|英文名称||A3 2-AA Glycan|
|英文别名||A3 N-linked oligosaccharide, A3G3S3|
Sources A3 glyans are found on several mammalian glycoproteins including bovine serum fetuin (bSF). This product is typically purified from the oligosaccharide pool released from bSF by hydrazinolysis using a combination of HPLC and glycosidase digestion.
Form Dry. Dried by centrifugal evaporation from an aqueous solution containing approximately 50 mmol dm-3 of ammonium acetate (added to minimize acid-catalysed desialylation).
Molecular Weight 3000
Typically, 100 fmol to 10 pmol of the A3 2-AA glycan can be detected, dependent on the sensitivity of the fluorometer, HPLC or mass spectrometer.
Fluorescence detection: 360 nm excitation wavelength, 425 nm emission wavelength.
Labeled glycan mixtures may be separated and analysed by a variety of HPLC (high pressure liquid chromatography) methods using HPLC columns.
Columns are an especially powerful tool for the purification and analysis of labeled oligosaccharides from complex glycan mixtures. Please contact us for advise regarding your particular application.