Anti-rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor 555 Conjugate) 货号:4413S

Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor 555 Conjugate) 货号:4413S

Anti-rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor 555 Conjugate)

货  号:4413S

产品规格:250 µl

原  产  地:USA

参考价格:1798 (参考价格,以实际价格为准)

优惠价格:

产品详细信息
Anti-rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor® 555 Conjugate) #4413
Confocal immunofluorescent analysis of HeLa cells labeled with MEK1/2 (47E6) Rabbit mAb #9126 detected with Anti-Rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor® 555 Conjugate) (red, left) compared to an isotype control (right). Actin filaments have been labeled with fluorescein phalloidin (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Product Usage Information

The optimal dilution of the anti-species antibody should be determined for each application. However, a final dilution of 1:1000 should yield acceptable results for most immunofluorescent applications.

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Product Description

Anti-Rabbit IgG (H+L) F(ab’)2 Fragment was conjugated to Alexa Fluor® 555 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab’)2 fragment results in less non-specific binding to cells through Fc receptors.

Specificity / Sensitivity

F(ab’)2 fragments are prepared from goat antibodies that have been adsorbed against pooled human serum, mouse serum, plasmacytoma/hybridoma proteins and purified human paraproteins.

产品详细信息 Anti-rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor® 555 Conjugate) #4413
Confocal immunofluorescent analysis of HeLa cells labeled with MEK1/2 (47E6) Rabbit mAb #9126 detected with Anti-Rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor® 555 Conjugate) (red, left) compared to an isotype control (right). Actin filaments have been labeled with fluorescein phalloidin (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Product Usage Information

The optimal dilution of the anti-species antibody should be determined for each application. However, a final dilution of 1:1000 should yield acceptable results for most immunofluorescent applications.

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Product Description

Anti-Rabbit IgG (H+L) F(ab’)2 Fragment was conjugated to Alexa Fluor® 555 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab’)2 fragment results in less non-specific binding to cells through Fc receptors.

Specificity / Sensitivity

F(ab’)2 fragments are prepared from goat antibodies that have been adsorbed against pooled human serum, mouse serum, plasmacytoma/hybridoma proteins and purified human paraproteins.

Anti-rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor® 488 Conjugate) 货号:4412S

Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) 货号:4412S

Anti-rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor® 488 Conjugate)

货  号:4412S

产品规格:250 µl

原  产  地:USA

参考价格:1798 (参考价格,以实际价格为准)

优惠价格:

产品详细信息
Anti-rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor® 488 Conjugate) #4412
The optimal dilution of the secondary antibody should be determined for each application. However, a final dilution of 1:1000 should yield acceptable results for most immunofluorescent applications.

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Product Description

Anti-Rabbit IgG (H+L) F(ab’)2 Fragment was conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab’)2 fragment results in less non-specific binding to cells through Fc receptors.

Specificity / Sensitivity

F(ab’)2 fragments are prepared from goat antibodies that have been adsorbed against pooled human serum, mouse serum, plasmacytoma/hybridoma proteins and purified human paraproteins.

产品详细信息 Anti-rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor® 488 Conjugate) #4412
The optimal dilution of the secondary antibody should be determined for each application. However, a final dilution of 1:1000 should yield acceptable results for most immunofluorescent applications.

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Product Description

Anti-Rabbit IgG (H+L) F(ab’)2 Fragment was conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab’)2 fragment results in less non-specific binding to cells through Fc receptors.

Specificity / Sensitivity

F(ab’)2 fragments are prepared from goat antibodies that have been adsorbed against pooled human serum, mouse serum, plasmacytoma/hybridoma proteins and purified human paraproteins.

10ul吸头 货号:T-300

10ul吸头 货号:T-300

10ul吸头

货  号:T-300

产品规格:1000支/包

原  产  地:axygen

参考价格:70 (参考价格,以实际价格为准)

优惠价格:

产品详细信息
货号 产品名称 销售包装 包装规格
T-300 10ul透明吸头 1000 Tips / 包,  20 包 / 箱 20
T-200-Y 200ul黄色吸头 1000 Tips / 包,  20 包 / 箱 20
T-1000-B 1000ul蓝色吸头 1000 Tips / 包, 5 包 / 箱 5
MCT-150-C 1.5ml无色离心管 500 Tubes / 包, 10 包 / 箱 10
PCR-02-C 0.2ml透明平盖PCR薄壁管 1000 Tubes / 包, 10 包 / 箱 10
PCR-0208-C 0.2ml 八连排透明PCR薄壁管 125 Strips / 包, 10 包 / 箱 10
PCR-2CP-RT-C 0.2ml透明PCR八排管平盖(荧光定量专用) 125 Strips / 包, 10 包 / 箱 10

