胰岛素,Insulin, Human Recombinant 货号:91077C-250MG

胰岛素,Insulin, Human Recombinant 货号:91077C-250MG

胰岛素,Insulin, Human Recombinant

货  号:91077C-250MG

产品规格:250MG

原  产  地:sigma

参考价格:2703 (参考价格,以实际价格为准)

优惠价格:2433

产品详细信息

胰岛素,Insulin, Human Recombinant

产品描述

Biochem/physiol Actions

Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat.

Application

Serum-free medium supplements such as insulin are essential for long-term growth of commonly used mammalian cell lines. When insulin is absent from media, cell may exhibit disturbances in morphology and growth rate.
• Recombinant Human Insulin has been used in the media for adipogenic induction of mesenchymal stem cells.[1]
• It has been used as a supplement in the cell culture media of airway epithelial cells.[3]
• It has been used in the culture media for differentiation of cells, GLUT4 (glucose transporter type 4) translocation assay and to study interaction of insulin with insulin receptor via surface plasmon resonance.[2]

General description

Insulin, recombinant human (rHu) is identical in function and structure to the native human sequence. A hormone consisting of two polypeptide chains, rHu Insulin′s A-chain (21 amino acids) and B-chain (30 amino acids) are covalently linked by disulfide bonds between cysteine residues. This genetically engineered human insulin is a full sequence molecule producted by recombinant DNA technology in baker′s yeast.

产品详细信息

胰岛素,Insulin, Human Recombinant

产品描述

Biochem/physiol Actions

Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat.

Application

Serum-free medium supplements such as insulin are essential for long-term growth of commonly used mammalian cell lines. When insulin is absent from media, cell may exhibit disturbances in morphology and growth rate.
• Recombinant Human Insulin has been used in the media for adipogenic induction of mesenchymal stem cells.[1]
• It has been used as a supplement in the cell culture media of airway epithelial cells.[3]
• It has been used in the culture media for differentiation of cells, GLUT4 (glucose transporter type 4) translocation assay and to study interaction of insulin with insulin receptor via surface plasmon resonance.[2]

General description

Insulin, recombinant human (rHu) is identical in function and structure to the native human sequence. A hormone consisting of two polypeptide chains, rHu Insulin′s A-chain (21 amino acids) and B-chain (30 amino acids) are covalently linked by disulfide bonds between cysteine residues. This genetically engineered human insulin is a full sequence molecule producted by recombinant DNA technology in baker′s yeast.

Human CD19 (20-291) Protein, His Tag 货号:CD9-H52H2-50ug

Human CD19 (20-291) Protein, His Tag 货号:CD9-H52H2-50ug

Human CD19 (20-291) Protein, His Tag

货  号:CD9-H52H2-50ug

产品规格:50ug

原  产  地:

参考价格:3200 (参考价格,以实际价格为准)

优惠价格:2880

产品详细信息

 Human CD19 (20-291) Protein, His Tag

分子别名(Synonym)

CD19,B4,CVID3,MGC12802

表达区间及表达系统(Source)

Human CD19 (20-291), His Tag (CD9-H52H2) is expressed from human 293 cells (HEK293). It contains AA Pro 20 – Lys 291 (Accession # P15391-1).

Predicted N-terminus: Pro 20

Request for sequence

蛋白结构(Molecular Characterization)

Human CD19 (20-291) Protein, His Tag 货号:CD9-H52H2-50ug

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 32.0 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by reduced SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

电泳(SDS-PAGE)

Human CD19 (20-291) Protein, His Tag 货号:CD9-H52H2-50ug

Human CD19 (20-291), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

活性(Bioactivity)-ELISA

Human CD19 (20-291) Protein, His Tag 货号:CD9-H52H2-50ug

Immobilized FMC63 MAb at 2 μg/mL (100 μL/well) can bind Human CD19 (20-291), His Tag (Cat. No. CD9-H52H2) with a linear range of 10-78 ng/mL (QC tested).

Protocol

Human CD19 (20-291) Protein, His Tag 货号:CD9-H52H2-50ug

Immobilized Human CD19 (20-291), His Tag (Cat. No. CD9-H52H2) at 2 μg/mL (100 μL/well) on Nickel Coated Plate, can bind FMC63 (Mouse IgG2a) with a linear range of 2-13 ng/mL (Routinely tested).

Immobilized Human CD19 (20-291), His Tag (Cat. No. CD9-H52H2) at 0.1 μg/mL (100 μL/well) on MonRabTM Anti-His Tag (C-term) Antibody precoated (0.1 μg/well) plate, can bind FMC63 (Mouse IgG2a) with a linear range of 0.6-10 ng/mL (Routinely tested).

