GATA4 Polyclonal Antibody for Western Blot, IF, ICC, IHC (P) and Flow
GATA4 Antibody (PA1-102) in IF
Immunofluorescent analysis of GATA4 (green) in embryoid body endoderm generated from Gibco ® Human Episomal iPSC Line grown on Geltrex® in Essential 8TM Medium. After 2 weeks in culture, EB were dissociated with TrypLETM and re-plated onto Geltrex®-coated multi-well plates. Cells were fixed, permeabilized and blocked for immunostaining. Cells were stained with a GATA4 polyclonal antibody (Product # PA1-102) at a dilution of 1:100 in 3% BSA/PBS blocking buffer overnight at 4°C, and then incubated with Alexa Fluor® 488 donkey anti rabbit antibody (Product # A21206) at a 1:500 dilution in conjunction with NucBlue® Fixed Cell Ready Probes® Reagent. After another 3 washes, images were taken on EVOS®Floid® Cell Imaging system at 10X magnification.