产品详细信息

货号 产品名称 销售包装 包装规格
T-300 10ul透明吸头 1000 Tips / 包,  20 包 / 箱 20
T-200-Y 200ul黄色吸头 1000 Tips / 包,  20 包 / 箱 20
T-1000-B 1000ul蓝色吸头 1000 Tips / 包, 5 包 / 箱 5
MCT-150-C 1.5ml无色离心管 500 Tubes / 包, 10 包 / 箱 10
PCR-02-C 0.2ml透明平盖PCR薄壁管 1000 Tubes / 包, 10 包 / 箱 10
PCR-0208-C 0.2ml 八连排透明PCR薄壁管 125 Strips / 包, 10 包 / 箱 10
PCR-2CP-RT-C 0.2ml透明PCR八排管平盖(荧光定量专用) 125 Strips / 包, 10 包 / 箱 10

梯度降温盒,细胞冻存盒 货号:5100-0001

梯度降温盒,细胞冻存盒 货号:5100-0001

梯度降温盒,细胞冻存盒

货  号:5100-0001

产品规格:18孔

原  产  地:丹麦

参考价格:340 (参考价格,以实际价格为准)

优惠价格:

产品详细信息
细胞冻存盒,可实现每分钟1℃的降温,可大大的提高细胞的存活率,用之前在盒夹层内装入100%异丙醇,可装入18支冻存管。

产品编号 产品描述 产品包装 单位
5100-0001 直径*高度:120*86mm,适用于1.0-2.0ml冻存管 1 1

产品详细信息 细胞冻存盒,可实现每分钟1℃的降温,可大大的提高细胞的存活率,用之前在盒夹层内装入100%异丙醇,可装入18支冻存管。

产品编号 产品描述 产品包装 单位
5100-0001 直径*高度:120*86mm,适用于1.0-2.0ml冻存管 1 1

REPLI-g UltraFast Mini Kit (25)基因组扩增试剂盒 货号:150033

REPLI-g UltraFast Mini Kit (25)基因组扩增试剂盒 货号:150033

REPLI-g UltraFast Mini Kit (25)基因组扩增试剂盒

货  号:150033

产品规格:25T

原  产  地:德国

参考价格:3100 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

REPLI-g UltraFast Mini Kit (25)基因组扩增试剂盒

150033  25T

用于从珍贵或少量样本中超快速,高度均一地进行全基因组扩增
快速全基因组扩增,仅需1–1.5小时
获得无限量DNA适合多种下游应用
从各种起始样本中进行高重复性扩增
高度稳定的DNA适合长期储存

基因组DNA量不足以进行基因组分析,这一问题可通过样本内全基因组DNA扩增解决。REPLI-g UltraFast Kit包含REPLI-g UltraFast DNA Polymerase,该聚合酶可在60–90分钟内高度均一、正确的对少量样本进行全基因组扩增,通常20 μl反应体系产量可达7 μg。起始样本可以是基因组DNA、新鲜或干血、口腔拭子、新鲜或冷冻组织和培养细胞等。扩增得到的DNA可即用于多种基因分析。

DNA扩增产量高。
使用REPLI-g UltraFast Mini Kit对基因组DNA样本(每个10 ng)和无模板对照(NTC)样本(不含模板DNA)进行扩增,不同时间后测定DNA产量。扩增60分钟后的平均产量是7 µg,扩增90分钟后平均产量是10–13 µg。45分钟后即可获得多至5 µg DNA,可快速用于下游基因分析。在无模板对照反应中未检测到DNA。

原理

用于基因组分析的基因组DNA量通常十分有限。全基因组扩增技术通过对样本中的所有DNA进行全基因组扩增,为下游应用提供足量的DNA,克服这一问题。REPLI-g UltraFast技术可高度均一的进行全基因组扩增,序列偏差小。该方法基于MDA技术,采用独特的DNA聚合酶进行等温基因组扩增,可在较短时间内扩增得到长达100 kb的DNA产物,避免了基于PCR的扩增方法中常见的高序列偏差。此外,独特的碱性变性缓冲液确保了基因组DNA的温和变性,避免热变性导致的模板DNA片段化。因此可确保在下游应用中获得更加灵敏、更加可靠的结果。
操作流程
使用REPLI-g UltraFast Mini Kit进行全基因组扩增需要3个基本步骤。首先,对样本(10 ng纯化的基因组DNA、0.5 µl全血或300个组织培养细胞)进行温和的碱变性,避免模板DNA片段化。然后中和样本,加入REPLI-g UltraFast DNA Polymerase于30°C孵育。60分钟后可获得产物,扩增得到的DNA无需纯化,可即用于下游应用。