活性(Bioactivity)-SPR

Human CD19 (20-291) Protein, His Tag 货号:CD9-H52H2-50ug

FMC63 MAb (mouse lgG2a) captured on CM5 chip via anti-mouse antibodies surface, can bind Human CD19 (20-291), His Tag (Cat. No. CD9-H52H2) with an affinity constant of 2.95 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

CAR阳性表达率检测(Evaluation of CAR expression )

FACS Analysis of anti-CD19 CAR Expression

Human CD19 (20-291) Protein, His Tag 货号:CD9-H52H2-50ug

293 cells were transfected with FMC63-scFv and RFP tag. 2×105 of the cells were first incubated with A. His Tag-protein control. B. Recombinant human CD19 (20-291), His Tag (Cat. No. CD9-H52H2, 10 μg/ml). C. Recombinant human CD19, His Tag (Cat. No. CD9-H52H2, 10 μg/ml) and FMC63 (Mouse anti-CD19 antibody). The FITC Anti-6xHis tag antibody was used to analyse with FACS. RFP was used to evaluate CAR (FMC63-scFv) expression and FITC was used to evaluate the binding activity of recombinant human CD19 (20-291), His Tag (Cat. No. CD9-H52H2).

产品详细信息

 Human CD19 (20-291) Protein, His Tag

分子别名(Synonym)

CD19,B4,CVID3,MGC12802

表达区间及表达系统(Source)

Human CD19 (20-291), His Tag (CD9-H52H2) is expressed from human 293 cells (HEK293). It contains AA Pro 20 – Lys 291 (Accession # P15391-1).

Predicted N-terminus: Pro 20

Request for sequence

蛋白结构(Molecular Characterization)

Human CD19 (20-291) Protein, His Tag 货号:CD9-H52H2-50ug

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 32.0 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by reduced SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

电泳(SDS-PAGE)

Human CD19 (20-291) Protein, His Tag 货号:CD9-H52H2-50ug

Human CD19 (20-291), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

活性(Bioactivity)-ELISA

Human CD19 (20-291) Protein, His Tag 货号:CD9-H52H2-50ug

Immobilized FMC63 MAb at 2 μg/mL (100 μL/well) can bind Human CD19 (20-291), His Tag (Cat. No. CD9-H52H2) with a linear range of 10-78 ng/mL (QC tested).

Protocol

Human CD19 (20-291) Protein, His Tag 货号:CD9-H52H2-50ug

Immobilized Human CD19 (20-291), His Tag (Cat. No. CD9-H52H2) at 2 μg/mL (100 μL/well) on Nickel Coated Plate, can bind FMC63 (Mouse IgG2a) with a linear range of 2-13 ng/mL (Routinely tested).

Immobilized Human CD19 (20-291), His Tag (Cat. No. CD9-H52H2) at 0.1 μg/mL (100 μL/well) on MonRabTM Anti-His Tag (C-term) Antibody precoated (0.1 μg/well) plate, can bind FMC63 (Mouse IgG2a) with a linear range of 0.6-10 ng/mL (Routinely tested).

活性(Bioactivity)-SPR

Human CD19 (20-291) Protein, His Tag 货号:CD9-H52H2-50ug

FMC63 MAb (mouse lgG2a) captured on CM5 chip via anti-mouse antibodies surface, can bind Human CD19 (20-291), His Tag (Cat. No. CD9-H52H2) with an affinity constant of 2.95 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

CAR阳性表达率检测(Evaluation of CAR expression )

FACS Analysis of anti-CD19 CAR Expression

Human CD19 (20-291) Protein, His Tag 货号:CD9-H52H2-50ug

293 cells were transfected with FMC63-scFv and RFP tag. 2×105 of the cells were first incubated with A. His Tag-protein control. B. Recombinant human CD19 (20-291), His Tag (Cat. No. CD9-H52H2, 10 μg/ml). C. Recombinant human CD19, His Tag (Cat. No. CD9-H52H2, 10 μg/ml) and FMC63 (Mouse anti-CD19 antibody). The FITC Anti-6xHis tag antibody was used to analyse with FACS. RFP was used to evaluate CAR (FMC63-scFv) expression and FITC was used to evaluate the binding activity of recombinant human CD19 (20-291), His Tag (Cat. No. CD9-H52H2).

Recombinant Human IFN-β 货号:AF-300-02B

Recombinant Human IFN-β 货号:AF-300-02B

Recombinant Human IFN-β

货  号:AF-300-02B

产品规格:20ug

原  产  地:USA

参考价格:1140 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

Recombinant Human IFN-β

产品编号:

AF-300-02B
别名:
Leukocyte interferon, B cell interferon, Type I interferon
描述:
Proteins of this family play an important role in inducing non-specific resistance against a broad range of viral infections. They also affect cell proliferation and modulate immune responses. Produced by peripheral blood leukocytes and lymphoblastoid cells, IFNα is an acid stable molecule that signals through IFNα/βR, which is also used by IFNβ. Both IFNs have similar anti-viral activity and regulate expression of MHC class I antigens. IFNα contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity. Recombinant human IFNβ is an 20.0 kDa protein containing 166 amino acid residues. Manufactured using all non-animal reagents.
氨基酸序列:
MSYNLLGFLQ RSSNFQCQKL LWQLNGRLEY CLKDRMNFDI PEEIKQLQQF QKEDAALTIY EMLQNIFAIF RQDSSSTGWN ETIVENLLAN VYHQINHLKT VLEEKLEKED FTRGKLMSSL HLKRYYGRIL HYLKAKEYSH CAWTIVRVEI LRNFYFINRL TGYLRN
来源:
E.coli
纯度:
Greater than 95% by SDS-PAGE gel and HPLC analyses.
生物活性:
Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.25 ng/ml, corresponding to a specific activity of ≥ 4 x 106 units/mg.

