice with cold BioLegend cell staining buffer (cat # 420201) and then resuspend cells in Annexin V Binding Buffer (cat # 422201) at a concentration of 1x10e6 cells/ml.
2. Transfer 100 µl of cell suspension in 5 ml test tube.
3. Add 5 µl of FITC Annexin V.
4. Add 10 µl of PI solution (cat # 421301) or 7-AAD (cat # 420403/420404).
5. Gently vortex the cells and incubate for 15 min at RT (25 °C) in the dark.
6. Add 400 µl of Annexin V Binding Buffer (cat # 422201) to each tube. Analyze by flow cytometry.
Application
References:     

1. Koopman G, et al. 1994. Blood 84:1415.
2. Vermes I, et al. 1995. J. Immunol. Methods 184:39.
3. Dachary-Prigent J, et al. 1993. Blood 81:2554.
4. Sekine C, et al. 2009. Int Immunol. PubMed
5. Grujic M, et al. 2010. J. Immunol. 185:1730. PubMed
6. Mulik RS, et al. 2010. Int J Pharm. 398:190. PubMed
7. Gupta A, et al. 2011. Leuk Res. 35:1498. PubMed
8. Speth C,et al. 2013. J Infect Dis. 207:823.  PubMed
9. Wang Q, et al. 2013. Genes Dev. 27:615. PubMed
10. Gill K, et al. 2013. Biocheim Biophys Acta. 830:2763. PubMed
11. Juel HB, et al. 2013. PLoS One. 8:64619. PubMed
12. Hellmann J, et al. 2013. J. Immunol. 191:1383. PubMed

产品详细信息

FITC Annexin V 凋亡试剂盒 640905  25T

Reactivity:     All mammalian species
Formulation:     Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation:     The purified protein was conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Storage & Handling:     The Annexin V solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:     

FC – Quality tested
Recommended Usage:     

Each lot of this product is quality control tested by staining of apoptotic cells and analyzing by flow cytometry. The suggested use of this reagent is 5 µl per 1X105 cells in a 100 µl volume of Annexin V Binding Buffer (cat. no. 422201). It is recommended that the reagent be titrated for optimal performance for each application.
Excitation
Laser:     Blue Laser (488 nm)
Application
Notes:     

Annexin V Staining
1. Wash cells twice with cold BioLegend cell staining buffer (cat # 420201) and then resuspend cells in Annexin V Binding Buffer (cat # 422201) at a concentration of 1x10e6 cells/ml.
2. Transfer 100 µl of cell suspension in 5 ml test tube.
3. Add 5 µl of FITC Annexin V.
4. Add 10 µl of PI solution (cat # 421301) or 7-AAD (cat # 420403/420404).
5. Gently vortex the cells and incubate for 15 min at RT (25 °C) in the dark.
6. Add 400 µl of Annexin V Binding Buffer (cat # 422201) to each tube. Analyze by flow cytometry.
Application
References:     

1. Koopman G, et al. 1994. Blood 84:1415.
2. Vermes I, et al. 1995. J. Immunol. Methods 184:39.
3. Dachary-Prigent J, et al. 1993. Blood 81:2554.
4. Sekine C, et al. 2009. Int Immunol. PubMed
5. Grujic M, et al. 2010. J. Immunol. 185:1730. PubMed
6. Mulik RS, et al. 2010. Int J Pharm. 398:190. PubMed
7. Gupta A, et al. 2011. Leuk Res. 35:1498. PubMed
8. Speth C,et al. 2013. J Infect Dis. 207:823.  PubMed
9. Wang Q, et al. 2013. Genes Dev. 27:615. PubMed
10. Gill K, et al. 2013. Biocheim Biophys Acta. 830:2763. PubMed
11. Juel HB, et al. 2013. PLoS One. 8:64619. PubMed
12. Hellmann J, et al. 2013. J. Immunol. 191:1383. PubMed

Anti-CD27 FITC 货号:11-0271-81

Anti-CD27 FITC 货号:11-0271-81

Anti-CD27 FITC

货  号:11-0271-81

产品规格:50ug

原  产  地:USA

参考价格:948 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

Anti-CD27 FITC

11-0271-81
50ug
USA

The LG.7F9 monoclonal antibody reacts with mouse CD27, alymphocyte-specific member of

the TNFR superfamily. CD27 is expressed by virtually all mature T cellsand by a subpopulation
of B cells, mainly memory B cells. In mouse, CD27 has been found onnearly all thymocytes
excluding a population of CD46-CD8- precursors. CD27 binds to CD70 and,through this interaction,
plays an important role in T cell-B cell interaction. It has beenreported that triggering CD27
plays an important role in the maturation of CD4+ and CD8+ effectorcells. LG.7F9 cross-reacts
with human and rat CD27.

产品详细信息

Anti-CD27 FITC

11-0271-81
50ug
USA

The LG.7F9 monoclonal antibody reacts with mouse CD27, alymphocyte-specific member of

the TNFR superfamily. CD27 is expressed by virtually all mature T cellsand by a subpopulation
of B cells, mainly memory B cells. In mouse, CD27 has been found onnearly all thymocytes
excluding a population of CD46-CD8- precursors. CD27 binds to CD70 and,through this interaction,
plays an important role in T cell-B cell interaction. It has beenreported that triggering CD27
plays an important role in the maturation of CD4+ and CD8+ effectorcells. LG.7F9 cross-reacts
with human and rat CD27.