Annexin V-FITC (Reagent) 1 mL 4700-100 1144
标签归档：fitc
Annexin V-FITC (Reagent) 1 mL 4700-100
Annexin V-FITC (Reagent) 1 mL 4700-100 1144
Annexin V-FITC (Reagent) 1 mL 4700-100
Annexin V-FITC (Reagent) 1 mL 4700-100 1144
蛋白L,FITC-Labeled Recombinant Protein L 货号：RPL-PF141
蛋白L,FITC-Labeled Recombinant Protein L
货 号：RPL-PF141
产品规格：200ug
原 产 地：
参考价格：1200 （参考价格，以实际价格为准）
优惠价格：
产品详细信息
FITC-Labeled Recombinant Protein L
蛋白L,FITC-Labeled Recombinant Protein L
分子别名（Synonym）
RPL,Protein L
表达区间及表达系统（Source）
FITC-Labeled Recombinant Protein L (Cat. No. RPL-PF141) is expressed from human HEK293 cells. It is the FITC labeled form of Recombinant Protein L (Cat. No. RPL-P3141).
Request for sequence
蛋白结构（Molecular Characterization）
FITC-Labeled Recombinant Protein L is fused with a polyhistidine tag at the N-terminus. The reducing (R) protein migrates as 40-45 kDa in SDS-PAGE.
Conjugate
FITC
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
标记（Labeling）
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.
蛋白标记度（FITC:Protein Ratio)
The FITC to protein molar ratio is 5-7.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
活性（Bioactivity）-ELISA
Immobilized Yervoy Ipilumimab at 10 μg/mL (100 μL/well) can bind FITC-Labeled Recombinant Protein L (Cat. No. RPL-PF141) with a linear range of 20-156 ng/mL (Ex.488 nm/Em.535 nm) (QC tested).
产品详细信息
FITC-Labeled Recombinant Protein L
蛋白L,FITC-Labeled Recombinant Protein L
分子别名（Synonym）
RPL,Protein L
表达区间及表达系统（Source）
FITC-Labeled Recombinant Protein L (Cat. No. RPL-PF141) is expressed from human HEK293 cells. It is the FITC labeled form of Recombinant Protein L (Cat. No. RPL-P3141).
Request for sequence
蛋白结构（Molecular Characterization）
FITC-Labeled Recombinant Protein L is fused with a polyhistidine tag at the N-terminus. The reducing (R) protein migrates as 40-45 kDa in SDS-PAGE.
Conjugate
FITC
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
标记（Labeling）
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.
蛋白标记度（FITC:Protein Ratio)
The FITC to protein molar ratio is 5-7.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
活性（Bioactivity）-ELISA
Immobilized Yervoy Ipilumimab at 10 μg/mL (100 μL/well) can bind FITC-Labeled Recombinant Protein L (Cat. No. RPL-PF141) with a linear range of 20-156 ng/mL (Ex.488 nm/Em.535 nm) (QC tested).
FITC标记细胞凋亡检测试剂盒 货号：KT40003
FITC标记细胞凋亡检测试剂盒
货 号：KT40003
产品规格：100T
原 产 地：abgent
参考价格：1600 （参考价格，以实际价格为准）
优惠价格：
产品详细信息
FITC标记细胞凋亡检测试剂盒 KT40003 100T
FITC Annexin V Apoptosis Detection Kit
Catalog #KT40003
25tests¥ 670.00
100tests¥ 1,600.00
产品详细信息
FITC标记细胞凋亡检测试剂盒 KT40003 100T
FITC Annexin V Apoptosis Detection Kit
Catalog #KT40003
25tests¥ 670.00
100tests¥ 1,600.00
FITC anti-mouse CD45 货号：103108
FITC anti-mouse CD45
货 号：103108
产品规格：500ug
原 产 地：biolegend
参考价格：2470 （参考价格，以实际价格为准）
优惠价格：
产品详细信息
FITC anti-mouse CD45 103108 500ug
Clone: 30-F11 (See other available formats)
Isotype: Rat IgG2b, κ
Isotype Control: FITC Rat IgG2b, κ Isotype Ctrl
Reactivity: Mouse
Immunogen: Mouse thymus or spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:
FC – Quality tested
Application
References:
1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
8. Liu F, et al. 2012. Blood. 119:3295. PubMed
9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
10. Ito K, et al. 2014. PNAS. 111:3080. PubMed
11. Abdala-Valencia H, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:482. PubMed
12. Zhang K, et al. 2014. PLoS Pathog. 10:1004385. PubMed
产品详细信息
FITC anti-mouse CD45 103108 500ug
Clone: 30-F11 (See other available formats)
Isotype: Rat IgG2b, κ
Isotype Control: FITC Rat IgG2b, κ Isotype Ctrl
Reactivity: Mouse
Immunogen: Mouse thymus or spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:
FC – Quality tested
Application
References:
1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
8. Liu F, et al. 2012. Blood. 119:3295. PubMed
9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
10. Ito K, et al. 2014. PNAS. 111:3080. PubMed
11. Abdala-Valencia H, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:482. PubMed
12. Zhang K, et al. 2014. PLoS Pathog. 10:1004385. PubMed
FITC anti-mouse CD3e 货号：100306
FITC anti-mouse CD3e
货 号：100306
产品规格：500 ug
原 产 地：biolegend
参考价格：2470元 （参考价格，以实际价格为准）
优惠价格：
产品详细信息
FITC anti-mouse CD3e 100306 biolegend 细胞分群流式抗体
Clone: 145-2C11 (See other available formats)
Isotype: Armenian Hamster IgG
Isotype Control: FITC Armenian Hamster IgG Isotype Ctrl
Reactivity: Mouse
Immunogen: H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:
FC – Quality tested
Application
Notes:
The 145-2C11 antibody is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vivo T cell depletions. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6, in vitro and in vivo stimulation of T cells1,2,7,12,16, immunofluorescent staining5, and in vivo T cell depletions8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application
References:
1. Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activ)
2. Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. 3.12.1. (Activ)
3. Duke RC, et al. 1995. Current Protocols in Immunology. 3.17.1.
4. Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB)
5. Payer E, et al. 1991. J. Immunol. 146:2536. (IF)
6. Jacobs H, et al. 1994. Eur. J. Immunol. 24:934. (CMCD)
7. Vossen ACTM, et al. 1995. Eur. J. Immunol. 25:1492. (Activ)
8. Henrickson M, et al. 1995. Transplantation 60:828. (Deplete)
9. Kinnaert P, et al. 1996. Transpl. Int. 9:386. (Deplete)
10. Han WR, et al. 1999. Transpl. Immunol. 7:207. (Deplete)
11. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block)
12. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Activ)
产品详细信息
FITC anti-mouse CD3e 100306 biolegend 细胞分群流式抗体
Clone: 145-2C11 (See other available formats)
Isotype: Armenian Hamster IgG
Isotype Control: FITC Armenian Hamster IgG Isotype Ctrl
Reactivity: Mouse
Immunogen: H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:
FC – Quality tested
Application
Notes:
The 145-2C11 antibody is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vivo T cell depletions. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6, in vitro and in vivo stimulation of T cells1,2,7,12,16, immunofluorescent staining5, and in vivo T cell depletions8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application
References:
1. Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activ)
2. Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. 3.12.1. (Activ)
3. Duke RC, et al. 1995. Current Protocols in Immunology. 3.17.1.
4. Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB)
5. Payer E, et al. 1991. J. Immunol. 146:2536. (IF)
6. Jacobs H, et al. 1994. Eur. J. Immunol. 24:934. (CMCD)
7. Vossen ACTM, et al. 1995. Eur. J. Immunol. 25:1492. (Activ)
8. Henrickson M, et al. 1995. Transplantation 60:828. (Deplete)
9. Kinnaert P, et al. 1996. Transpl. Int. 9:386. (Deplete)
10. Han WR, et al. 1999. Transpl. Immunol. 7:207. (Deplete)
11. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block)
12. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Activ)
FITC anti-human CD45 Antibody 货号：304006
FITC anti-human CD45 Antibody
货 号：304006
产品规格：100 tests
原 产 地：biolegend
参考价格：1690 （参考价格，以实际价格为准）
优惠价格：
产品详细信息
FITC anti-human CD45 Antibody 304006 100 tests 细胞分群流式抗体
Clone: HI30 (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control: FITC Mouse IgG1, κ Isotype Ctrl
Workshop
Number: IV N816
Reactivity: Human, Cross-Reactivity: Chimpanzee
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Storage & Handling: The CD45 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
