异硫氰酸荧光素-葡聚糖

一般描述

葡聚糖是一种无水葡萄糖聚合物。由大约 95% 的α-D-(166)键组成。其余的 a (163) 键构成了葡聚糖的分支。分支长度相互矛盾的数据表明平均分支长度小于 3 个葡萄糖单位。但是，其他方法研究表明存在大于 50 个葡萄糖单位的分支。已发现天然葡聚糖的分子量 (MW) 范围为 900 万至 5 亿。较低分子量的葡聚糖具有略少的支化 ，并具有更窄的分子量分布范围。分子量大于10,000 的葡聚糖似乎是高度分支化的。随着分子量的增加，葡聚糖分子的对称性增强。具有 2,000

应用

FITC-葡聚糖被广泛用于利用微荧光法对微循环和细胞渗透性的研究。 FITC-葡聚糖已用于研究植物细胞壁的孔隙率和毛细管渗透性。血浆蛋白已被显示不可与FITC-葡聚糖进行结合。 用异硫氰酸荧光素标记的葡聚糖可用于动物的灌注研究。 FITC-葡聚糖已用于心血管、微循环、灌注、细胞单层和细胞膜通透性研究，作为可支持血流、膜损伤、血管引流和肾脏消除等过程进行测量的荧光通量示踪剂化合物。小的 FITC-葡聚糖已用于对内吞作用和细胞连接渗透性等过程进行测量。70kDa 的 FITC-葡聚糖 （FD-70）可用作开发将分子递送到原生质体中的模型化合物，并研究从水凝胶、脂质体等结构中释放药物的方法。它可用于微血管和细胞单层渗透性研究。

制备说明

通常在水中以 25mg/ml 或更高的浓度对 FITC 葡聚糖的溶解进行检测。溶液应避光保存。FITC-葡聚糖在体内可稳定超过 24 小时。

General Description

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three glucose units. However, other methods indicate branches of greater than 50 glucose units exist. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.

Application

FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. FITC-dextran has been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to FITC-dextran. FITC-dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. Small FITC-dextrans are also used to measure processes such as endocytosis and cell junction permeability. FITC-dextran 70kDa (FD-70) may be used as a model compound for development of methods to deliver molecules into protoplasts and to study drug release from structures such as hydrogels and liposomes. It may be used in microvascular and cell monolayer permeability studies. Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.

异硫氰酸荧光素-葡聚糖

一般描述

右旋糖酐是一种无水葡萄糖聚合物。由约 95% 的 α-D-(166) 键组成。其余 (163) 连接可解释右旋糖酐的分支。分支长度的冲突数据表明平均分支长度小于 3 个葡萄糖单位。然而，其他方法表明存在超过 50 个葡萄糖单位的分支。已发现天然右旋糖酐分子量 (MW) 在 900 万 ～5 亿范围内。较低分子量的右旋糖酐将表现出略少的分支，并且分子量分布范围更窄。分子量大于 10,000 的右旋糖酐表现得好像它们是高度支链。随着分子量的增加，葡聚糖分子的对称性增强。分子量为 2,00

应用

FITC-右旋糖酐利用显微荧光法广泛用于微循环和细胞渗透性研究。FITC-右旋糖酐已被用于研究植物细胞壁多孔性和毛细管渗透性。已证明血浆蛋白不与 FITC-右旋糖酐结合。 FITC-右旋糖酐作为荧光通量示踪化合物用于心血管、微循环、灌注、细胞单层和细胞膜渗透性研究，支持血流、膜损伤、血管引流和肾脏消除等过程的测量。小的 FITC-右旋糖酐也用于测量细胞内吞和细胞连接通透性等过程。荧光素-葡聚糖，20 kDa，可用于研究细胞过程，如内吞和渗透压。FITC-右旋糖酐-20 可用于研究内皮细胞或上皮细胞单层孔隙度、细胞层内顶端基底侧运动和细胞连接渗透性。FITC-右旋糖酐-20 可作为研究氢原子和胶原微棒等结构药物释放的模型化合物。 用异硫氰酸荧光素标记的葡聚糖可用于动物的灌注研究。

制备说明

通常检测浓度等于或高于 25 mg/mL 的 FITC 葡聚糖在水中的溶解度。溶液应避光保存。在体内，FITC-右旋糖酐可稳定 24 小时以上。

General Description

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three glucose units. However, other methods indicate branches of greater than 50 glucose units exist. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.

Application

FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. FITC-dextran has been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to FITC-dextran. FITC-dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. Small FITC-dextrans are also used to measure processes such as endocytosis and cell junction permeability. Fluorescein-dextran, 20 kDa, may be used to study cell processes such as endocytosis and osmoporation. FITC-dextran-20 may be used to study endothelial or epithelial monolayer porosity, apical-basolateral movements within cell layers and cell junction permeability. FITC-dextran-20 may be used as a model compound to study drug release from structures such as hydrogens and collagen minirods. Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.