产品详细信息

Recombinant Human IFN-β

产品编号:

AF-300-02B
别名:
Leukocyte interferon, B cell interferon, Type I interferon
描述:
Proteins of this family play an important role in inducing non-specific resistance against a broad range of viral infections. They also affect cell proliferation and modulate immune responses. Produced by peripheral blood leukocytes and lymphoblastoid cells, IFNα is an acid stable molecule that signals through IFNα/βR, which is also used by IFNβ. Both IFNs have similar anti-viral activity and regulate expression of MHC class I antigens. IFNα contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity. Recombinant human IFNβ is an 20.0 kDa protein containing 166 amino acid residues. Manufactured using all non-animal reagents.
氨基酸序列:
MSYNLLGFLQ RSSNFQCQKL LWQLNGRLEY CLKDRMNFDI PEEIKQLQQF QKEDAALTIY EMLQNIFAIF RQDSSSTGWN ETIVENLLAN VYHQINHLKT VLEEKLEKED FTRGKLMSSL HLKRYYGRIL HYLKAKEYSH CAWTIVRVEI LRNFYFINRL TGYLRN
来源:
E.coli
纯度:
Greater than 95% by SDS-PAGE gel and HPLC analyses.
生物活性:
Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.25 ng/ml, corresponding to a specific activity of ≥ 4 x 106 units/mg.

Anti-Human CD230 (PrP) PE 货号:12-9230-42

Anti-Human CD230 (PrP) PE 货号:12-9230-42

Anti-Human CD230 (PrP) PE

货  号:12-9230-42

产品规格:100 tests

原  产  地:USA

参考价格:2988 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

Anti-Human CD230 (PrP) PE   12-9230-42

   
Anti-Human CD230 (PrP) PE
Also known as: Prion protein
Clone: 4D5
RUO: For Research Use Only. Not for use in diagnostic procedures.

Host     Mouse
Isotype     IgG1, kappa
Reactivity     Human
Conjugate     PE
Laser     Blue Laser, Green Laser, Yellow-Green Laser
Excite     488 – 561 nm
Reported Applications     Flow Cytometric Analysis

References: Durig J, Giese A, Schulz-Schaeffer W, Rosenthal C, Schmucker U, Bieschke J, Duhrsen U, Kretzschmar HA. Differential constitutive and activation-dependent expression of prion protein in human peripheral blood leucocytes. Br J Haematol. 2000 Mar;108(3):488-95. (PubMed)

Barclay GR, Hope J, Birkett CR, Turner ML. Distribution of cell-associated prion protein in normal adult blood determined by flow cytometry. Br J Haematol. 1999 Dec;107(4):804-14. (PubMed)

Holada K, Simak J, Risitano AM, Maciejewski J, Young NS, Vostal JG. Activated platelets of patients with paroxysmal nocturnal hemoglobinuria express cellular prion protein. Blood. 2002 Jul 1;100(1):341-3. (PubMed)

产品详细信息

Anti-Human CD230 (PrP) PE   12-9230-42

   
Anti-Human CD230 (PrP) PE
Also known as: Prion protein
Clone: 4D5
RUO: For Research Use Only. Not for use in diagnostic procedures.

Host     Mouse
Isotype     IgG1, kappa
Reactivity     Human
Conjugate     PE
Laser     Blue Laser, Green Laser, Yellow-Green Laser
Excite     488 – 561 nm
Reported Applications     Flow Cytometric Analysis

References: Durig J, Giese A, Schulz-Schaeffer W, Rosenthal C, Schmucker U, Bieschke J, Duhrsen U, Kretzschmar HA. Differential constitutive and activation-dependent expression of prion protein in human peripheral blood leucocytes. Br J Haematol. 2000 Mar;108(3):488-95. (PubMed)

Barclay GR, Hope J, Birkett CR, Turner ML. Distribution of cell-associated prion protein in normal adult blood determined by flow cytometry. Br J Haematol. 1999 Dec;107(4):804-14. (PubMed)

Holada K, Simak J, Risitano AM, Maciejewski J, Young NS, Vostal JG. Activated platelets of patients with paroxysmal nocturnal hemoglobinuria express cellular prion protein. Blood. 2002 Jul 1;100(1):341-3. (PubMed)

Anti-Human CD267 (TACI) PE 货号:12-9217-41

Anti-Human CD267 (TACI) PE 货号:12-9217-41

Anti-Human CD267 (TACI) PE

货  号:12-9217-41

产品规格:25 tests

原  产  地:USA

参考价格:1788 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

Anti-Human CD267 (TACI) PE  12-9217-41

Also known as: TNFRSF13B
Clone: 11H3
RUO: For Research Use Only. Not for use in diagnostic procedures.
Host     Mouse
Isotype     IgG2a
Reactivity     Human
Conjugate     PE
Laser     Blue Laser, Green Laser, Yellow-Green Laser
Emit     575 nm
Excite     488 – 561 nm
Reported Applications     Flow Cytometric Analysis

References: von Bulow GU, van Deursen JM, Bram RJ. Regulation of the T-independent humoral response by TACI.Immunity. 2001 May;14(5):573-82.

