6-FAM亚磷酰胺

6-FAM亚磷酰胺是寡核苷酸标记。有几种用荧光素标记寡核苷酸的方法。根据光谱要求，标签的选择进一步多样化。衍生自单一异构体6-羧基荧光素，5′-六氯荧光素-CE亚磷酰胺（HEX）和5′-四氯荧光素-CE亚磷酰胺（TET）的5′-荧光素-CE亚磷酰胺均可用于在5处高效标记寡核苷酸’-结尾。可以使用3′-荧光素CPG对荧光素标记寡核苷酸的3′-末端。当使用这些载体中的任何一种时，标准的裂解和氢氧化铵的脱保护作用会释放出荧光素标记的寡核苷酸。

6-FAM phosphoramidite is an oligonucleotide label. There are several ways of labeling an oligonucleotide with fluorescein. The choice of label is diversified further, depending on the spectral requirements. 5′-fluorescein-CE phosphoramidite, derived from the single isomer 6-carboxyfluorescein, 5′-hexachlorofluorescein-CE phosphoramidite (HEX) and 5′-tetrachlorofluorescein-CE phosphoramidite (TET) can all be used to efficiently label an oligonucleotide at the 5′-end. Labeling the 3′-end of an oligo with fluorescein can be achieved using 3′-Fluorescein CPGs. Standard cleavage and deprotection with ammonium hydroxide liberates the fluorescein-labeled oligo when using any of these supports.
6-FAM phosphoramiditeis an oligonucleotide label