同类产品推荐:REPLI-g Midi Kit (25) 150034

REPLI-g UltraFast Mini Kit (100) 150035

产品详细信息

REPLI-g UltraFast Mini Kit (25)基因组扩增试剂盒

150033  25T

用于从珍贵或少量样本中超快速,高度均一地进行全基因组扩增
快速全基因组扩增,仅需1–1.5小时
获得无限量DNA适合多种下游应用
从各种起始样本中进行高重复性扩增
高度稳定的DNA适合长期储存

基因组DNA量不足以进行基因组分析,这一问题可通过样本内全基因组DNA扩增解决。REPLI-g UltraFast Kit包含REPLI-g UltraFast DNA Polymerase,该聚合酶可在60–90分钟内高度均一、正确的对少量样本进行全基因组扩增,通常20 μl反应体系产量可达7 μg。起始样本可以是基因组DNA、新鲜或干血、口腔拭子、新鲜或冷冻组织和培养细胞等。扩增得到的DNA可即用于多种基因分析。

DNA扩增产量高。
使用REPLI-g UltraFast Mini Kit对基因组DNA样本(每个10 ng)和无模板对照(NTC)样本(不含模板DNA)进行扩增,不同时间后测定DNA产量。扩增60分钟后的平均产量是7 µg,扩增90分钟后平均产量是10–13 µg。45分钟后即可获得多至5 µg DNA,可快速用于下游基因分析。在无模板对照反应中未检测到DNA。

原理

用于基因组分析的基因组DNA量通常十分有限。全基因组扩增技术通过对样本中的所有DNA进行全基因组扩增,为下游应用提供足量的DNA,克服这一问题。REPLI-g UltraFast技术可高度均一的进行全基因组扩增,序列偏差小。该方法基于MDA技术,采用独特的DNA聚合酶进行等温基因组扩增,可在较短时间内扩增得到长达100 kb的DNA产物,避免了基于PCR的扩增方法中常见的高序列偏差。此外,独特的碱性变性缓冲液确保了基因组DNA的温和变性,避免热变性导致的模板DNA片段化。因此可确保在下游应用中获得更加灵敏、更加可靠的结果。
操作流程
使用REPLI-g UltraFast Mini Kit进行全基因组扩增需要3个基本步骤。首先,对样本(10 ng纯化的基因组DNA、0.5 µl全血或300个组织培养细胞)进行温和的碱变性,避免模板DNA片段化。然后中和样本,加入REPLI-g UltraFast DNA Polymerase于30°C孵育。60分钟后可获得产物,扩增得到的DNA无需纯化,可即用于下游应用。

同类产品推荐:REPLI-g Midi Kit (25) 150034

REPLI-g UltraFast Mini Kit (100) 150035

1.8ml冻存管,2ml冻存管 货号:377267

1.8ml冻存管,2ml冻存管 货号:377267

1.8ml冻存管,2ml冻存管

货  号:377267

产品规格:

原  产  地:丹麦

参考价格:80 (参考价格,以实际价格为准)

优惠价格:

产品详细信息
品牌: NUNC
现货状态:
供应商: 金畔生物
数量: 大量
规格: 50支/包/36包/箱

NUNC冻存管,1.8ml冻存管,2ml冻存管内旋
NUNC冻存管377267 促销
冻存管,内旋盖,聚丙烯冻存管和内旋盖,已灭菌,具有书写区,建议工作容量,1.8ml

产品详细信息

品牌: NUNC
现货状态:
供应商: 金畔生物
数量: 大量
规格: 50支/包/36包/箱

NUNC冻存管,1.8ml冻存管,2ml冻存管内旋
NUNC冻存管377267 促销
冻存管,内旋盖,聚丙烯冻存管和内旋盖,已灭菌,具有书写区,建议工作容量,1.8ml

Anti-Human CD22 FITC 货号:11-0229-41

Anti-Human CD22 FITC 货号:11-0229-41

Anti-Human CD22 FITC

货  号:11-0229-41

产品规格:25tests

原  产  地:USA

参考价格:1188 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

Anti-Human CD22 FITC

11-0229-41
25tests

USA

The eBio4KB128 monoclonal antibody recognizes human CD22 (Siglec-2),which is a member

of the siglec subgroup of the Ig superfamily. CD22 is a type Itransmembrane glycoprotein
composed of two polypeptide chains, CD22α and CD22β, of 130 and 140 kDa respectively,
produced by alternative splicing of the CD22 gene. CD22 is expressed athigh levels on
mature B cells and B cell lymphomas. The extracellular portion of CD22contains seven
Ig-like domains, some of which are capable of binding ligands withsialic acid moieties
expressed on epithelial, endothelial, B and T cells. The intracellularportion of CD22
contains 6 tyrosine residues contained within immunotyrosine-basedinhibitory motifs (ITIM) and
immunotyrosine-based activation-like motifs, which are phosphorylatedupon B-cell receptor
engagement, which enables CD22 to participate in the positive andnegative regulation of
B-cell receptor signaling.