Ng LG, Sutherland AP, Newton R, Qian F, Cachero TG, Scott ML, Thompson JS, Wheway J, Chtanova T, Groom J, Sutton IJ, Xin C, Tangye SG, Kalled SL, Mackay F, Mackay CR. B cell-activating factor belonging to the TNF family (BAFF)-R is the principal BAFF receptor facilitating BAFF costimulation of circulating T and B cells.J Immunol. 2004 Jul 15;173(2):807-17.

Novak AJ, Darce JR, Arendt BK, Harder B, Henderson K, Kindsvogel W, Gross JA, Greipp PR, Jelinek DF. Expression of BCMA, TACI, and BAFF-R in multiple myeloma: a mechanism for growth and survival.Blood. 2004 Jan 15;103(2):689-94.

Sakurai D, Kanno Y, Hase H, Kojima H, Okumura K, Kobata T. TACI attenuates antibody production costimulated by BAFF-R and CD40. Eur J Immunol. 2007 Jan;37(1):110-8. PubMed

Sakurai D, Hase H, Kanno Y, Kojima H, Okumura K, Kobata T. TACI regulates IgA production by APRIL in collaboration with HSPG. Blood. 2007 Apr 1;109(7):2961-7. PubMed

Seshasayee D, Valdez P, Yan M, Dixit VM, Tumas D, Grewal IS. Loss of TACI causes fatal lymphoproliferation and autoimmunity, establishing TACI as an inhibitory BLyS receptor.Immunity. 2003 Feb;18(2):279-88.

Yan M, Wang H, Chan B, Roose-Girma M, Erickson S, Baker T, Tumas D, Grewal IS, Dixit VM. Activation and accumulation of B cells in TACI-deficient mice.Nat Immunol. 2001 Jul;2(7):638-43.

产品详细信息

Anti-Human CD267 (TACI) PE  12-9217-41

Also known as: TNFRSF13B
Clone: 11H3
RUO: For Research Use Only. Not for use in diagnostic procedures.
Host     Mouse
Isotype     IgG2a
Reactivity     Human
Conjugate     PE
Laser     Blue Laser, Green Laser, Yellow-Green Laser
Emit     575 nm
Excite     488 – 561 nm
Reported Applications     Flow Cytometric Analysis

References: von Bulow GU, van Deursen JM, Bram RJ. Regulation of the T-independent humoral response by TACI.Immunity. 2001 May;14(5):573-82.

Ng LG, Sutherland AP, Newton R, Qian F, Cachero TG, Scott ML, Thompson JS, Wheway J, Chtanova T, Groom J, Sutton IJ, Xin C, Tangye SG, Kalled SL, Mackay F, Mackay CR. B cell-activating factor belonging to the TNF family (BAFF)-R is the principal BAFF receptor facilitating BAFF costimulation of circulating T and B cells.J Immunol. 2004 Jul 15;173(2):807-17.

Novak AJ, Darce JR, Arendt BK, Harder B, Henderson K, Kindsvogel W, Gross JA, Greipp PR, Jelinek DF. Expression of BCMA, TACI, and BAFF-R in multiple myeloma: a mechanism for growth and survival.Blood. 2004 Jan 15;103(2):689-94.

Sakurai D, Kanno Y, Hase H, Kojima H, Okumura K, Kobata T. TACI attenuates antibody production costimulated by BAFF-R and CD40. Eur J Immunol. 2007 Jan;37(1):110-8. PubMed

Sakurai D, Hase H, Kanno Y, Kojima H, Okumura K, Kobata T. TACI regulates IgA production by APRIL in collaboration with HSPG. Blood. 2007 Apr 1;109(7):2961-7. PubMed

Seshasayee D, Valdez P, Yan M, Dixit VM, Tumas D, Grewal IS. Loss of TACI causes fatal lymphoproliferation and autoimmunity, establishing TACI as an inhibitory BLyS receptor.Immunity. 2003 Feb;18(2):279-88.

Yan M, Wang H, Chan B, Roose-Girma M, Erickson S, Baker T, Tumas D, Grewal IS, Dixit VM. Activation and accumulation of B cells in TACI-deficient mice.Nat Immunol. 2001 Jul;2(7):638-43.

Anti-Human CD200 PE 货号:12-9200-41

Anti-Human CD200 PE 货号:12-9200-41

Anti-Human CD200 PE

货  号:12-9200-41

产品规格:25 tests

原  产  地:USA

参考价格:1188 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

Anti-Human CD200 PE   12-9200-41

 Anti-Human CD200 PE
Also known as: OX-2
Clone: OX104
RUO: For Research Use Only. Not for use in diagnostic procedures.

