Cas(150347-56-1), 5(6)-TAMRA, 5-(and-6)-Carboxytetramethylrhodamine,5(6)-TAMRA,

5(6)-TAMRA

97%

有货

Cas(150347-56-1), 5(6)-TAMRA, 5-(and-6)-Carboxytetramethylrhodamine,5(6)-TAMRA,

CAS编号 150347-56-1 | 品牌:Jinpan
5(6)-TAMRA

MSDS

质检证书(CoA)

相似产品

  • 分子式 C25H22N2O5
  • 分子量430.46

货号 (SKU) 包装规格 是否现货 价格 数量
C272939-100mg 100mg 期货 Cas(150347-56-1), 5(6)-TAMRA, 5-(and-6)-Carboxytetramethylrhodamine,5(6)-TAMRA,  
C272939-250mg 250mg 期货 Cas(150347-56-1), 5(6)-TAMRA, 5-(and-6)-Carboxytetramethylrhodamine,5(6)-TAMRA,  
C272939-1g 1g 期货 Cas(150347-56-1), 5(6)-TAMRA, 5-(and-6)-Carboxytetramethylrhodamine,5(6)-TAMRA,  

基本信息

产品名称 5(6)-TAMRA
英文名称 5(6)-TAMRA
别名 5-(and-6)-Carboxytetramethylrhodamine
英文别名 5-(and-6)-Carboxytetramethylrhodamine
规格或纯度 97%
运输条件 超低温冰袋运输

一般描述

Fluorescent Dye Carboxylic Acids and Their Succinimidyl Esters

Succinimidyl esters are proven to be the best reagents for amine modifications because the amide bonds that are formed are essentially identical to, and as stable as the natural peptide bonds. These reagents are generally stable and show good reactivity and selectivity with aliphatic amines.  There are few factors that need be considered when SE compounds are used for conjugation reaction: 

1). Solvents:For the most part, reactive dyes are hydrophobic molecules and should be dissolved in anhydrous dimethylformamide (DMF) or dimethylsulfoxide (DMSO). 

2). Reaction pH:The labeling reactions of amines with succinimidyl esters are strongly pH dependent. Amine-reactive reagents react with non-protonated aliphatic amine groups, including the terminal amines of proteins and the e-amino groups of lysines. Thus amine acylation reactions are usually carried out above pH 7.5. Protein modifications by succinimidyl esters can typically be done at pH 7.5-8.5, whereas isothiocyanates may require a pH 9.0-10.0 for optimal conjugations. 

3).Reaction Buffers:Buffers that contain free amines such as Tris and glycine and thiol compounds must be avoided when using an amine-reactive reagent. Ammonium salts (such as ammonium sulfate and ammonium acetate) that are widely used for protein precipitation must also be removed (such as viadinlysis) before performing dye conjugations. 

4). Reaction Temperature:Most conjugations are done at room temperature. However, either elevated or reduced temperature may be required for a particular labeling reaction.

Features and Biological Applications

TAMRA is one of the most popular fluorophores used in various bioconjugations. TMR is a bright organe fluorophore. 5(6)-TAMRA is the mixture of two carboxy tetramethylrhodamine (TMR) isomers.  It is used to modify amino and hydroxy groups using EDC-mediated couplings. It can be readily convert to the amine-reactive 5(6)-TAMRA, SE. 

Product parameter

Ex(nm)541

Em(nm)565

References

1.Evans NA, et al. (2001). Visualizing differences in ligand-induced beta-arrestin-GFP interactions and trafficking between three recently characterized G protein-coupled receptors. J Neurochem77, 476-85.

2.Hess KL, et al. (1997). A novel flow cytometric method for quantifying phagocytosis of apoptotic cells. Cytometry27, 145-52.

Fluorescent Dye Carboxylic Acids and Their Succinimidyl Esters

Succinimidyl esters are proven to be the best reagents for amine modifications because the amide bonds that are formed are essentially identical to, and as stable as the natural peptide bonds. These reagents are generally stable and show good reactivity and selectivity with aliphatic amines.  There are few factors that need be considered when SE compounds are used for conjugation reaction: 

1). Solvents:For the most part, reactive dyes are hydrophobic molecules and should be dissolved in anhydrous dimethylformamide (DMF) or dimethylsulfoxide (DMSO). 

