曲利苯蓝
基本信息
| 产品名称 | 曲利苯蓝 |
|---|---|
| 英文名称 | Trypan Blue |
| 别名 | 直接蓝 14；台盼蓝；C.I. 23850 |
| 英文别名 | Direct blue 14; Dianil blue; Diaminine Blue |
| 应用 | A viability dye for quantifying live versus dead cells |
| 规格或纯度 | Biological stain |
| 运输条件 | 常规运输 |
| 生化机理 | The trypan blue dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm. |
一般描述
Product Introduction
Trypan Blue is an azo based, hydrophilic, tetrasulfonated blue acid dye widely utilized for assessing cell viability. As an easy method to determining the viability of cells, Trypan Blue will stain dead cells with permeable membranes blue, while the dye is excluded by most living cells and their intact membranes thereby allowing visual determination of living versus dead cells. Although Trypan Blue is predominately used for assessing cell viability of cultured cells, other applications for this dye have been reported. In rat abdominal organ slices Trypan Blue has been utilized to determine cell viability as well as islet cell cluster cell viability from caprine pancreatic samples.
A viability dye for quantifying live versus dead cells
The trypan blue dye is an azo dye, derived from toluidine. It is soluble in water and has a molecular weight of 961 g/mol.
Application
Trypan blue has been used:
in cell viability assay
to determine the number of living and necrotic cells by staining with 0.2% trypan blue solution.
to stain plant tissue in order to to visualize cell death
Product Introduction
Trypan Blue is an azo based, hydrophilic, tetrasulfonated blue acid dye widely utilized for assessing cell viability. As an easy method to determining the viability of cells, Trypan Blue will stain dead cells with permeable membranes blue, while the dye is excluded by most living cells and their intact membranes thereby allowing visual determination of living versus dead cells. Although Trypan Blue is predominately used for assessing cell viability of cultured cells, other applications for this dye have been reported. In rat abdominal organ slices Trypan Blue has been utilized to determine cell viability as well as islet cell cluster cell viability from caprine pancreatic samples.
A viability dye for quantifying live versus dead cells
The trypan blue dye is an azo dye, derived from toluidine. It is soluble in water and has a molecular weight of 961 g/mol.
Application
Trypan blue has been used:
in cell viability assay
to determine the number of living and necrotic cells by staining with 0.2% trypan blue solution.
to stain plant tissue in order to to visualize cell death
安全信息
| 标识符号 |  GHS08 |
|---|---|
| 信号词 | Danger |
| 警示性声明 | P201,P308+P313 |
| 危害声明 | H350 |
| 个人防护设备 | Eyeshields,full-face particle respirator type N100 (US),Gloves,respirator cartridge type N100 (US),type P1 (EN143) respirator filter,type P3 (EN 143) respirator cartridges |
| WGK | 3 |
| Merck Index | 9792 |
相关属性
| CAS编号 | 72-57-1 |
|---|---|
| 熔点 | >300°C |
| 溶解性 | Soluble in water (10 mg/ml at 25 °C), ethanol (0.6 mg/ml), and EGME (20 mg/ml). |
| 储存温度 | 干燥 |
| RTECS | QJ6475000 |
| MDL号 | MFCD00003969 |
| 分子量 | 960.81 |
| 分子式 | C34H24N6O14S4Na4 |
| EC号 | 200-786-7 |
| 品牌 | Jinpan |
| Smiles | [Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1/N=Nc1c(O)c2c(N)cc(cc2cc1S(=O)(=O)[O-])S(=O)(=O)[O-])c1ccc(/N=Nc2c(O)c3c(N)cc(cc3cc2S(=O)(=O)[O-])S(=O)(=O)[O-])c(C)c1;Cc1cc(ccc1/N=N/c1c(O)c2c(N)cc(cc2cc1S(=O)(=O)O)S(=O)(=O)O)c1ccc(/N=N/c2c(O)c3c(N)cc(cc3cc2S(=O)(=O)O)S |
| PubChem CID | 6296 |