海洋硅藻培养基 不含硅酸 货号:G3454

海洋硅藻培养基 不含硅酸 货号:G3454

海洋硅藻培养基 不含硅酸

货  号:G3454

产品规格:50L

原  产  地:phytotechlab

参考价格:447 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

海洋硅藻培养基 不含硅酸  G3454  phytotechlab

f/2 Guillard’s Marine Enriched Seawater without Silicate
Product ID: G3454
 
Storage Temperature: 2 to 6°C
 
Description:
► f/2 Guillard’s Marine Enriched Seawater without Silicate
► A standard marine phytoplankton medium.
► Algal Culture Tested
► Soluble in seawater

Risk & Safety:
► R: 8-36/37/38
► S: 17-26-36

产品详细信息

海洋硅藻培养基 不含硅酸  G3454  phytotechlab

f/2 Guillard’s Marine Enriched Seawater without Silicate
Product ID: G3454
 
Storage Temperature: 2 to 6°C
 
Description:
► f/2 Guillard’s Marine Enriched Seawater without Silicate
► A standard marine phytoplankton medium.
► Algal Culture Tested
► Soluble in seawater

Risk & Safety:
► R: 8-36/37/38
► S: 17-26-36

GD基础培养基 货号:G371

GD基础培养基 货号:G371

GD基础培养基

货  号:G371

产品规格:50L

原  产  地:phytotechlab

参考价格:558 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

GD基础培养基  phytotechlab  G371

GRESSHOFF & DOY BASAL MEDIUM
Product ID: G371
 
Storage Temperature: 2 to 6°C
 
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS AND VITAMINS AS DESCRIBED BY GRESSHOFF & DOY (1974).
PLANT TISSUE CULTURE TESTED

TARIFF CODE: 3105.90.0000

产品详细信息

GD基础培养基  phytotechlab  G371

GRESSHOFF & DOY BASAL MEDIUM
Product ID: G371
 
Storage Temperature: 2 to 6°C
 
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS AND VITAMINS AS DESCRIBED BY GRESSHOFF & DOY (1974).
PLANT TISSUE CULTURE TESTED

TARIFF CODE: 3105.90.0000

B5培养基 货号:G398

B5培养基 货号:G398

B5培养基

货  号:G398

产品规格:50L

原  产  地:phytotechlab

参考价格:526 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

B5培养基  G398  phytotechlab

GAMBORG B-5 BASAL MEDIUM
Product ID: G398
 
Storage Temperature: 2 to 6°C
 
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS, AND VITAMINS AS DESCRIBED BY GAMBORG, ET AL. (1968).
PLANT TISSUE CULTURE TESTED

TARIFF CODE: 3105.90.0000

产品详细信息

B5培养基  G398  phytotechlab

GAMBORG B-5 BASAL MEDIUM
Product ID: G398
 
Storage Temperature: 2 to 6°C
 
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS, AND VITAMINS AS DESCRIBED BY GAMBORG, ET AL. (1968).
PLANT TISSUE CULTURE TESTED

TARIFF CODE: 3105.90.0000

B5培养基基础盐 货号:G768

B5培养基基础盐 货号:G768

B5培养基基础盐

货  号:G768

产品规格:50L

原  产  地:phytotechlab

参考价格:526 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

B5培养基基础盐  G768  phytotechlab

GAMBORG BASAL SALT MIXTURE (B-5 SALTS)
Product ID: G768
 
Storage Temperature: 2 to 6°C
 
Description:
CONTAINS THE MACRO- AND MICRONUTRIENTS AS DESCRIBED BY GAMBORG, ET AL. (1968).
THESE ARE THE SAME SALTS FOUND IN GAMBORG B-5 MEDIUM.
PLANT TISSUE CULTURE TESTED

TARIFF CODE: 3105.90.0000

产品详细信息

B5培养基基础盐  G768  phytotechlab

GAMBORG BASAL SALT MIXTURE (B-5 SALTS)
Product ID: G768
 
Storage Temperature: 2 to 6°C
 
Description:
CONTAINS THE MACRO- AND MICRONUTRIENTS AS DESCRIBED BY GAMBORG, ET AL. (1968).
THESE ARE THE SAME SALTS FOUND IN GAMBORG B-5 MEDIUM.
PLANT TISSUE CULTURE TESTED