2). Reaction pH:The labeling reactions of amines with succinimidyl esters are strongly pH dependent. Amine-reactive reagents react with non-protonated aliphatic amine groups, including the terminal amines of proteins and the e-amino groups of lysines. Thus amine acylation reactions are usually carried out above pH 7.5. Protein modifications by succinimidyl esters can typically be done at pH 7.5-8.5, whereas isothiocyanates may require a pH 9.0-10.0 for optimal conjugations. 

3).Reaction Buffers:Buffers that contain free amines such as Tris and glycine and thiol compounds must be avoided when using an amine-reactive reagent. Ammonium salts (such as ammonium sulfate and ammonium acetate) that are widely used for protein precipitation must also be removed (such as viadinlysis) before performing dye conjugations. 

4). Reaction Temperature:Most conjugations are done at room temperature. However, either elevated or reduced temperature may be required for a particular labeling reaction.

Features and Biological Applications

TAMRA is one of the most popular fluorophores used in various bioconjugations. TMR is a bright organe fluorophore. 5(6)-TAMRA is the mixture of two carboxy tetramethylrhodamine (TMR) isomers.  It is used to modify amino and hydroxy groups using EDC-mediated couplings. It can be readily convert to the amine-reactive 5(6)-TAMRA, SE. 

Product parameter

Ex(nm)541

Em(nm)565

References

1.Evans NA, et al. (2001). Visualizing differences in ligand-induced beta-arrestin-GFP interactions and trafficking between three recently characterized G protein-coupled receptors. J Neurochem77, 476-85.

2.Hess KL, et al. (1997). A novel flow cytometric method for quantifying phagocytosis of apoptotic cells. Cytometry27, 145-52.

相关属性

CAS编号 150347-56-1
储存温度 避光,-20°C储存,干燥
分子量 430.46
分子式 C25H22N2O5
品牌 Jinpan
关联CAS 98181-63-6

Cas(91809-66-4), 5-TAMRA, 5-Carboxytetramethylrhodamine,5-TAMRA,

5-羧基四甲基罗丹明

>97%

有货

Cas(91809-66-4), 5-TAMRA, 5-Carboxytetramethylrhodamine,5-TAMRA,

CAS编号 91809-66-4 | 品牌:Jinpan
5-TAMRA

MSDS

质检证书(CoA)

相似产品

  • 分子式 C25H22N2O5
  • 分子量430.45
  • MDL号 MFCD01073638
  • PubChem编号 2762602

货号 (SKU) 包装规格 是否现货 价格 数量
C272928-50mg 50mg 现货 Cas(91809-66-4), 5-TAMRA, 5-Carboxytetramethylrhodamine,5-TAMRA,  
C272928-100mg 100mg 现货 Cas(91809-66-4), 5-TAMRA, 5-Carboxytetramethylrhodamine,5-TAMRA,  
C272928-250mg 250mg 现货 Cas(91809-66-4), 5-TAMRA, 5-Carboxytetramethylrhodamine,5-TAMRA,  
C272928-1g 1g 现货 Cas(91809-66-4), 5-TAMRA, 5-Carboxytetramethylrhodamine,5-TAMRA,  
C272928-5g 5g 现货 Cas(91809-66-4), 5-TAMRA, 5-Carboxytetramethylrhodamine,5-TAMRA,  

基本信息

产品名称 5-羧基四甲基罗丹明
英文名称 5-TAMRA
英文别名 5-Carboxytetramethylrhodamine;5-TAMRA
规格或纯度 >97%
运输条件 超低温冰袋运输

一般描述

Fluorescent Dye Carboxylic Acids and Their Succinimidyl Esters

Succinimidyl esters are proven to be the best reagents for amine modifications because the amide bonds that are formed are essentially identical to, and as stable as the natural peptide bonds. These reagents are generally stable and show good reactivity and selectivity with aliphatic amines.  There are few factors that need be considered when SE compounds are used for conjugation reaction: 

1). Solvents:For the most part, reactive dyes are hydrophobic molecules and should be dissolved in anhydrous dimethylformamide (DMF) or dimethylsulfoxide (DMSO). 

2). Reaction pH:The labeling reactions of amines with succinimidyl esters are strongly pH dependent. Amine-reactive reagents react with non-protonated aliphatic amine groups, including the terminal amines of proteins and the e-amino groups of lysines. Thus amine acylation reactions are usually carried out above pH 7.5. Protein modifications by succinimidyl esters can typically be done at pH 7.5-8.5, whereas isothiocyanates may require a pH 9.0-10.0 for optimal conjugations. 

