香豆素
竞争性结合HDAC的荧光探针
有货
基本信息
| 产品名称 | 香豆素 |
|---|---|
| 英文名称 | Coumarin-SAHA |
| 英文别名 | Coumarin Suberoylanilide Hydroxamic Acid; N1-Hydroxy-N8-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)octanediamide |
| 运输条件 | 超低温冰袋运输 |
一般描述
香豆素-SAHA是一种SAHA衍生物，其中苯胺“帽”基团被7-氨基-4-甲基香豆素取代，从而产生竞争性结合HDAC的荧光探针。游离c-SAHA的荧光激发和发射最大值分别为325和400 nm，并与HDAC8结合时被50％淬灭。该探针可用于确定HDAC酶-抑制剂复合物的结合亲和力和解离解离速率，非常适合高通量筛选。
Coumarin-SAHA is a SAHA derivative where the anilino “cap” group is replaced by 7-amino-4-methylcoumarin to produce a fluorescent probe that competitively binds HDAC. The fluorescence excitation and emission maxima of free c-SAHA is 325 and 400 nm, respectively and is quenched by 50% when bound to HDAC8. This probe can be used to determine binding affinities and dissociation off-rates of HDAC enzyme-inhibitor complexes and is well-suited for high-throughput screening.
相关属性
| CAS编号 | 1260635-77-5 |
|---|---|
| 折光率 | n20D1.59 (Predicted) |
| 溶解性 | Soluble in DMSO (~5 mg/ml), DMF (~5 mg/ml), and 1:9 solution of DMF:PBS (pH 7.2) (~0.1 mg/ml). |
| 储存温度 | -20°C储存 |
| 密度 | ~1.3 g/cm3(Predicted) |
| 分子量 | 346.38 |
| 分子式 | C18H22N2O5 |
| 品牌 | Jinpan |
| Smiles | CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)CCCCCCC(=O)NO |
| PubChem CID | 53393956 |
| x_emission | λem 400 nm |
| x_excitation | λex 325 nm |