MAN-9 Procainamide Glycan
|产品名称||MAN-9 Procainamide Glycan|
|英文名称||MAN-9 Procainamide Glycan|
|英文别名||Man-9 N-linked oligosaccharide, Oligomannose 9 glycan|
procainamide labeled glycans can be analyzed by UHPLC, HPLC, ESI-MS, and LC-ESI-MS methods. Because of its improved ionization efficiency compared to 2-AB labeling it can permit identification of minor glycans (<1% relative peak area) by ESI-MS.
Sources: Man-9 glycan is found in a wide range of both plant and animal glycoproteins. This product is typically purified from the oligosaccharide pool released from porcine thyroglobulin by hydrazinolysis using a combination of HPLC and glycosidase digestion.
Form: Dry. Dried by centrifugal evaporation from an aqueous solution.
Molecular Weight: 2223
λex = 310 nm, λem = 370 nm.
: 20 pmols
HPLC Analysis of the Man-9 Procainamide Glycan
Procainamide glycans show a much higher sensitivity as compared to 2-AB labeled glycans, providing peak heights three times higher than traditional 2-AB labeled glycans. The procainamide labeled sugars show comparable separation to HILIC-UHPLC assays with 2-AB.
Mass Spectrometry and Electrophoresis
Due to its high ionization efficiency, procainamide shows much higher sensitivities the seen with 2-AB. When analyzed using ESI-MS and ESI-MS/MS, procainamide produces twenty-two times higher peaks than 2-AB. This provides a great platform for the detection and identification on minor peaks by MS/MS analysis.