产品介绍
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组成
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50次×50μL反应体系
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200次×50μL反应体系
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2×SYBR Green qPCR Mix
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1.25 mL
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1.25 mL×4
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产品参数
Ex(nm)：497
Em(nm)：525
储存：-20 ℃ 避光保存6个月，使用前充分溶解混匀。短期使用可放在4 ℃，避免反复冻融。
制品说明
本制品是采用SYBR Green I嵌合荧光法进行Real Time PCR的专用试剂。已经将DNA聚合酶、dNTP、特殊稳定剂、优化的反应缓冲液、BSA和SYBR Green I等试剂预混成一种适合Real Time PCR反应检测用2×Premix Type试剂，具有灵敏度高、特异性强、稳定性好等特点。使用时只需加入模板、引物和水，便可在宽广的定量区域内得到良好的标准曲线，对目的基因进行准确定量检测，重复性好，可信度高。
注意事项
1.本制品不含参比染料ROX，客户可根据qPCR仪器技术指导决定是否需要加ROX参比染料，用于消除信号本底以及校正孔与孔之间产生的荧光信号误差。
2.本制品含SYBR Green I 强光下易分解，降低灵敏度，使用时避免长时间强光照射本制品。
3.建议在冰上配制PCR反应液，再放入PCR仪器中扩增。可以提高扩增特异性，减少背景。
4.本制品含有4 mM MgCl2（反应体系终浓度是2 mM Mg2+），可用25mM MgCl2 优化Mg2+浓度。
建议PCR条件（以50 μL 反应体系为例，反应液配制请在冰上进行）
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Components
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Volume
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Final Concentration
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2×SYBR Green qPCR Mix
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25μL
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1×
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DNA Template
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2μL
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as required
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Forward Primer (10μM)
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1μL
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0.2 μM each
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Reverse Primer (10μM)
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1μL
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0.2 μM each
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ddH2O to final volume
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50μL
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Not applicable
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PCR 循环
94℃ 2～3 min
94℃ 10～20 sec
55～65℃ 10～20 sec 35～45 cycles
72℃ 20～60 sec
72℃ 5～10 min
Product Introduction
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Composition
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50T×50μL reaction system
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200T×50μL reaction system
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|
2×SYBR Green qPCR Mix
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1.25 mL
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1.25 mL×4
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Product parameter
Ex(nm)：497
Em(nm)：525
Storage: Store at -20 ℃ in the dark for 6 months. Dissolve and mix thoroughly before use. It can be stored at 4℃ for short-term use to avoid repeated freezing and thawing.
Product description
This product is a special reagent for Real Time PCR using SYBR Green I chimeric fluorescence method. Reagents such as DNA polymerase, dNTP, special stabilizers, optimized reaction buffer, BSA and SYBR Green I have been premixed into a 2×Premix Type reagent suitable for Real Time PCR reaction detection. It has high sensitivity, strong specificity, Features such as good stability. When using, only need to add template, primer and water, a good standard curve can be obtained in a wide quantitative area, and the target gene can be accurately and quantitatively detected, with good repeatability and high reliability.
Precautions
1. This product does not contain the reference dye ROX. Customers can decide whether to add ROX reference dye according to the qPCR instrument technical guidance to eliminate the signal background and correct the fluorescence signal error generated between the wells.
2. This product contains SYBR Green I. It is easy to decompose under strong light and reduce sensitivity. Avoid long-term strong light exposure to this product.
3. It is recommended to prepare the PCR reaction solution on ice and put it into the PCR instrument for amplification. It can improve amplification specificity and reduce background.
4. This product contains 4 mM MgCl2 (the final concentration of the reaction system is 2 mM Mg2+), and 25mM MgCl2 can be used to optimize the Mg2+ concentration.
Suggested PCR conditions (take a 50 μL reaction system as an example, please prepare the reaction solution on ice)
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Components
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Volume
|
Final Concentration
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2×SYBR Green qPCR Mix
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25μL
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1×
|
|
DNA Template
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2μL
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as required
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|
Forward Primer (10μM)
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1μL
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0.2 μM each
|
|
Reverse Primer (10μM)
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1μL
|
0.2 μM each
|
|
ddH2O to final volume
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50μL
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Not applicable
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PCR cycle
94℃ 2～3 min
94℃ 10～20 sec
55～65℃ 10～20 sec 35～45 cycles
72℃ 20～60 sec
72℃ 5～10 min
