碘化丙啶

产品介绍

1. 外观：红棕色粉末

2. 纯度：≥98%（HPLC）

3. 产品参数: Ex(nm) 535    Em(nm) 617   Solvent: Water

产品描述

PI是一种可对DNA染色的细胞核染色试剂，与EB一样PI嵌入双链DNA后释放红色荧光。常用于细胞凋亡(apoptosis)或细胞坏死(necrosis)的检测，常用于流式细胞仪分析。尽管PI不能通过活细胞膜，但却能穿过破损的细胞膜而对核染色。PI经常被用来与Calcein, AM、Hoechst 33258或Hoechst 33342等一起使用，能同时对活细胞和死细胞染色。PI-DNA复合物的激发和发射波长分别为535nm和 615nm。

染色过程

（1） 用PBS或适当的缓冲液制备10～50μM的PI溶液。
（2） 将1/10培养基体积的PI溶液加入到细胞培养基中（也可以用1/10浓度的 PI缓冲液代替培养基）。

（3） 在37℃培养细胞10～20分钟。
（4） 用PBS或合适的缓冲液洗涤细胞两次。
（5） 用535nm激发波长，615nm发射波长的滤光器的荧光显微镜观察细胞。

储存条件：-20℃干燥避光保存，有效期一年。

注意事项

MSDS显示碘化丙啶PI与EB一样对生殖细胞致突变性，是致癌物质，请必须严格注意防护！

碘化丙啶PI是Germ cell mutagenicity生殖细胞突变物质；In vitro tests showed mutagenic effects体外实验显示有致突变性！

Product introduction

1. Appearance: reddish brown powder

2. Purity: ≥98% (HPLC)

3. Product parameters: Ex(nm) 535    Em(nm) 617    Solvent: Water

Product description

PI is a nuclear staining reagent that can stain DNA. Like EB, PI intercalates into double-stranded DNA and releases red fluorescence. It is often used for the detection of apoptosis or necrosis, and is often used for flow cytometry analysis. Although PI cannot pass through living cell membranes, it can pass through damaged cell membranes to stain nuclei. PI is often used with Calcein, AM, Hoechst 33258 or Hoechst 33342, etc. It can stain live and dead cells at the same time. The excitation and emission wavelengths of the PI-DNA complex are 535nm and 615nm, respectively.

Dyeing process

(1) Prepare 10-50μM PI solution with PBS or appropriate buffer.

(2) Add 1/10 of the volume of the medium to the cell culture medium (1/10 concentration of PI buffer can also be used to replace the medium).

(3) Incubate the cells at 37°C for 10-20 minutes.

(4) Wash the cells twice with PBS or a suitable buffer.

(5) Observe the cells with a fluorescence microscope with a 535nm excitation wavelength and a 615nm emission wavelength filter.

Storage conditions: Store at -20°C dry and dark, valid for one year.
Precautions

MSDS shows that propidium iodide PI is the same as EB mutagenic to germ cells and is a carcinogen. Please take strict care to protect it!

Propidium iodide PI is a Germ cell mutagenicity germ cell mutant; In vitro tests showed mutagenic effects!