3).Reaction Buffers:Buffers that contain free amines such as Tris and glycine and thiol compounds must be avoided when using an amine-reactive reagent. Ammonium salts (such as ammonium sulfate and ammonium acetate) that are widely used for protein precipitation must also be removed (such as viadinlysis) before performing dye conjugations. 

4). Reaction Temperature:Most conjugations are done at room temperature. However, either elevated or reduced temperature may be required for a particular labeling reaction.

Features and Biological Applications

5-TAMRA is the purified single isomer of 5(6)-TAMRA. It is widely used to label peptides and proteins. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-TAMRA and 6-TAMRA might affect some biological properties of the underlying conjugates.

Product parameter

Ex(nm)541

Em(nm)568

References

1. Evans NA, et al. (2001). Visualizing differences in ligand-induced beta-arrestin-GFP interactions and trafficking between three recently characterized G protein-coupled receptors. J Neurochem77, 476-85.

2. Hahn, M., et al., Influence of fluorophor dye labels on the migration behavior of polymerase chain reaction—amplified short tandem repeats during denaturing capillary electrophoresis.Electrophoresis2001, 22, 2691-700.

3. Yoo H and Juliano RL (2000). Enhanced delivery of antisense oligonucleotides with fluorophore- conjugated PAMAM dendrimers. Nucleic Acids Res28, 4225-31. 

Fluorescent Dye Carboxylic Acids and Their Succinimidyl Esters

Succinimidyl esters are proven to be the best reagents for amine modifications because the amide bonds that are formed are essentially identical to, and as stable as the natural peptide bonds. These reagents are generally stable and show good reactivity and selectivity with aliphatic amines.  There are few factors that need be considered when SE compounds are used for conjugation reaction: 

1). Solvents:For the most part, reactive dyes are hydrophobic molecules and should be dissolved in anhydrous dimethylformamide (DMF) or dimethylsulfoxide (DMSO). 

2). Reaction pH:The labeling reactions of amines with succinimidyl esters are strongly pH dependent. Amine-reactive reagents react with non-protonated aliphatic amine groups, including the terminal amines of proteins and the e-amino groups of lysines. Thus amine acylation reactions are usually carried out above pH 7.5. Protein modifications by succinimidyl esters can typically be done at pH 7.5-8.5, whereas isothiocyanates may require a pH 9.0-10.0 for optimal conjugations. 

3).Reaction Buffers:Buffers that contain free amines such as Tris and glycine and thiol compounds must be avoided when using an amine-reactive reagent. Ammonium salts (such as ammonium sulfate and ammonium acetate) that are widely used for protein precipitation must also be removed (such as viadinlysis) before performing dye conjugations. 

4). Reaction Temperature:Most conjugations are done at room temperature. However, either elevated or reduced temperature may be required for a particular labeling reaction.

Features and Biological Applications

5-TAMRA is the purified single isomer of 5(6)-TAMRA. It is widely used to label peptides and proteins. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-TAMRA and 6-TAMRA might affect some biological properties of the underlying conjugates.

Product parameter

Ex(nm)541

Em(nm)568

References

1. Evans NA, et al. (2001). Visualizing differences in ligand-induced beta-arrestin-GFP interactions and trafficking between three recently characterized G protein-coupled receptors. J Neurochem77, 476-85.

2. Hahn, M., et al., Influence of fluorophor dye labels on the migration behavior of polymerase chain reaction—amplified short tandem repeats during denaturing capillary electrophoresis.Electrophoresis2001, 22, 2691-700.

3. Yoo H and Juliano RL (2000). Enhanced delivery of antisense oligonucleotides with fluorophore- conjugated PAMAM dendrimers. Nucleic Acids Res28, 4225-31. 

相关属性

CAS编号 91809-66-4
敏感性 对光和湿度敏感
储存温度 避光,-20°C储存,干燥
MDL号 MFCD01073638
分子量 430.45
分子式 C25H22N2O5
品牌 Jinpan
Smiles CN(C)c1ccc2c(c1)[o+]c1cc(ccc1c2c1ccc(cc1C(=O)[O-])C(=O)O)N(C)C
PubChem CID 2762602