|英文名称||CruzFluor™ 488 succinimidyl ester|
Cruzfluor™488琥珀酰亚胺酯是FITC的高级蛋白质偶联荧光素衍生物，可替代AlexaFluor®488 NHS酯，Cy2®，DyLight®488和荧光素。Cruzfluor™488琥珀酰亚胺酯染料对蛋白质氨基具有选择性，各自的激发和发射最大值分别为491 nm和520 nm，并且偶联物更稳定，幅度更亮，并且没有FITC中发现的某些限制。CruzfluorTM 488不受pH 4-10的影响，与最大荧光所需的pH高于9相比有显着改善，并且不会因显微镜成像而遭受光漂白。此外，提供CruzFluor
Cruzfluor™ 488 succinimidyl ester is a superior protein conjugate fluorescein derivative for FITC and comparable replacement for Alexa Fluor® 488 NHS ester, Cy2®, DyLight® 488 and Fluorescein. Cruzfluor™ 488 succinimidyl ester dyes have selectivity for protein amino groups and a respective excitation and emission maxima of 491 nm and 520 nm, and the conjugates are more stable, magnitudes brighter and do not have some of the limitations that are found in FITC. The CruzfluorTM 488 is unaffected by pH 4-10, a marked improvement over a necessary pH above 9 for maximum fluorescence and does not suffer photobleaching for microscope imaging. Additionally, CruzFluor™ 488 (CFL 488) is offered conjugated to commonly used secondary antibodies for use in flow cytometry and immunofluorescence.|The CruzFluor|TM|family of products are a fluorescent labeling dye series that spans the full visible spectrum, and are useful to conjugate to nucleic acids, proteins and antibodies. The CruzFluor|TM|dyes are strong reagents for amine modification because they are incorporated with succinimidyl esters, which have a very strong ability to develop amide bonds that are as stable if not identical to the bonds found on a natural peptide.
CruzFluor™ 488 succinimidyl esteris a fluorophore dye used for labeling proteins, antibodies and nucleic acids
|溶解性||Soluble in water (Low), and DMSO.|