Host     Mouse
Isotype     IgG1, kappa
Reactivity     Human
Conjugate     PE
Laser     Blue Laser, Green Laser, Yellow-Green Laser
Excite     488 – 561 nm
Reported Applications     Flow Cytometric Analysis

References: Wright GJ, Cherwinski H, Foster-Cuevas M, Brooke G, Puklavec MJ, Bigler M, Song Y, Jenmalm M, Gorman D, McClanahan T, Liu MR, Brown MH, Sedgwick JD, Phillips JH, Barclay AN. Characterization of the CD200 receptor family in mice and humans and their interactions with CD200. J Immunol. 2003 Sep 15;171(6):3034-46.

Barclay AN, Wright GJ, Brooke G, Brown MH. CD200 and membrane protein interactions in the control of myeloid cells. Trends Immunol. 2002 Jun;23(6):285-90. Review.

Wright GJ, Jones M, Puklavec MJ, Brown MH, Barclay AN. The unusual distribution of the neuronal/lymphoid cell surface CD200 (OX2) glycoprotein is conserved in humans. Immunology. 2001 Feb;102(2):173-9. (OX104, FC IHf, PubMed)

12-9200-41 Anti-Human CD200 PE  25 tests eBioscience 1,188.00
12-9200-42 Anti-Human CD200 PE  100 tests eBioscience 2,868.00
12-9201-42 Anti-Human CD200 Receptor PE  100 tests eBioscience 2,988.00

产品详细信息

Anti-Human CD200 PE   12-9200-41

 Anti-Human CD200 PE
Also known as: OX-2
Clone: OX104
RUO: For Research Use Only. Not for use in diagnostic procedures.

Host     Mouse
Isotype     IgG1, kappa
Reactivity     Human
Conjugate     PE
Laser     Blue Laser, Green Laser, Yellow-Green Laser
Excite     488 – 561 nm
Reported Applications     Flow Cytometric Analysis

References: Wright GJ, Cherwinski H, Foster-Cuevas M, Brooke G, Puklavec MJ, Bigler M, Song Y, Jenmalm M, Gorman D, McClanahan T, Liu MR, Brown MH, Sedgwick JD, Phillips JH, Barclay AN. Characterization of the CD200 receptor family in mice and humans and their interactions with CD200. J Immunol. 2003 Sep 15;171(6):3034-46.

Barclay AN, Wright GJ, Brooke G, Brown MH. CD200 and membrane protein interactions in the control of myeloid cells. Trends Immunol. 2002 Jun;23(6):285-90. Review.

Wright GJ, Jones M, Puklavec MJ, Brown MH, Barclay AN. The unusual distribution of the neuronal/lymphoid cell surface CD200 (OX2) glycoprotein is conserved in humans. Immunology. 2001 Feb;102(2):173-9. (OX104, FC IHf, PubMed)

12-9200-41 Anti-Human CD200 PE  25 tests eBioscience 1,188.00
12-9200-42 Anti-Human CD200 PE  100 tests eBioscience 2,868.00
12-9201-42 Anti-Human CD200 Receptor PE  100 tests eBioscience 2,988.00

Anti-Human CD185 (CXCR5) PE 货号:12-9185-41

Anti-Human CD185 (CXCR5) PE 货号:12-9185-41

Anti-Human CD185 (CXCR5) PE

货  号:12-9185-41

产品规格:25 tests

原  产  地:USA

参考价格:1548 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

Anti-Human CD185 (CXCR5) PE   12-9185-41

Also known as: C-X-C chemokine receptor type 5, BLR1, MDR15
Clone: MU5UBEE
RUO: For Research Use Only. Not for use in diagnostic procedures.
Host     Mouse
Isotype     IgG2b, kappa
Reactivity     Human, Rhesus
Conjugate     PE
Laser     Blue Laser, Green Laser, Yellow-Green Laser
Excite     488 – 561 nm
Reported Applications     Flow Cytometric Analysis

References: Reinhardt RL, Liang HE, Locksley RM. Cytokine-secreting follicular T cells shape the antibody repertoire. Nat Immunol. 2009 Apr;10(4):385-93.

Muller G, Hopken UE, Stein H, Lipp M. Systemic immunoregulatory and pathogenic functions of homeostatic chemokine receptors. J Leukoc Biol. 2002 Jul; 72(1):1-8.

Förster R, Mattis AE, Kremmer E, Wolf E, Brem G, Lipp M. A putative chemokine receptor, BLR1, directs B cell migration to defined lymphoid organs and specific anatomic compartments of the spleen. Cell. 1996 Dec 13;87(6):1037-47.

Barella L, Loetscher M, Tobler A, Baggiolini M, Moser B. Sequence variation of a novel heptahelical leucocyte receptor through alternative transcript formation. Biochem J. 1995 Aug 1;309 ( Pt 3):773-9.