TARIFF CODE: 3105.90.0000

BICINE 货号:0149-100G

BICINE 货号:0149-100G

BICINE

货  号:0149-100G

产品规格:100G

原  产  地:USA

参考价格:555 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

BICINE  0149-100G  BICINE

0149-100G BICINE BICINE ¥555 高纯级
0149-1KG BICINE BICINE ¥2,720 高纯级

产品详细信息

BICINE  0149-100G  BICINE

0149-100G BICINE BICINE ¥555 高纯级
0149-1KG BICINE BICINE ¥2,720 高纯级

抗坏血酸(维生素C) 货号:0143-100G

抗坏血酸(维生素C) 货号:0143-100G

抗坏血酸(维生素C)

货  号:0143-100G

产品规格:100G

原  产  地:USA

参考价格:298 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

抗坏血酸(维生素C)  0143-100G  L-ASCORBIC ACID, FREE ACID

0143-100G L-ASCORBIC ACID, FREE ACID 抗坏血酸(维生素C) ¥298 ACS级
0143-500G L-ASCORBIC ACID, FREE ACID 抗坏血酸(维生素C) ¥850 ACS级

产品详细信息

抗坏血酸(维生素C)  0143-100G  L-ASCORBIC ACID, FREE ACID

0143-100G L-ASCORBIC ACID, FREE ACID 抗坏血酸(维生素C) ¥298 ACS级
0143-500G L-ASCORBIC ACID, FREE ACID 抗坏血酸(维生素C) ¥850 ACS级

醋酸镁 货号:0131-500G

醋酸镁 货号:0131-500G

醋酸镁

货  号:0131-500G

产品规格:500G

原  产  地:USA

参考价格:395 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

醋酸镁  0131-500G  MAGNESIUM ACETATE TETRAHYDRATE

0131-1KG MAGNESIUM ACETATE TETRAHYDRATE 醋酸镁 ¥660 分析级
0131-2.5KG MAGNESIUM ACETATE TETRAHYDRATE 醋酸镁 ¥1,490 分析级
0131-500G MAGNESIUM ACETATE TETRAHYDRATE 醋酸镁 ¥395 分析级

产品详细信息

醋酸镁  0131-500G  MAGNESIUM ACETATE TETRAHYDRATE

0131-1KG MAGNESIUM ACETATE TETRAHYDRATE 醋酸镁 ¥660 分析级
0131-2.5KG MAGNESIUM ACETATE TETRAHYDRATE 醋酸镁 ¥1,490 分析级
0131-500G MAGNESIUM ACETATE TETRAHYDRATE 醋酸镁 ¥395 分析级

QIAGEN PCR Cloning Kit (40) 货号:231124

QIAGEN PCR Cloning Kit (40) 货号:231124

QIAGEN PCR Cloning Kit (40)

货  号:231124

产品规格:40

原  产  地:德国

参考价格:4410 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

QIAGEN PCR Cloning Kit (40)

231124

直接克隆Taq DNA聚合酶产生的PCR产物

  • 从PCR产物到涂板只需40分钟
  • 易用的连接酶预混液
  • 高特异性UA连接和高效克隆
  • 转化细胞可立刻涂板

QIAGEN PCR Cloning Kit提供即用型连接反应,包括3’端含有U突出末端的线性克隆载体,可直接进行高效、快速的连接和克隆。

原理

pDrive Cloning Vector(参见pDrive Cloning Vector)通过UA杂交可对PCR产物进行高效克隆。载体是3’端带有U突出末端的线性形式,能与Taq和 其他非高保真DNA聚合酶产生的带有A突出末端的PCR产物进行高特异性杂交。pDrive Cloning Vector具有很多有用的特征,便于对克隆的PCR产物进行分析。这些特征包括多种独特的限制性酶切识别位点、通用测序引物位点和用于体外转录的启动 子。此外,载体还可进行氨苄青霉素和卡那霉素筛选,以及对重组克隆的蓝/白斑筛选。

PCR产物可高效克隆到pDrive Cloning Vector,与基于TA的克隆载体相比,所需时间更少。而且,T是最可能与非互补碱基(如G、C和T)杂交的碱基,带有T突出末端的载体更可能发生自连 或连接克隆引物或退火引物,导致假阳性克隆数目增加。相比而言,pDrive Cloning Vector具有更高的克隆效率,表明U对非特异性碱基配对有更低的容忍度。