产品详细信息

Anti-Human CD22 FITC

11-0229-41
25tests

USA

The eBio4KB128 monoclonal antibody recognizes human CD22 (Siglec-2),which is a member

of the siglec subgroup of the Ig superfamily. CD22 is a type Itransmembrane glycoprotein
composed of two polypeptide chains, CD22α and CD22β, of 130 and 140 kDa respectively,
produced by alternative splicing of the CD22 gene. CD22 is expressed athigh levels on
mature B cells and B cell lymphomas. The extracellular portion of CD22contains seven
Ig-like domains, some of which are capable of binding ligands withsialic acid moieties
expressed on epithelial, endothelial, B and T cells. The intracellularportion of CD22
contains 6 tyrosine residues contained within immunotyrosine-basedinhibitory motifs (ITIM) and
immunotyrosine-based activation-like motifs, which are phosphorylatedupon B-cell receptor
engagement, which enables CD22 to participate in the positive andnegative regulation of
B-cell receptor signaling.

琼脂糖Agarose 货号:111860

琼脂糖Agarose 货号:111860

琼脂糖Agarose

货  号:111860

产品规格:100g

原  产  地:西班牙

参考价格:220 (参考价格,以实际价格为准)

优惠价格:

产品详细信息
数量: 大量
供应商: 上海金畔生物
品牌: 西班牙biowest
规格: 100g

西班牙琼脂糖,biowest琼脂糖Agarose
特价促销,欢迎寻购

  • 详细信息:
  • 参数: Regular
    EEO ——————————————-0.15
    Gel Strength(1%) (g/cm2)————≥750
    Gelling temperature (1%)(℃)——37±1.5 
    说明:用于日常核酸分析电泳、蛋白电泳等。

产品详细信息

数量: 大量
供应商: 上海金畔生物
品牌: 西班牙biowest
规格: 100g

西班牙琼脂糖,biowest琼脂糖Agarose
特价促销,欢迎寻购

  • 详细信息:
  • 参数: Regular
    EEO ——————————————-0.15
    Gel Strength(1%) (g/cm2)————≥750
    Gelling temperature (1%)(℃)——37±1.5 
    说明:用于日常核酸分析电泳、蛋白电泳等。

Anti-Mouse CD23 FITC 货号:11-0232-81

Anti-Mouse CD23 FITC 货号:11-0232-81

Anti-Mouse CD23 FITC

货  号:11-0232-81

产品规格:50ug

原  产  地:USA

参考价格:1068 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

Anti-Mouse CD23 FITC

11-0232-81

50ug

USA

The B3B4 monoclonal antibody reacts with mouse CD23, a 45 kDa type II transmembrane

glycoprotein. CD23 is expressed on resting conventional B cells, andits expression
is modulated upon B-cell activation. B-1 cell lineage (CD5+ B cells)does not express CD23.
Soluble forms of the antigen have been reported to be biologicallyactive. CD23 is a low
affinity receptor for IgE and is thought to play a role in theregulation of IgE response
and B-cell activation. CD21 is thought to bind to CD23.

产品详细信息

Anti-Mouse CD23 FITC

11-0232-81

50ug

USA

The B3B4 monoclonal antibody reacts with mouse CD23, a 45 kDa type II transmembrane

glycoprotein. CD23 is expressed on resting conventional B cells, andits expression
is modulated upon B-cell activation. B-1 cell lineage (CD5+ B cells)does not express CD23.
Soluble forms of the antigen have been reported to be biologicallyactive. CD23 is a low
affinity receptor for IgE and is thought to play a role in theregulation of IgE response
and B-cell activation. CD21 is thought to bind to CD23.

琼脂糖Agarose 货号:V900510

琼脂糖Agarose 货号:V900510

琼脂糖Agarose

货  号:V900510

产品规格:100g

原  产  地:SIGMA

参考价格:327 (参考价格,以实际价格为准)

优惠价格:

产品详细信息
牌: sigma
CAS号: 9012-36-6
供应商: 金畔生物
数量: 2
规格: 100g
琼脂糖
产品编号 V900510 | CAS号 9012-36-6 | Vetec
琼脂糖
Agarose
Vetec™ reagent grade

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content – used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength – the force that must be applied to a gel to cause it to fracture.
Gel point – the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition – that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) – a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

产品详细信息

牌: sigma
CAS号: 9012-36-6
供应商: 金畔生物
数量: 2
规格: 100g
琼脂糖
产品编号 V900510 | CAS号 9012-36-6 | Vetec
琼脂糖
Agarose
Vetec™ reagent grade

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content – used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength – the force that must be applied to a gel to cause it to fracture.
Gel point – the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition – that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) – a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.