Dobner T, Wolf I, Emrich T, Lipp M. Differentiation-specific expression of a novel G protein-coupled receptor from Burkitt’s lymphoma. Eur J Immunol. 1992 Nov;22(11):2795-9.

12-9185-41 Anti-Human CD185 (CXCR5) PE  25 tests eBioscience 1,548.00
12-9185-42 Anti-Human CD185 (CXCR5) PE  100 tests eBioscience 3,948.00

产品详细信息

Anti-Human CD185 (CXCR5) PE   12-9185-41

Also known as: C-X-C chemokine receptor type 5, BLR1, MDR15
Clone: MU5UBEE
RUO: For Research Use Only. Not for use in diagnostic procedures.
Host     Mouse
Isotype     IgG2b, kappa
Reactivity     Human, Rhesus
Conjugate     PE
Laser     Blue Laser, Green Laser, Yellow-Green Laser
Excite     488 – 561 nm
Reported Applications     Flow Cytometric Analysis

References: Reinhardt RL, Liang HE, Locksley RM. Cytokine-secreting follicular T cells shape the antibody repertoire. Nat Immunol. 2009 Apr;10(4):385-93.

Muller G, Hopken UE, Stein H, Lipp M. Systemic immunoregulatory and pathogenic functions of homeostatic chemokine receptors. J Leukoc Biol. 2002 Jul; 72(1):1-8.

Förster R, Mattis AE, Kremmer E, Wolf E, Brem G, Lipp M. A putative chemokine receptor, BLR1, directs B cell migration to defined lymphoid organs and specific anatomic compartments of the spleen. Cell. 1996 Dec 13;87(6):1037-47.

Barella L, Loetscher M, Tobler A, Baggiolini M, Moser B. Sequence variation of a novel heptahelical leucocyte receptor through alternative transcript formation. Biochem J. 1995 Aug 1;309 ( Pt 3):773-9.

Dobner T, Wolf I, Emrich T, Lipp M. Differentiation-specific expression of a novel G protein-coupled receptor from Burkitt’s lymphoma. Eur J Immunol. 1992 Nov;22(11):2795-9.

12-9185-41 Anti-Human CD185 (CXCR5) PE  25 tests eBioscience 1,548.00
12-9185-42 Anti-Human CD185 (CXCR5) PE  100 tests eBioscience 3,948.00

Anti-Human Clec6 PE 货号:12-9113-41

Anti-Human Clec6 PE 货号:12-9113-41

Anti-Human Clec6 PE

货  号:12-9113-41

产品规格:25 tests

原  产  地:USA

参考价格:1428 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

Anti-Human Clec6 PE  12-9113-41

Also known as: CLEC4D, CLECSF8
Clone: 9B9
RUO: For Research Use Only. Not for use in diagnostic procedures.
Host     Mouse
Isotype     IgG2b, kappa
Reactivity     Human
Conjugate     PE
Laser     Blue Laser, Green Laser, Yellow-Green Laser
Excite     488 – 561 nm
Reported Applications     Flow Cytometric Analysis

References: Kerscher B, Willment JA, Brown GD. The Dectin-2 family of C-type lectin-like receptors: an update. Int Immunol. 2013 May;25(5):271-7.

Graham LM, Gupta V, Schafer G, Reid DM, Kimberg M, Dennehy KM, Hornsell WG, Guler R, Campanero-Rhodes MA, Palma AS, Feizi T, Kim SK, Sobieszczuk P, Willment JA, Brown GD. The C-type Lectin Receptor CLECSF8 (CLEC4D) Is Expressed by Myeloid Cells and Triggers Cellular Activation through Syk Kinase. J Biol Chem. 2012 Jul 27;287(31):25964-74.

Arce I, Martínez-Muñoz L, Roda-Navarro P, Fernández-Ruiz E. The human C-type lectin CLECSF8 is a novel monocyte/macrophage endocytic receptor. Eur J Immunol. 2004 Jan;34(1):210-20.

12-9113-41 Anti-Human Clec6 PE  25 tests eBioscience 1,428.00
12-9113-42 Anti-Human Clec6 PE  100 tests eBioscience 3,588.00

产品详细信息

Anti-Human Clec6 PE  12-9113-41

Also known as: CLEC4D, CLECSF8
Clone: 9B9
RUO: For Research Use Only. Not for use in diagnostic procedures.
Host     Mouse
Isotype     IgG2b, kappa
Reactivity     Human
Conjugate     PE
Laser     Blue Laser, Green Laser, Yellow-Green Laser
Excite     488 – 561 nm
Reported Applications     Flow Cytometric Analysis

References: Kerscher B, Willment JA, Brown GD. The Dectin-2 family of C-type lectin-like receptors: an update. Int Immunol. 2013 May;25(5):271-7.

Graham LM, Gupta V, Schafer G, Reid DM, Kimberg M, Dennehy KM, Hornsell WG, Guler R, Campanero-Rhodes MA, Palma AS, Feizi T, Kim SK, Sobieszczuk P, Willment JA, Brown GD. The C-type Lectin Receptor CLECSF8 (CLEC4D) Is Expressed by Myeloid Cells and Triggers Cellular Activation through Syk Kinase. J Biol Chem. 2012 Jul 27;287(31):25964-74.

