JC-10

JC-10是一种广泛用于检测线粒体膜电位△Ψm 的理想荧光探针。可以检测细胞、组织或纯化的线粒体膜电位。在线粒体膜电位较高时，JC-10聚集在线粒体的基质中，形成聚合物，可以产生红色荧光；在线粒体膜电位较低时，JC-10不能聚集在线粒体的基质中，此时JC-10为单体，可以产生绿色荧光。这样就可以非常方便地通过荧光颜色的转变来检测线粒体膜电位的变化。常用红绿荧光的相对比例来衡量线粒体去极化的比例。线粒体膜电位的下降是细胞凋亡早期的一个标志性事件。通过JC-10从红色荧光到绿色荧光的转变可以很容易地检测到细胞膜电位的下降，同时也可以用JC-10从红色荧光到绿色荧光的转变作为细胞凋亡早期的一个检测指标。JC-10单体的最大激发波长为515nm，最大发射波长为529nm；JC-10聚合物的最大激发波长为585nm，最大发射波长为590nm。实际观察时，使用常规的观察红色荧光和绿色荧光的设置即可。

JC-10 is an ideal fluorescent probe widely used to detect mitochondrial membrane potential △Ψm. Can detect cell, tissue or purified mitochondrial membrane potential. When the mitochondrial membrane potential is high, JC-10 aggregates in the matrix of the mitochondria to form a polymer, which can produce red fluorescence; when the mitochondrial membrane potential is low, JC-10 cannot accumulate in the matrix of the mitochondria, at this time JC- 10 is a monomer, which can produce green fluorescence. In this way, it is very convenient to detect the change of mitochondrial membrane potential through the change of fluorescence color. The relative ratio of red and green fluorescence is commonly used to measure the ratio of mitochondrial depolarization. The decrease of mitochondrial membrane potential is a landmark event in the early stage of apoptosis. The decrease of cell membrane potential can be easily detected by the transition of JC-10 from red fluorescence to green fluorescence, and the transition from red fluorescence to green fluorescence of JC-10 can also be used as an early detection indicator of cell apoptosis. The maximum excitation wavelength of JC-10 monomer is 515nm and the maximum emission wavelength is 529nm; the maximum excitation wavelength of JC-10 polymer is 585nm and the maximum emission wavelength is 590nm. For actual observation, use the conventional settings for observing red fluorescence and green fluorescence.