β-半乳糖苷酶染色试剂盒
货 号：9860S
产品规格：1 Kit
原 产 地：CST
参考价格：2710 （参考价格，以实际价格为准）
优惠价格：
β-半乳糖苷酶染色试剂盒 9860S 1 Kit (125 35mm wells)
Product Includes Quantity (with Count)
10X Fixative Solution 15 ml
10X Staining Solution 15 ml
100X Solution A 1.5 ml
100X Solution B 1.5 ml
X-Gal 150 mg
The Senescence β-Galactosidase Staining Kit is designed to detect β-galactosidase activity at pH 6, a known characteristic of senescent cells not found in presenescent, quiescent or immortal cells (3). The kit includes all reagents necessary for this assay.
The kit detects β-galactosidase activity at pH 6 in cultured cells. β-galactosidase activity at pH 6 is present only in senescent cells and is not found in presenescent, quiescent or immortal cells.
Limited capacity to replicate is a defining characteristic of most normal cells and culminates in senescence, an arrested state in which the cell remains viable (1). Senescent cells are not stimulated to divide by serum or passage in culture, and senescence invokes a specific cell cycle profile that differs from most damage-induced arrest processes or contact inhibition (2). An enlarged cell size, expression of pH-dependent beta-galactosidase activity (3), and an altered pattern of gene expression (4,5) further characterize senescent cells.
1. Goldstein, S. (1990) Science 249, 1129-1133.
2. Sherwood, S. W. et al. (1988) Proc. Natl. Acad. Sci. , USA 85, 9086-9090.
3. Dimri, G. et al. (1995) Proc. Natl. Acad. Sci., USA 92, 9363-9367.
4. Cristofalo, V. J. et al. (1998) Crit. Rev. Eukaryot Gene Expr. 8, 43-80.
5. Linskens, M. H. et al. (1995) Nucleic Acid Res. 23, 3244-3251.
Product Specific References
Su, D. et al. (2009) J Biol Chem 284, 12153-64.
Swarbrick, A. et al. (2008) Proc Natl Acad Sci U S A 105, 5402-7.
Efeyan, A. et al. (2007) Cancer Res 67, 7350-7.
Baker, D.J. et al. (2004) Nat Genet 36, 744-9.
Boucher, M.J. et al. (2004) Am J Physiol Gastrointest Liver Physiol 286, G736-46.
Efeyan, A. et al. (2009) PLoS One 4, e5475.
Chang, C.J. et al. (2011) Nat Cell Biol 13, 317-23.
Delmore, J.E. et al. (2011) Cell 146, 904-17.
Blasco, R.B. et al. (2011) Cancer Cell 19, 652-63.
产品详细信息
β-半乳糖苷酶染色试剂盒 9860S 1 Kit (125 35mm wells)
Product Includes Quantity (with Count)
10X Fixative Solution 15 ml
10X Staining Solution 15 ml
100X Solution A 1.5 ml
100X Solution B 1.5 ml
X-Gal 150 mg
The Senescence β-Galactosidase Staining Kit is designed to detect β-galactosidase activity at pH 6, a known characteristic of senescent cells not found in presenescent, quiescent or immortal cells (3). The kit includes all reagents necessary for this assay.
The kit detects β-galactosidase activity at pH 6 in cultured cells. β-galactosidase activity at pH 6 is present only in senescent cells and is not found in presenescent, quiescent or immortal cells.
Limited capacity to replicate is a defining characteristic of most normal cells and culminates in senescence, an arrested state in which the cell remains viable (1). Senescent cells are not stimulated to divide by serum or passage in culture, and senescence invokes a specific cell cycle profile that differs from most damage-induced arrest processes or contact inhibition (2). An enlarged cell size, expression of pH-dependent beta-galactosidase activity (3), and an altered pattern of gene expression (4,5) further characterize senescent cells.
1. Goldstein, S. (1990) Science 249, 1129-1133.
2. Sherwood, S. W. et al. (1988) Proc. Natl. Acad. Sci. , USA 85, 9086-9090.
3. Dimri, G. et al. (1995) Proc. Natl. Acad. Sci., USA 92, 9363-9367.
4. Cristofalo, V. J. et al. (1998) Crit. Rev. Eukaryot Gene Expr. 8, 43-80.
5. Linskens, M. H. et al. (1995) Nucleic Acid Res. 23, 3244-3251.
Product Specific References
Su, D. et al. (2009) J Biol Chem 284, 12153-64.
Swarbrick, A. et al. (2008) Proc Natl Acad Sci U S A 105, 5402-7.
Efeyan, A. et al. (2007) Cancer Res 67, 7350-7.
Baker, D.J. et al. (2004) Nat Genet 36, 744-9.
Boucher, M.J. et al. (2004) Am J Physiol Gastrointest Liver Physiol 286, G736-46.
Efeyan, A. et al. (2009) PLoS One 4, e5475.
Chang, C.J. et al. (2011) Nat Cell Biol 13, 317-23.
Delmore, J.E. et al. (2011) Cell 146, 904-17.
Blasco, R.B. et al. (2011) Cancer Cell 19, 652-63.