Arce I, Martínez-Muñoz L, Roda-Navarro P, Fernández-Ruiz E. The human C-type lectin CLECSF8 is a novel monocyte/macrophage endocytic receptor. Eur J Immunol. 2004 Jan;34(1):210-20.

12-9113-41 Anti-Human Clec6 PE  25 tests eBioscience 1,428.00
12-9113-42 Anti-Human Clec6 PE  100 tests eBioscience 3,588.00

Anti-Human CD289 (TLR9) PE 货号:12-9099-80

Anti-Human CD289 (TLR9) PE 货号:12-9099-80

Anti-Human CD289 (TLR9) PE

货  号:12-9099-80

产品规格:25 ug

原  产  地:USA

参考价格:1788 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

Anti-Human CD289 (TLR9) PE   12-9099-80

Also known as: TLR-9, toll-like receptor 9
Clone: eB72-1665
RUO: For Research Use Only. Not for use in diagnostic procedures.
Host     Rat
Isotype     IgG2a, kappa
Reactivity     Human
Conjugate     PE
Laser     Blue Laser, Green Laser, Yellow-Green Laser
Excite     488 – 561 nm
Reported Applications     Intracellular Staining Followed by Flow Cytometric Analysis

References: Peduzzi E, Groeper C, et al. 2007. Local activation of the innate immune system in Buruli ulcer lesions. J Invest Dermatol. 127(3):638-45. (eB72-1665, IHC paraffin, PubMed)

Jozsef L, Khreiss T, El Kebir D, Filep JG. Activation of TLR-9 induces IL-8 secretion through peroxynitrite signaling in human neutrophils. J Immunol. 2006 Jan 15;176(2):1195-202. (eB72-1665, FC, PubMed)

Schroeder JT, Bieneman AP, et al. 2005. TLR9- and FcepsilonRI-mediated responses oppose one another in plasmacytoid dendritic cells by down-regulating receptor expression. J Immunol. 175(9):5724-31. (eB72-1665, WB, PubMed)

Dasari P, Nicholson IC, et al. 2005. Expression of toll-like receptors on B lymphocytes. Cell Immunol. 236(1-2):140-5. (eB72-1665, IC flow, PubMed)

Latz E, Schoenemeyer A, Visintin A, Fitzgerald KA, Monks BG, Knetter CF, Lien E, Nilsen NJ, Espevik T, Golenbock DT. 2004. TLR9 signals after translocating from the ER to CpG DNA in the lysosome. Nat Immunol. 5(2):190-8.

Juliane Platz, Christoph Beisswenger, Alexander Dalpke, Rembert Koczulla, Olaf Pinkenburg, Claus Vogelmeier, and Robert Bals. 2004. Microbial DNA Induces a Host Defense Reaction of Human Respiratory Epithelial Cells. J. Immunol. 173: 1219 – 1223.

Leifer CA, Kennedy MN, et al. 2004. TLR9 is localized in the endoplasmic reticulum prior to stimulation. J Immunol. 173(2):1179-83. (Immunofluorescence and IP, PubMed)

12-9099-80 Anti-Human CD289 (TLR9) PE  25 ug eBioscience 1,788.00
12-9099-82 Anti-Human CD289 (TLR9) PE  100 ug eBioscience 4,188.00

产品详细信息

Anti-Human CD289 (TLR9) PE   12-9099-80

Also known as: TLR-9, toll-like receptor 9
Clone: eB72-1665
RUO: For Research Use Only. Not for use in diagnostic procedures.
Host     Rat
Isotype     IgG2a, kappa
Reactivity     Human
Conjugate     PE
Laser     Blue Laser, Green Laser, Yellow-Green Laser
Excite     488 – 561 nm
Reported Applications     Intracellular Staining Followed by Flow Cytometric Analysis

References: Peduzzi E, Groeper C, et al. 2007. Local activation of the innate immune system in Buruli ulcer lesions. J Invest Dermatol. 127(3):638-45. (eB72-1665, IHC paraffin, PubMed)

Jozsef L, Khreiss T, El Kebir D, Filep JG. Activation of TLR-9 induces IL-8 secretion through peroxynitrite signaling in human neutrophils. J Immunol. 2006 Jan 15;176(2):1195-202. (eB72-1665, FC, PubMed)

Schroeder JT, Bieneman AP, et al. 2005. TLR9- and FcepsilonRI-mediated responses oppose one another in plasmacytoid dendritic cells by down-regulating receptor expression. J Immunol. 175(9):5724-31. (eB72-1665, WB, PubMed)

Dasari P, Nicholson IC, et al. 2005. Expression of toll-like receptors on B lymphocytes. Cell Immunol. 236(1-2):140-5. (eB72-1665, IC flow, PubMed)

Latz E, Schoenemeyer A, Visintin A, Fitzgerald KA, Monks BG, Knetter CF, Lien E, Nilsen NJ, Espevik T, Golenbock DT. 2004. TLR9 signals after translocating from the ER to CpG DNA in the lysosome. Nat Immunol. 5(2):190-8.