Ligation Master Mix采用预混形式,专用于为高效克隆提供最佳的杂交环境。

QIAGEN PCR Cloning Kit的组成
成分 包含 浓度
pDrive Cloning Vector QIAGEN PCR Cloning Kit (40) 货号:231124 50 ng/µl
Ligation Master Mix QIAGEN PCR Cloning Kit (40) 货号:231124 2x solution
Distilled water QIAGEN PCR Cloning Kit (40) 货号:231124
QIAGEN EZ Competent Cells
SOC medium

操作流程

 直接将PCR产物和pDrive Cloning Vector及Ligation Master Mix简单混合,再将连接反应液加入到感受态细胞中进行转化。

与拓扑异构酶法、TA法、常规粘性末端和平末端克隆方法相比,QIAGEN PCR Cloning Kit操作流程更加快速。连接需要30分钟,使用QIAGEN EZ Competent Cells进行转化和涂板只需10分钟,从PCR产物到涂板的整个操作流程只需40分钟。

应用
QIAGEN PCR Cloning Kit适用于克隆任何3’端带有单独A突出末端的PCR产物。使用Taq和 其他非高保真DNA聚合酶产生的PCR产物无需任何制备,可直接进行克隆。提供高保真DNA聚合酶的试剂盒,如HotStar HiFidelity Polymerase Kit和QIAGEN LongRange PCR Kit,可产生带有A突出末端的PCR产物,非常适合直接与QIAGEN PCR Cloning Kit配合使用。

产品详细信息

QIAGEN PCR Cloning Kit (40)

231124

直接克隆Taq DNA聚合酶产生的PCR产物

  • 从PCR产物到涂板只需40分钟
  • 易用的连接酶预混液
  • 高特异性UA连接和高效克隆
  • 转化细胞可立刻涂板

QIAGEN PCR Cloning Kit提供即用型连接反应,包括3’端含有U突出末端的线性克隆载体,可直接进行高效、快速的连接和克隆。

原理

pDrive Cloning Vector(参见pDrive Cloning Vector)通过UA杂交可对PCR产物进行高效克隆。载体是3’端带有U突出末端的线性形式,能与Taq和 其他非高保真DNA聚合酶产生的带有A突出末端的PCR产物进行高特异性杂交。pDrive Cloning Vector具有很多有用的特征,便于对克隆的PCR产物进行分析。这些特征包括多种独特的限制性酶切识别位点、通用测序引物位点和用于体外转录的启动 子。此外,载体还可进行氨苄青霉素和卡那霉素筛选,以及对重组克隆的蓝/白斑筛选。

PCR产物可高效克隆到pDrive Cloning Vector,与基于TA的克隆载体相比,所需时间更少。而且,T是最可能与非互补碱基(如G、C和T)杂交的碱基,带有T突出末端的载体更可能发生自连 或连接克隆引物或退火引物,导致假阳性克隆数目增加。相比而言,pDrive Cloning Vector具有更高的克隆效率,表明U对非特异性碱基配对有更低的容忍度。

Ligation Master Mix采用预混形式,专用于为高效克隆提供最佳的杂交环境。

QIAGEN PCR Cloning Kit的组成
成分 包含 浓度
pDrive Cloning Vector QIAGEN PCR Cloning Kit (40) 货号:231124 50 ng/µl
Ligation Master Mix QIAGEN PCR Cloning Kit (40) 货号:231124 2x solution
Distilled water QIAGEN PCR Cloning Kit (40) 货号:231124
QIAGEN EZ Competent Cells
SOC medium

操作流程

 直接将PCR产物和pDrive Cloning Vector及Ligation Master Mix简单混合,再将连接反应液加入到感受态细胞中进行转化。

与拓扑异构酶法、TA法、常规粘性末端和平末端克隆方法相比,QIAGEN PCR Cloning Kit操作流程更加快速。连接需要30分钟,使用QIAGEN EZ Competent Cells进行转化和涂板只需10分钟,从PCR产物到涂板的整个操作流程只需40分钟。

应用
QIAGEN PCR Cloning Kit适用于克隆任何3’端带有单独A突出末端的PCR产物。使用Taq和 其他非高保真DNA聚合酶产生的PCR产物无需任何制备,可直接进行克隆。提供高保真DNA聚合酶的试剂盒,如HotStar HiFidelity Polymerase Kit和QIAGEN LongRange PCR Kit,可产生带有A突出末端的PCR产物,非常适合直接与QIAGEN PCR Cloning Kit配合使用。