2. Kishihara K, et al. 1993. Cell 74:143.
3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
4. Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
5. Nagano M, et al. 2007. Blood 110:151.
6. Jiang Q, et al. 2008. Blood 112:2858. PubMed
7. Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
9. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
10. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
11. Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
产品详细信息
FITC anti-human CD45 Antibody 304006 100 tests 细胞分群流式抗体
Clone: HI30 (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control: FITC Mouse IgG1, κ Isotype Ctrl
Workshop
Number: IV N816
Reactivity: Human, Cross-Reactivity: Chimpanzee
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Storage & Handling: The CD45 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
2. Kishihara K, et al. 1993. Cell 74:143.
3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
4. Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
5. Nagano M, et al. 2007. Blood 110:151.
6. Jiang Q, et al. 2008. Blood 112:2858. PubMed
7. Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
9. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
10. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
11. Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
FITC anti-human CD3 货号：300406
FITC anti-human CD3
货 号：300406
产品规格：100 tests
原 产 地：USA
参考价格：1235 （参考价格，以实际价格为准）
优惠价格：
产品详细信息
300406 FITC anti-human CD3 100 tests
| Clone | UCHT1 |
| Isotype | Mouse IgG1, κ |
| Reactivity | Human, Cross-Reactivity: Chimpanzee |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA). |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC. |
| Recommended Usage |
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. ” |
| Applications |
FC – Quality tested |
| Application Notes |
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, and Western blotting9. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 300414). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300438) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg). |
| Application References |
1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP) ” |
| Description |
CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. |
| Storage | The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
产品详细信息
300406 FITC anti-human CD3 100 tests
| Clone | UCHT1 |
| Isotype | Mouse IgG1, κ |
| Reactivity | Human, Cross-Reactivity: Chimpanzee |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA). |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC. |
| Recommended Usage |
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. ” |
| Applications |
FC – Quality tested |
| Application Notes |
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, and Western blotting9. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 300414). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300438) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg). |
| Application References |
1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP) ” |
| Description |
CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. |
| Storage | The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
FITC Annexin V Apoptosis Detection Kit with 7-AAD 货号：640922
FITC Annexin V Apoptosis Detection Kit with 7-AAD
货 号：640922
产品规格：100T
原 产 地：USA
参考价格：2795 （参考价格，以实际价格为准）
优惠价格：2375
产品详细信息
FITC Annexin V Apoptosis Detection Kit with 7-AAD 640922 100T
Reactivity: All mammalian species
Storage & Handling: Store between 2°C and 8°C. Do not freeze.
Caution: 7-AAD is a potential carcinogen. It is recommended that the user wear protective clothing, gloves, and eye/face protection in order to avoid contact with skin and eyes.
Recommended Usage:
Staining Procedure:
1. Wash cells twice with cold BioLegend’s Cell Staining Buffer, and then resuspend cells in Annexin V Binding Buffer at a concentration of 0.25-1.0 x 107 cells/ml.
2. Transfer 100 µl of cell suspension in a 5 ml test tube.
3. Add 5 µl of FITC Annexin V.
4. Add 5 µl of 7-AAD Viability Staining Solution.
5. Gently vortex the cells and incubate for 15 min at room temperature (25°C) in the dark.
6. Add 400 µl of Annexin V Binding Buffer to each tube. Analyze by flow cytometry with proper machine settings.
产品详细信息
FITC Annexin V Apoptosis Detection Kit with 7-AAD 640922 100T
Reactivity: All mammalian species
Storage & Handling: Store between 2°C and 8°C. Do not freeze.
Caution: 7-AAD is a potential carcinogen. It is recommended that the user wear protective clothing, gloves, and eye/face protection in order to avoid contact with skin and eyes.
Recommended Usage:
Staining Procedure:
1. Wash cells twice with cold BioLegend’s Cell Staining Buffer, and then resuspend cells in Annexin V Binding Buffer at a concentration of 0.25-1.0 x 107 cells/ml.
2. Transfer 100 µl of cell suspension in a 5 ml test tube.
3. Add 5 µl of FITC Annexin V.
4. Add 5 µl of 7-AAD Viability Staining Solution.
5. Gently vortex the cells and incubate for 15 min at room temperature (25°C) in the dark.
6. Add 400 µl of Annexin V Binding Buffer to each tube. Analyze by flow cytometry with proper machine settings.