异硫氰酸荧光素-葡聚糖

一般描述

右旋糖酐是一种无水葡萄糖聚合物。由约 95% 的 α-D-(166) 键组成。其余 (163) 连接可解释右旋糖酐的分支。分支长度的冲突数据表明平均分支长度小于 3 个葡萄糖单位。然而，其他研究表明存在超过 50 个葡萄糖单位的分支。已发现天然右旋糖酐分子量 (MW) 在 900 万 ～5 亿范围内。较低分子量的右旋糖酐将表现出略少的分支，并且分子量分布范围更窄。分子量大于 10,000 的右旋糖酐表现出高度支链状。随着分子量的增加，葡聚糖分子的对称性增强。分子量为2,000至10,

应用

FITC-右旋糖酐作为荧光通量示踪化合物用于心血管、微循环、灌注、细胞单层和细胞膜渗透性研究，支持血流、膜损伤、血管引流和肾脏消除等过程的测量。小FITC-右旋糖酐也用于测量内吞作用和细胞连接通透性等过程。500 kDa 荧光素-右旋糖酐可用作脑脊液 (CSF) 示踪剂。FITC-右旋糖酐-500 可用于研究内皮细胞或上皮细胞单层孔隙度、细胞层内顶端基底侧运动和细胞连接渗透性。 FITC-右旋糖酐广泛用于利用微荧光法的微循环和细胞渗透性研究。FITC-右旋糖酐已被用于研究植物细胞壁孔隙率和毛细管渗透性。已证实血浆蛋白不与 FITC-右旋糖酐结合。 用异硫氰酸荧光素标记的葡聚糖可用于动物的灌注研究。

制备说明

通常以25mg / ml或更高的浓度测试FITC右旋糖酐在水中的溶解度。溶液应避光保存。在体内，FITC-右旋糖酐可稳定 24 小时以上。

General Description

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three glucose units. However, other methods indicate branches of greater than 50 glucose units exist. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.

Application

FITC-dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. Small FITC-dextrans are also used to measure processes such as endocytosis and cell junction permeability. Fluorescein-dextran, 500 kDa, may be used as a cerebral spinal fluid (CSF) tracer. FITC-dextran-500 may be used to study endothelial or epithelial monolayer porosity, apical-basolateral movements within cell layers and cell junction permeability. FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. FITC-dextran has been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to FITC-dextran. Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.

异硫氰酸荧光素-葡聚糖

应用

FITC-葡聚糖在心血管、微循环、灌注、细胞单层和细胞膜通透性研究中用作荧光通量示踪化合物，可实现对血流、膜损伤、血管引流和肾脏清除等过程进行检测。 小FITC-葡聚糖也可用于检测内吞作用和细胞连接通透性等过程。FITC-葡聚糖70 kDa（FD-70）可用作模型化合物，用于开发将分子递送到原生质体中和研究从水凝胶和脂质体等结构释放药物的方法。FD-70可用于微血管和细胞单层的通透性研究。 用异硫氰酸荧光素标记的葡聚糖可用于动物的灌注研究。

Application

FITC-dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. Small FITC-dextrans are also used to measure processes such as endocytosis and cell junction permeability. FITC-dextran 70 kDa (FD-70) may be used as a model compound for development of methods to deliver molecules into protoplasts and to study drug release from structures such as hydrogels and liposomes. FD-70 may be used in microvascular and cell monolayer permeability studies. Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.

异硫氰酸荧光素-葡聚糖

应用

用异硫氰酸荧光素标记的葡聚糖可用于动物的灌注研究。

其他说明

已发现天然右旋糖酐的分子量 (MW) 在 900 万至 500 万范围内。较低分子量的右旋糖酐将表现出略微较少的支化，并具有更窄的分子量分布范围。分子量大于 10,000 的葡聚糖似乎是表现为高度支化的。 随着分子量的增加，葡聚糖分子的对称性增强。 分子量为2,000至10,000的葡聚糖分子表现出可膨胀线圈的特性。在分子量低于 2,000 时，右旋糖酐更像棒状。右旋糖酐的分子量用以下一种或多种方法测定：低角度激光散射、尺寸排阻色谱法、铜络合、和蒽酮试剂、显色还原末端糖测定和粘度。

制备说明

通常以25mg/ml或更高的浓度对FITC葡聚糖在水中的溶解度进行测试。 溶液应保证避光。 在体内，FITC-葡聚糖可保持稳定超过24小时。

Application

Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.

Other Notes

Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.

异硫氰酸荧光素 – 葡聚糖 500000-偶联物

应用

荧光素异硫氰酸酯-葡聚糖500000-偶联物被用作荧光组织渗透性测定试剂，以及用作评估水凝胶和脂质囊泡等生物材料的扩散探针。

Description

Fluorescein isothiocyanate – Dextran 500000-Conjugate is used as a fluorescent tissue permeability assay reagent and as a diffusion probe for evaluation of biomaterials such as hydrogels and lipid vesicles.