Juliane Platz, Christoph Beisswenger, Alexander Dalpke, Rembert Koczulla, Olaf Pinkenburg, Claus Vogelmeier, and Robert Bals. 2004. Microbial DNA Induces a Host Defense Reaction of Human Respiratory Epithelial Cells. J. Immunol. 173: 1219 – 1223.

Leifer CA, Kennedy MN, et al. 2004. TLR9 is localized in the endoplasmic reticulum prior to stimulation. J Immunol. 173(2):1179-83. (Immunofluorescence and IP, PubMed)

12-9099-80 Anti-Human CD289 (TLR9) PE  25 ug eBioscience 1,788.00
12-9099-82 Anti-Human CD289 (TLR9) PE  100 ug eBioscience 4,188.00

Anti-Human/Mouse phospho-STAT4 (Y693) PE 货号:12-9044-41

Anti-Human/Mouse phospho-STAT4 (Y693) PE 货号:12-9044-41

Anti-Human/Mouse phospho-STAT4 (Y693) PE

货  号:12-9044-41

产品规格:25 tests

原  产  地:USA

参考价格:1548 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

Anti-Human/Mouse phospho-STAT4 (Y693) PE   12-9044-41

Also known as: Signal transducer and activator of transcription 4
Clone: 4LURPIE
RUO: For Research Use Only. Not for use in diagnostic procedures.
Host     Mouse
Isotype     IgG1, kappa
Reactivity     Human, Mouse
Conjugate     PE
Laser     Blue Laser, Green Laser, Yellow-Green Laser
Emit     578 nm
Excite     488 – 561 nm
Reported Applications     Intracellular Staining Followed by Flow Cytometric Analysis

References: Saraiva M, Christensen JR, Veldhoen M, Murphy TL, Murphy KM, O’Garra A. Interleukin-10 production by Th1 cells requires interleukin-12-induced STAT4 transcription factor and ERK MAP kinase activation by high antigen dose. Immunity. 2009 Aug 21;31(2):209-19.

Ramos HJ, Davis AM, George TC, Farrar JD. IFN-alpha is not sufficient to drive Th1 development due to lack of stable T-bet expression. J Immunol. 2007 Sep 15;179(6):3792-803.

Berenson LS, Gavrieli M, Farrar JD, Murphy TL, Murphy KM. Distinct characteristics of murine STAT4 activation in response to IL-12 and IFN-alpha. J Immunol. 2006 Oct 15;177(8):5195-203.

Harrington LE, Hatton RD, Mangan PR, Turner H, Murphy TL, Murphy KM, Weaver CT. Interleukin 17-producing CD4+ effector T cells develop via a lineage distinct from the T helper type 1 and 2 lineages. Nat Immunol. 2005 Nov;6(11):1123-32.

12-9044-41 Anti-Human/Mouse phospho-STAT4 (Y693) PE  25 tests eBioscience 1,548.00
12-9044-42 Anti-Human/Mouse phospho-STAT4 (Y693) PE  100 tests eBioscience 3,588.00

产品详细信息

Anti-Human/Mouse phospho-STAT4 (Y693) PE   12-9044-41

Also known as: Signal transducer and activator of transcription 4
Clone: 4LURPIE
RUO: For Research Use Only. Not for use in diagnostic procedures.
Host     Mouse
Isotype     IgG1, kappa
Reactivity     Human, Mouse
Conjugate     PE
Laser     Blue Laser, Green Laser, Yellow-Green Laser
Emit     578 nm
Excite     488 – 561 nm
Reported Applications     Intracellular Staining Followed by Flow Cytometric Analysis

References: Saraiva M, Christensen JR, Veldhoen M, Murphy TL, Murphy KM, O’Garra A. Interleukin-10 production by Th1 cells requires interleukin-12-induced STAT4 transcription factor and ERK MAP kinase activation by high antigen dose. Immunity. 2009 Aug 21;31(2):209-19.

Ramos HJ, Davis AM, George TC, Farrar JD. IFN-alpha is not sufficient to drive Th1 development due to lack of stable T-bet expression. J Immunol. 2007 Sep 15;179(6):3792-803.

Berenson LS, Gavrieli M, Farrar JD, Murphy TL, Murphy KM. Distinct characteristics of murine STAT4 activation in response to IL-12 and IFN-alpha. J Immunol. 2006 Oct 15;177(8):5195-203.

Harrington LE, Hatton RD, Mangan PR, Turner H, Murphy TL, Murphy KM, Weaver CT. Interleukin 17-producing CD4+ effector T cells develop via a lineage distinct from the T helper type 1 and 2 lineages. Nat Immunol. 2005 Nov;6(11):1123-32.

12-9044-41 Anti-Human/Mouse phospho-STAT4 (Y693) PE  25 tests eBioscience 1,548.00
12-9044-42 Anti-Human/Mouse phospho-STAT4 (Y693) PE  100 tests eBioscience 3,588.00