磷脂酰肌醇激酶PI3K P110 alpha抗体
货 号：4249
产品规格： 0.1ml
原 产 地：USA
参考价格：3100 （参考价格，以实际价格为准）
优惠价格：
宿主： Goat，Rabbit，Mouse
抗体英文名： Anti-PI3K P110 alpha(Phosphatidylinositol 3 kinase catalytic alpha)
品牌： CST
克隆性： 否
供应商： 上海金畔生物科技有限公司
应用范围： IHC/WB
适应物种： 人，大鼠，小鼠
保质期： 一年
抗原来源： Goat，Rabbit，Mouse
保存条件： 低温
抗体名： PI3K P110 alpha
规格： 0.1ml
Anti-PI3K P110 alpha,磷脂酰肌醇激酶PI3K P110 alpha抗体 7折促销
Specificity / Sensitivity
PI3 Kinase p110α (C73F8) Rabbit mAb detects endogenous levels of total PI3K p110α protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Asp520 of the sequence of human PI3K p110α.
Western Blotting
Western blot analysis of extracts from HeLa cells and neonatal mouse brain using PI3 Kinase p110α (C73F8) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PI3 Kinase p110α (C73F8) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PI3 Kinase p110α (C73F8) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Background
Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and research studies have shown that the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4).
- Cantley, L.C. (2002) Science 296, 1655-7.
- Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41.
- Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80.
- Stoyanov, B. et al. (1995) Science 269, 690-3.
Application References
- Popkie, A.P. et al. (2010) J Biol Chem 285, 41337-47. Applications: Western Blotting
- Yoshioka, K. et al. (2012) Nat Med 18, 1560-9. Applications: Western Blotting
- Biswas, K. et al. (2013) J Biol Chem 288, 2325-39. Applications: Western Blotting
产品详细信息 数量： 3周-7折促销
宿主： Goat，Rabbit，Mouse
抗体英文名： Anti-PI3K P110 alpha(Phosphatidylinositol 3 kinase catalytic alpha)
品牌： CST
克隆性： 否
供应商： 上海金畔生物科技有限公司
应用范围： IHC/WB
适应物种： 人，大鼠，小鼠
保质期： 一年
抗原来源： Goat，Rabbit，Mouse
保存条件： 低温
抗体名： PI3K P110 alpha
规格： 0.1ml
Anti-PI3K P110 alpha,磷脂酰肌醇激酶PI3K P110 alpha抗体 7折促销
Specificity / Sensitivity
PI3 Kinase p110α (C73F8) Rabbit mAb detects endogenous levels of total PI3K p110α protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Asp520 of the sequence of human PI3K p110α.
Western Blotting
Western blot analysis of extracts from HeLa cells and neonatal mouse brain using PI3 Kinase p110α (C73F8) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PI3 Kinase p110α (C73F8) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PI3 Kinase p110α (C73F8) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Background
Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and research studies have shown that the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4).
- Cantley, L.C. (2002) Science 296, 1655-7.
- Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41.
- Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80.
- Stoyanov, B. et al. (1995) Science 269, 690-3.
Application References
- Popkie, A.P. et al. (2010) J Biol Chem 285, 41337-47. Applications: Western Blotting
- Yoshioka, K. et al. (2012) Nat Med 18, 1560-9. Applications: Western Blotting
- Biswas, K. et al. (2013) J Biol Chem 288, 2325-39. Applications: Western Blotting