异硫氰酸荧光素-葡聚糖

一般描述

葡聚糖是葡萄糖酐聚合物。 由大约95%的α-D-(166)键组成。 剩余的(163)键是葡聚糖的分支。分支长度的冲突数据意味着平均分支长度小于三个葡萄糖单位。但是，其他方法表明存在大于 50 个葡萄糖单位的分支。已发现的天然葡聚糖分子量（MW）在900万至5亿范围内。MW较低的葡聚糖分支略少，MW分布范围较窄。MW大于10,000 的葡聚糖表现得似乎它们是高度支化的。 随着MW的增加，葡聚糖分子的对称性增强。 MW为2,000至10,000的葡聚糖表现出可膨胀线圈的特性。

应用

FITC-葡聚糖在心血管、微循环、灌注、细胞单层和细胞膜通透性研究中用作荧光通量示踪化合物，可实现对血流、膜损伤、血管引流和肾脏清除等过程进行检测。小FITC-葡聚糖也可用于检测内吞作用等过程。荧光素-葡聚糖，40 kDa，可用于研究细胞内吞活性。FITC-葡聚糖-40可用于研究内皮单层孔隙度和细胞层内的顶端-基底外侧运动。 FITC-葡聚糖被广泛用于运用显微荧光法进行微循环和细胞通透性研究。 FITC-葡聚糖被用于植物细胞壁孔隙度和毛细血管通透性研究。研究表明，血浆蛋白不与FITC-葡聚糖结合。 用异硫氰酸荧光素标记的葡聚糖可用于动物的灌注研究。

制备说明

通常以25 mg/ml或更高的浓度测试FITC葡聚糖在水中的溶解度。 溶液应避光保存。 在体内，FITC-葡聚糖稳定24小时以上。

General Description

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three glucose units. However, other methods indicate branches of greater than 50 glucose units exist. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.

Application

FITC-dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. Small FITC-dextrans are also used to measure processes such as endocytosis. Fluorescein-dextran, 40 kDa, may be used for the study of cell endocytic activity. FITC-dextran-40 may be used to study endothelial monolayer porosity and apical-basolateral movements within cell layers. FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. FITC-dextran has been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to FITC-dextran. Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.

异硫氰酸荧光素-葡聚糖

应用

用异硫氰酸荧光素标记的葡聚糖可用于动物的灌注研究。

Application

Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.

异硫氰酸荧光素-葡聚糖

一般描述

葡聚糖是一种脱水葡萄糖的聚合物。它由大约95％的α-D-(166)键组成。剩余的(163)连接依靠葡聚糖的支链。关于支链长度的矛盾数据意味着平均的支链长度小于三个葡萄糖单位。然而，其他方法表明存在大于50个葡萄糖单元的支链。已发现天然葡聚糖的分子量（MW）在900万至5亿的范围内。较低分子量的葡聚糖将表现出略微更少的支链化并具有更窄的分子量分布范围。分子量大于10,000的葡聚糖会表现的像是高度支链化的一样。随着分子量的增加，葡聚糖分子会获得更好的对称性。分子量为2,000至10,0

应用

FITC-葡聚糖可广泛用于利用微荧光法的微循环和细胞渗透性研究。FITC-葡聚糖已被用于研究植物细胞壁的孔隙率以及毛细管的渗透性。血浆蛋白已证明不会与FITC-葡聚糖结合。 FITC-葡聚糖作为荧光通量示踪剂化合物，可被用于心血管、微循环、灌注、细胞单层和细胞膜通透性研究，支持血流、膜损伤、血管引流和肾脏消除等过程的测量。 小的FITC-葡聚糖也可用于测量内吞作用和细胞连接通透性等过程。荧光素-葡聚糖，150kDa可助力于血脑屏障（BBB）的完整性评估以及进行荧光微淋巴造影（FML）。 荧光素-葡聚糖150可用作微循环血管通量示踪剂。 用异硫氰酸荧光素标记的葡聚糖可用于动物的灌注研究。

制备说明

通常以25 mg/ml或更高的浓度测试FITC葡聚糖在水中的溶解度。溶液应避光保存。在体内，FITC-葡聚糖可稳定超过24小时。

General Description

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three glucose units. However, other methods indicate branches of greater than 50 glucose units exist. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.

Application

FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. FITC-dextran has been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to FITC-dextran. FITC-dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. Small FITC-dextrans are also used to measure processes such as endocytosis and cell junction permeability. Fluorescein-dextran, 150 kDa may be used to help assess the integrity of the blood brain barrier (BBB) and to perform fluorescence microlymphography (FML). Fluorescein-dextran 150 is used as a microcirculation vascular flux tracer. Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.