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RAB11 Polyclonal Antibody

发表于2017年5月3日由上海金畔生物科技有限公司

RAB11 Polyclonal Antibody for Western Blot, IF, ICC, IHC (P), Flow, IP, ELISA and IM

RAB11 Polyclonal Antibody RAB11 Polyclonal Antibody RAB11 Polyclonal Antibody RAB11 Polyclonal Antibody RAB11 Polyclonal Antibody RAB11 Polyclonal Antibody RAB11 Polyclonal Antibody RAB11 Polyclonal Antibody

RAB11 Polyclonal Antibody

RAB11 Antibody (71-5300) in IF

Immunofluorescence analysis of Rab11 was done on 70% confluent log phase U2OS cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Rab11 Rabbit Polyclonal Antibody (715300) at 2 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing cytoplasmic localization. Panel e shows no primary antibody control. The images were captured at 20X magnification.

Performance guaranteed

Purchase with confidence, knowing that we stand behind the performance of our antibodies with the InvitrogenTM antibody performance guarantee. If an InvitrogenTM antibody doesn’t perform in your experiment as described on our website or datasheet, we’ll replace the product at no cost to you, or if you prefer, we’ll provide you with a credit for a future purchase.* Catalog#: 71-5300 Invitrogen RAB11 Polyclonal Antibody

Details
Tested species reactivity Dog, Human, Mouse, Rabbit, Rat
Published species reactivity Dog, Rat, Hamster, Human, Mouse, Not Applicable, Rhesus monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Synthetic peptide derived from the C-terminal hypervariable region of the human Rab11a sequence. This peptide shares 10 of 17 residues with the related Rab11b protein.
Conjugate Unconjugated
Form Liquid
Concentration 0.25 mg/ml
Purification Antigen affinity chromatography
Storage buffer PBS, pH 7.4
Contains 0.1% sodium azide
Storage Conditions -20°C

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原文地址:http://www.thermofisher.com/antibody/product/RAB11-Antibody-Polyclonal/71-5300

此条目是由上海金畔生物科技有限公司发表在未分类分类目录的。将固定链接加入收藏夹。
沪ICP备14053477号-16 上海金畔生物科技有限公司