Rat IgG2a kappa Isotype Control, Alexa Fluor® 488 conjugate for IF, ICC, IHC, Flow and Ctrl
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Thrombospondin 1 Antibody (MA5-13398) in IF
Immunofluorescent analysis of Thrombospondin (green) showing staining in the secretion of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Thrombospondin monoclonal antibody (Product # MA5-13398) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
