A1F Procainamide Glycan
|产品名称||A1F Procainamide Glycan|
|英文名称||A1F Procainamide Glycan|
|英文别名||A1F N-linked oligosaccharide. α(2,6)/FA2G2S(6)1, G2FS1, FA2G2S1, F(6)A2G2S1, F(6)A2G(4)2S(6)1|
Procainamide labeled glycans can be analyzed by UHPLC, HPLC, ESI-MS, and LC-ESI-MS methods. Because of its improved ionization efficiency compared to 2-AB labeling it can permit identification of minor glycans (<1% relative peak area) by ESI-MS.
Sources: A1F glycan is found on many mammalian glycoproteins including IgG, gamma globulins, and many serum glycoproteins. This product is typically purified from the oligosaccharide pool released from porcine thyroglobulin by hydrazinolysis using a combination of HPLC and glycosidase digestion.
Form: Dry. Dried by centrifugal evaporation from an aqueous solution. Contains ammonium salt to stabilise against desialylation.
Molecular Weight: 2298
Mass free dye: 235.33
Mass Added to Glycan: 219.172
Fluorescence: λex = 310 nm, λem = 370 nm.
Amount: 20 pmols
HPLC Analysis of the A1F Procainamide Glycan
Fluorescence response Procainamide glycans show a much higher sensitivity as compared to 2–AB labeled glycans, providing peak heights three times higher than traditional 2-AB labeled glycans. The procainamide labeled sugars show comparable separation to HILIC-UHPLC assays with 2-AB.
Mass Spectrometry and Electrophoresis
Mass spectrometry response Due to its high ionization efficiency, procainamide shows much higher sensitivities the seen with 2-AB. When analyzed using ESI-MS and ESI-MS/MS, procainamide produces twenty-two times higher peaks than 2-AB. This provides a great platform for the detection and identification on minor peaks by MS/MS analysis.