G418 G418硫酸盐 货号:11811-023

G418 G418硫酸盐 货号:11811-023

G418 G418硫酸盐

货  号:11811-023

产品规格:1g

原  产  地:USA

参考价格:867 (参考价格,以实际价格为准)

优惠价格:606

产品详细信息

G418 G418硫酸盐  11811-023 1g

Geneticin® Selective Antibiotic (G418 Sulfate), Powder

(Gibco®)
描述
晶体、未消毒(G418 硫酸盐)
稳定性: 非常稳定,可以粉末形式在室温下 (15°C 至 30°C) 保存最多 3 年;可溶于水。
常用规格
Validated Application:     

Eukaryotic Selection⁄Stable Cell Line Generation
Agent:     

Geneticin® (G-418)
Form:     

Powder
Reagent Type:     

Antibiotic (Selective)
Product Size:     

1 g
Shipping Condition:     

Room Temperature
Regulatory Statement:     

引文和参考文献 (144)

  • Efficient intracellular assembly of papillomaviral vectors.

作者
Buck CB, Pastrana DV, Lowy DR, Schiller JT,
杂志
J Virol (2004) 78:751-757
产品用途
Synthetic ORF encoding the BPV1 L1 protein was constructed with Invitrogen custom oligos purified with PAGE. Vector pUb6-V5-HisA was used to construct the target plasmids to express the ORF with Ecdysone inducible expression system. CV-1 and COS-7 cells were transfected using Lipofectamine Plus. 293 cells were transfected using Lipofectamine 2000. 
ID:
PN63752

  • The UL12.5 gene product of herpes simplex virus type 1 exhibits nuclease and strand exchange activities but does not localize to the nucleus.

作者
Reuven NB, Antoku S, Weller SK,
杂志
J Virol (2004) 78:4599-4608
产品用途
Sf9 cells were grown in Grace’s medium, D-MEM contained 5% FCS and pen-strep. The 6-5 cell line was maintained with G418. Restriction endonucleases, other DNA-modifying enzymes and DNA primers were purchased from Invitrogen. For preparation of a recombinant baculovirus expressing UL12.5, the Bac-to-Bac system was used. The cells were transfected with a total of 1 µg of plasmid DNA using Lipofectamine PLUS.  
ID:
PN65621

产品详细信息

G418 G418硫酸盐  11811-023 1g

Geneticin® Selective Antibiotic (G418 Sulfate), Powder

(Gibco®)
描述
晶体、未消毒(G418 硫酸盐)
稳定性: 非常稳定,可以粉末形式在室温下 (15°C 至 30°C) 保存最多 3 年;可溶于水。
常用规格
Validated Application:     

Eukaryotic Selection⁄Stable Cell Line Generation
Agent:     

Geneticin® (G-418)
Form:     

Powder
Reagent Type:     

Antibiotic (Selective)
Product Size:     

1 g
Shipping Condition:     

Room Temperature
Regulatory Statement:     

引文和参考文献 (144)

  • Efficient intracellular assembly of papillomaviral vectors.

作者
Buck CB, Pastrana DV, Lowy DR, Schiller JT,
杂志
J Virol (2004) 78:751-757
产品用途
Synthetic ORF encoding the BPV1 L1 protein was constructed with Invitrogen custom oligos purified with PAGE. Vector pUb6-V5-HisA was used to construct the target plasmids to express the ORF with Ecdysone inducible expression system. CV-1 and COS-7 cells were transfected using Lipofectamine Plus. 293 cells were transfected using Lipofectamine 2000. 
ID:
PN63752

  • The UL12.5 gene product of herpes simplex virus type 1 exhibits nuclease and strand exchange activities but does not localize to the nucleus.

作者
Reuven NB, Antoku S, Weller SK,
杂志
J Virol (2004) 78:4599-4608
产品用途
Sf9 cells were grown in Grace’s medium, D-MEM contained 5% FCS and pen-strep. The 6-5 cell line was maintained with G418. Restriction endonucleases, other DNA-modifying enzymes and DNA primers were purchased from Invitrogen. For preparation of a recombinant baculovirus expressing UL12.5, the Bac-to-Bac system was used. The cells were transfected with a total of 1 µg of plasmid DNA using Lipofectamine PLUS.  
ID:
PN65621