FA2G1 Procainamide Glycan
|产品名称||FA2G1 Procainamide Glycan|
|英文名称||FA2G1 Procainamide Glycan|
|英文别名||FA2G1 N-linked oligosaccharide, G1F, F(6)A2G, NA2G1F|
Procainamide labeled glycans can be analyzed by UHPLC, HPLC, ESI-MS, and LC-ESI-MS methods. Because of its improved ionization efficiency compared to 2-AB labeling it can permit identification of minor glycans (<1% relative peak area) by ESI-MS.
Sources: FA2G1 glycan is found on several mammalian glycoproteins including asialo serum transferrin and fibrin. This product is typically purified from the oligosaccharide pool released from bovine serum by hydrazinolysis using a combination of HPLC and glycosidase digestion.
Form: Dry. Dried by centrifugal evaporation from an aqueous solution. Contains ammonium salt to stabilise against desialylation.
Molecular Weight: 1843
Mass free dye: 235.33
Mass Added to Glycan: 219.172
Fluorescence: λex = 310 nm, λem = 370 nm.
Amount: 20 pmols
HPLC Analysis of the FA2G1 Procainamide Glycan
Fluorescence response Procainamide glycans show a much higher sensitivity as compared to 2-AB labeled glycans, providing peak heights three times higher than traditional 2-AB labeled glycans. The procainamide labeled sugars show comparable separation to HILIC-UHPLC assays with 2-AB.
Mass Spectrometry and Electrophoresis
Mass spectrometry response Due to its high ionization efficiency, procainamide shows much higher sensitivities the seen with 2-AB. When analyzed using ESI-MS and ESI-MS/MS, procainamide produces twenty-two times higher peaks than 2-AB. This provides a great platform for the detection and identification on minor peaks by MS/MS analysis.